FastQCFastQC Report
Tue 31 May 2016
SRR212749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306705
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA15090.4920037169266885No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT13860.4519000342348511No Hit
AGATGGGGGATGAGAACAGAACCATTAACAGCCAGAGTGCCAATGGTGGGATCAGCAG9750.31789504572797966No Hit
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9710.31659086092499306No Hit
AGATGGGGGATCCTGTCTACAAAACTGTCCTGGATATGTCTGGAAAGACCCAATGATG6580.2145384000912929No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6460.2106258456823332No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6380.20801747607636004No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6330.2063872450726268No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5460.17802122560766862No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4950.16139286936958966No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4900.15976263836585644No Hit
AGATGGGGGACAGGAAAATGGAGAATATCATTCTCTCTCCTCTGGGCTGATGATATTC4380.14280823592703085No Hit
AGATGGGGGGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGGAC4060.1323747575031382No Hit
AGATGGGGGGGCCAAGGGAAGTAGTTCTGAAGGAAGATGGGGCATTAAGCTTTCTCAT4040.1317226651016449No Hit
AGATGGGGGATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGG3740.12194127907924551No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.11313803165908609No Hit
AGATGGGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA3450.11248593925759282No Hit
AGATGGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG3090.10074827603071354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCC150.00704315352.31807710
GTACGTG150.00704315352.3180779
CGCATCG150.00719105652.0438925
CTGCGTC401.875378E-952.0353752
TAGTCGG150.007195714452.0353752
AGCCGAT150.007200375752.0268550
AGACGCG150.007200375752.0268528
GTCGGCG150.007200375752.0268544
AGGTATC150.007214372552.0013129
AGCTAAC203.4487972E-451.98429535
TAGCCGA150.0072283951.97579634
CTAGCCG150.00724711151.9418133
ATATACG353.920286E-851.9163522
ATATCGG150.007279959551.8824531
CCGACGT459.458745E-1151.8739821
TGATCGT150.00729407151.8570516
GCGAACT150.00729407151.8570517
TGGGTCG401.9335857E-951.8570517
AGCGAAC150.00729407151.8570516
TAAGGTA150.00729407151.8570517
Produced by FastQC (version 0.11.5)