FastQCFastQC Report
Tue 31 May 2016
SRR212753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424879
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG146253.4421564727840166No Hit
GTAATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG32250.7590396324600651No Hit
GTAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA29910.7039651288955209No Hit
GTAATGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG18430.4337705558523721No Hit
GTAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC15520.36528046808620807No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12230.2878466575189642No Hit
GTAATGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG11500.27066529529583716No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11030.2596033223576595No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC11020.2593679612313153No Hit
GTAATGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG10050.2365379319759273No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9680.22782957030119166No Hit
GTAATGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC9550.22476987565871695No Hit
GTAATGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC9180.21606151398398132No Hit
GTAATGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTC8530.2007630407716079No Hit
GTAATGGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT7290.17157826110492633No Hit
GTAATGGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT7070.16640031632535382No Hit
GTAATGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT6830.16075164929309285No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.1576919546506182No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.15345545437642247No Hit
GTAATGGGGAGGAAGGAATTCATGCCTGATAAAATAATCCTCTTCAAAAGTCCACA6190.14568853720706368No Hit
GTAATGGGGATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGACT5990.14098131468017952No Hit
GTAATGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5740.13509728652157438No Hit
GTAATGGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG5550.13062542512103445No Hit
GTAATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5410.12733036935221556No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4770.11226725726618637No Hit
GTAATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4710.11085509050812115No Hit
GTAATGGGGATAGCTTTCAGCTTTAAAACTATAAACATTAACAAAAATCCCACATT4530.10661859023392542No Hit
GTAATGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA4480.10544178460220438No Hit
GTAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4430.10426497897048335No Hit
GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC4350.10238208995972972No Hit
GTAATGGGGACACAATAAGAACTAGAAAAACGTCTCACACAGGAAGTGCCCCCAGT4330.1019113677070413No Hit
GTAATGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT4300.10120528432800868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG150.00826015850.25409350
TCGATAA150.00826403450.2481347
TATTCGG204.0799804E-450.2481348
TCTATTA150.00826403450.2481347
CGTAGCG252.0407251E-550.18262541
CGGTCTA204.1063302E-450.1826241
TCCGCAT204.108732E-450.17667439
CGACGTT150.00834964750.11729436
GCGACGT150.00836137250.09950335
CACGTAA150.00836137250.09950334
TACGTTA150.00836137250.09950334
AGACGAT204.147318E-450.08172233
TTCTACG150.008392750.05212831
CGTTACA150.008392750.05212831
CCTACGC150.00841625350.01665529
CCATATA204.1788805E-450.0048428
CACGTAC301.0456006E-650.0048428
AGCGCAA150.00842804849.99893626
AACGCTA150.00848720749.91053821
TAACGCT150.00850699649.8811420
Produced by FastQC (version 0.11.5)