FastQCFastQC Report
Tue 31 May 2016
SRR212747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263664
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC18590.7050640208750532No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14210.5389435038533892No Hit
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9450.358410704533042No Hit
AGATGGGGGATGAGAACAGAACCATTAACAGCCAGAGTGCCAATGGTGGGATCAGC7510.2848322106923964No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6110.2317343285393531No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5660.2146671521330178No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5580.21163298743855818No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5110.19380726985860794No Hit
AGATGGGGGATCCTGTCTACAAAACTGTCCTGGATATGTCTGGAAAGACCCAATGA4790.18167061108076946No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4390.1664997876084714No Hit
AGATGGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3920.14867407002852115No Hit
AGATGGGGGGCTTTGCTGAACTTTGGAGAGTTGTTTTTTTATTAGTCAGTGTGGTG3840.14563990533406151No Hit
AGATGGGGGGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGG3820.14488136416044664No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.13274470538260816No Hit
AGATGGGGGACAGGAAAATGGAGAATATCATTCTCTCTCCTCTGGGCTGATGATAT3300.12515929364645914No Hit
AGATGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC3170.12022877601796227No Hit
AGATGGGGGACTTGGGCAAGTCCCCTGCACATGACCTCATTTTAGGACCAAGAGCT3110.11795315249711755No Hit
AGATGGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT3080.1168153407366952No Hit
AGATGGGGGGGCCAAGGGAAGTAGTTCTGAAGGAAGATGGGGCATTAAGCTTTCTC3060.11605679956308028No Hit
AGATGGGGGATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGA3020.11453971721585048No Hit
AGATGGGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT2840.10771284665331633No Hit
AGATGGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAA2680.1016445172643971No Hit
AGATGGGGGATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAGAGT2650.10050670550397477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCGA150.00827922750.2194450
CAACATA355.1128154E-850.1906948
TACCATA204.1079373E-450.1715340
CGATTAG252.0412552E-550.1715342
GCCGCGA150.00831052750.1715345
CTCGAGC150.00831052750.1715338
CGTCATA150.00831052750.1715338
TGGTCGG150.00831052750.1715339
CCCGCAC150.00831052750.1715337
AAACTTA150.00836708950.08552635
CGGCTCG150.00841127950.01883331
CGCGTTT150.00841127950.01883330
ATATCGG252.0784086E-550.0188331
ACTAATG150.008430270549.99030329
TTCGCGT150.008430270549.99030328
GCCGTTA150.008430270549.99030329
TCGCGTT150.008430270549.99030329
TAGACTA150.008442949549.97130626
CGTGACA301.0502918E-649.96180725
CCGTCCG150.00844929549.96180724
Produced by FastQC (version 0.11.5)