FastQCFastQC Report
Tue 31 May 2016
SRR212751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308840
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA17260.5588654319388681No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT16280.5271337909597202No Hit
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11450.37074213184820615No Hit
AGATGGGGGATGAGAACAGAACCATTAACAGCCAGAGTGCCAATGGTGGGATCAGCAG11170.3616759487113068No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA7870.25482450459785005No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7460.24154902214739024No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6820.2208263178344774No Hit
AGATGGGGGATCCTGTCTACAAAACTGTCCTGGATATGTCTGGAAAGACCCAATGATG6780.21953114881492036No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6420.2078746276389069No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5280.17096231058153089No Hit
AGATGGGGGACAGGAAAATGGAGAATATCATTCTCTCTCCTCTGGGCTGATGATATTC5190.16804818028752752No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5060.16383888097396712No Hit
AGATGGGGGGGCCAAGGGAAGTAGTTCTGAAGGAAGATGGGGCATTAAGCTTTCTCAT4840.15671545136640333No Hit
AGATGGGGGGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGGAC4610.14926822950395027No Hit
AGATGGGGGATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGG4540.1470016837197254No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.12368864136769848No Hit
AGATGGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG3640.11786038077969176No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA3590.11624141950524543No Hit
AGATGGGGGGACCAGTCCCTTTAGGAAGGCTCAGTGGGGCTGTTTCCCTCTCTGCGTC3370.10911798989768165No Hit
AGATGGGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA3370.10911798989768165No Hit
AGATGGGGGATTTGTATTAGACACAAGTCTAAAGTGTTGTTTTTGTTTATTTTTGCAT3200.10361352156456419No Hit
AGATGGGGGGTACCTGTGGGTCGTGTGGGAGAGACATGAGTGGACCAGCGGGTCCAAC3200.10361352156456419No Hit
AGATGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3110.1006993912705608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGC307.9448364E-752.02949552
CGCTCAG150.007198999652.02949552
ACCGGTA150.00720361552.02106544
AATGCGA150.00720361552.02106548
CCGGTAT150.00720361552.02106545
CATTACG203.436834E-452.0210645
CCGATTA251.649982E-552.01263841
CGCGAAA150.00721285152.00421537
GGTATGT150.00721285152.00421536
GTACGTA150.00721285152.00421539
AGTACGT203.4423367E-452.00420838
CTTCGAA150.007217472451.9957923
CATATCG503.6379788E-1251.9957930
CACGATA251.6531492E-551.9957926
ATATCGG503.6379788E-1251.9957931
CCCGTCC503.6379788E-1251.9957920
CATACGT150.007217472451.9957921
CGTAACT150.007217472451.9957925
TAAATCC251.6531492E-551.9957927
TCGGTAG251.6531492E-551.9957926
Produced by FastQC (version 0.11.5)