FastQCFastQC Report
Tue 31 May 2016
SRR212750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307830
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA17030.5532274307247507No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT16180.5256147873826462No Hit
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11650.3784556411006075No Hit
AGATGGGGGATGAGAACAGAACCATTAACAGCCAGAGTGCCAATGGTGGGATCAGCAG10340.3358996848910113No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6980.2267485300328103No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6970.22642367540525615No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.22414969301237697No Hit
AGATGGGGGATCCTGTCTACAAAACTGTCCTGGATATGTCTGGAAAGACCCAATGATG6330.2056329792417893No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6060.1968619042978267No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5290.17184809797615566No Hit
AGATGGGGGACAGGAAAATGGAGAATATCATTCTCTCTCCTCTGGGCTGATGATATTC4970.16145274989442224No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4760.1546308027157847No Hit
AGATGGGGGGGCCAAGGGAAGTAGTTCTGAAGGAAGATGGGGCATTAAGCTTTCTCAT4550.14780885553714712No Hit
AGATGGGGGGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGGAC4230.1374135074554137No Hit
AGATGGGGGATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGG3930.12766786862878862No Hit
AGATGGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG3630.11792222980216353No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.11792222980216353No Hit
AGATGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3430.11142513725108014No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA3320.10785173634798428No Hit
AGATGGGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA3210.10427833544488842No Hit
AGATGGGGGATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAGAGTTT3130.10167949842445506No Hit
AGATGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.10102978916934671No Hit
AGATGGGGGGACCAGTCCCTTTAGGAAGGCTCAGTGGGGCTGTTTCCCTCTCTGCGTC3100.10070493454179255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACG150.007128315552.1594126
TCGGTAG150.007128315552.1594126
CGCTCAT203.3920349E-452.15940527
TTCGGTA150.007132916752.15089825
ATAGTCC150.007132916752.15089824
GCGTTAA150.00714212552.13389223
CGACGTG401.8480932E-952.1253922
CAACGCG251.6289481E-552.12538522
ATTCACG203.4167012E-452.08292850
ACGTAGG150.007169804552.08292852
CTATAGC150.00719293152.04053548
CATTACG203.4359843E-452.02359445
TCGATAG150.00722540251.98130430
AGCGATG150.00722540251.98130428
TTCGATA150.00722540251.98130429
GTATCAG251.6606502E-551.95595618
ATCGAAT251.6622487E-551.9475120
CACGAAT308.040679E-751.93907516
TGACACG353.9068254E-851.93907513
ACGTACA150.00724866251.93907512
Produced by FastQC (version 0.11.5)