SRR212748_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212748_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295482
Sequences flagged as poor quality	0
Sequence length	56
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	2085	0.7056267386845899	No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1640	0.5550253484137782	No Hit
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1081	0.3658429278263989	No Hit
AGATGGGGGATGAGAACAGAACCATTAACAGCCAGAGTGCCAATGGTGGGATCAGC	894	0.30255650090360836	No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	650	0.21997955882253403	No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	623	0.21084194637913645	No Hit
AGATGGGGGATCCTGTCTACAAAACTGTCCTGGATATGTCTGGAAAGACCCAATGA	604	0.20441177465970856	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	602	0.20373491447871614	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	599	0.20271962420722753	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	487	0.16481545407165243	No Hit
AGATGGGGGACAGGAAAATGGAGAATATCATTCTCTCTCCTCTGGGCTGATGATAT	462	0.15635470180924727	No Hit
AGATGGGGGGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGG	459	0.15533941153775865	No Hit
AGATGGGGGGCTTTGCTGAACTTTGGAGAGTTGTTTTTTTATTAGTCAGTGTGGTG	405	0.1370641866509635	No Hit
AGATGGGGGATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGA	393	0.13300302556500904	No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	386	0.1306340149315356	No Hit
AGATGGGGGGGCCAAGGGAAGTAGTTCTGAAGGAAGATGGGGCATTAAGCTTTCTC	354	0.11980425203565699	No Hit
AGATGGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAA	332	0.11235879004474048	No Hit
AGATGGGGGATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAGAGT	329	0.11134349977325184	No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	324	0.10965134932077081	No Hit
AGATGGGGGACTTGGGCAAGTCCCCTGCACATGACCTCATTTTAGGACCAAGAGCT	321	0.1086360590492822	No Hit
AGATGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	311	0.10525175814432013	No Hit
AGATGGGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT	298	0.10085216696786944	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAACG	15	0.008262931	50.24599	50
ATTACGG	15	0.008296417	50.194614	46
GTAATCC	20	4.0994343E-4	50.194614	46
TCGCCAA	15	0.008302008	50.18606	42
CTCCGAA	15	0.008302008	50.18606	42
CATTACG	15	0.008302008	50.18606	45
GTAGCGC	15	0.008302008	50.18606	43
TGTAGCG	15	0.008302008	50.18606	42
CATAAAC	15	0.008307601	50.177513	41
CGTCGTC	25	2.0424784E-5	50.16897	40
CCGATGG	15	0.008313198	50.168964	40
CGTTACG	15	0.008313198	50.168964	40
ATTGCGG	15	0.008313198	50.168964	40
CCGAGTA	15	0.008318797	50.160423	39
TTATTAG	65	0.0	50.160423	39
ACTTCGA	15	0.008324399	50.151882	38
CCCGTTG	15	0.008330003	50.143345	37
AGTATCG	20	4.1201632E-4	50.14334	37
ATACCCC	20	4.1201632E-4	50.14334	37
GCGAAGT	20	4.1236263E-4	50.134804	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content