FastQCFastQC Report
Tue 31 May 2016
SRR212748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295482
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC20850.7056267386845899No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT16400.5550253484137782No Hit
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10810.3658429278263989No Hit
AGATGGGGGATGAGAACAGAACCATTAACAGCCAGAGTGCCAATGGTGGGATCAGC8940.30255650090360836No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6500.21997955882253403No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.21084194637913645No Hit
AGATGGGGGATCCTGTCTACAAAACTGTCCTGGATATGTCTGGAAAGACCCAATGA6040.20441177465970856No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT6020.20373491447871614No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5990.20271962420722753No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4870.16481545407165243No Hit
AGATGGGGGACAGGAAAATGGAGAATATCATTCTCTCTCCTCTGGGCTGATGATAT4620.15635470180924727No Hit
AGATGGGGGGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGG4590.15533941153775865No Hit
AGATGGGGGGCTTTGCTGAACTTTGGAGAGTTGTTTTTTTATTAGTCAGTGTGGTG4050.1370641866509635No Hit
AGATGGGGGATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGA3930.13300302556500904No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.1306340149315356No Hit
AGATGGGGGGGCCAAGGGAAGTAGTTCTGAAGGAAGATGGGGCATTAAGCTTTCTC3540.11980425203565699No Hit
AGATGGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAA3320.11235879004474048No Hit
AGATGGGGGATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAGAGT3290.11134349977325184No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3240.10965134932077081No Hit
AGATGGGGGACTTGGGCAAGTCCCCTGCACATGACCTCATTTTAGGACCAAGAGCT3210.1086360590492822No Hit
AGATGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.10525175814432013No Hit
AGATGGGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT2980.10085216696786944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG150.00826293150.2459950
ATTACGG150.00829641750.19461446
GTAATCC204.0994343E-450.19461446
TCGCCAA150.00830200850.1860642
CTCCGAA150.00830200850.1860642
CATTACG150.00830200850.1860645
GTAGCGC150.00830200850.1860643
TGTAGCG150.00830200850.1860642
CATAAAC150.00830760150.17751341
CGTCGTC252.0424784E-550.1689740
CCGATGG150.00831319850.16896440
CGTTACG150.00831319850.16896440
ATTGCGG150.00831319850.16896440
CCGAGTA150.00831879750.16042339
TTATTAG650.050.16042339
ACTTCGA150.00832439950.15188238
CCCGTTG150.00833000350.14334537
AGTATCG204.1201632E-450.1433437
ATACCCC204.1201632E-450.1433437
GCGAAGT204.1236263E-450.13480436