FastQCFastQC Report
Tue 31 May 2016
SRR212752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390833
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG140043.583116062359115No Hit
GTAATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG28220.7220475241343489No Hit
GTAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA25820.6606402222944328No Hit
GTAATGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG17790.4551816248883794No Hit
GTAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC14550.3722817674044925No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11780.3014075065309224No Hit
GTAATGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG11660.2983371414389266No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11560.29577850386226345No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC10180.26046930530431156No Hit
GTAATGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC9320.2384650221450082No Hit
GTAATGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC9020.2307891094150187No Hit
GTAATGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG8950.22899806311135448No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8300.2123669188630438No Hit
GTAATGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC7890.2018765047987248No Hit
GTAATGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTC7420.18985090818840783No Hit
GTAATGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT6620.1693818075751024No Hit
GTAATGGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT6410.16400866866410974No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6020.15402998211512334No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.1504478895077949No Hit
GTAATGGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG5780.14788925193113173No Hit
GTAATGGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT5730.1466099331428001No Hit
GTAATGGGGATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGACT5470.13995747544347586No Hit
GTAATGGGGAGGAAGGAATTCATGCCTGATAAAATAATCCTCTTCAAAAGTCCACA5200.13304915398648529No Hit
GTAATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5080.12997878889448947No Hit
GTAATGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA4850.12409392246816416No Hit
GTAATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4720.12076769361850201No Hit
GTAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4600.1176973285265062No Hit
GTAATGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT4580.11718560101117356No Hit
GTAATGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT4460.11411523591917776No Hit
GTAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA4320.11053314331184931No Hit
GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC4070.10413654937019136No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4070.10413654937019136No Hit
GTAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3950.10106618427819555No Hit
GTAATGGGGATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAT3930.10055445676286291No Hit
GTAATGGGGATAGCTTTCAGCTTTAAAACTATAAACATTAACAAAAATCCCACATT3920.10029859300519658No Hit
GTAATGGGGTGTGTGTGTGTATGTGTGTGTGTATGTGTTTGTGTATGTGTGTATGT3910.10004272924753028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA150.008237774550.28783450
CACTATA204.066289E-450.28134546
AACGCTC204.066289E-450.28134549
GCTAACG150.00825460850.26187542
TCGACCG150.00825460850.26187543
CGTAGCG355.095535E-850.2229841
CGATGGA150.00831796150.16475736
TACCGTA355.142283E-850.16475738
CGTACAC204.1657005E-450.0358530
TACGACT150.00842863149.99730329
AACTATC150.00842863149.99730329
CATGCGA252.0887464E-549.9844728
ACGCGGT150.00847148749.93319725
TCGTAAA150.00852312749.85648320
ACCCGTG150.00852312749.85648320
AACCCGT150.00854039349.83096719
GCGTTTC204.2535894E-449.8245939
GATCTAG355.4254997E-849.8245939
GGATTCG402.759407E-949.8245938
TAAGCCG204.2535894E-449.82459318
Produced by FastQC (version 0.11.5)