SRR212746_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212746_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305475
Sequences flagged as poor quality	0
Sequence length	58
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	2874	0.9408298551436288	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2034	0.6658482690891235	No Hit
GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	1523	0.4985678042392995	No Hit
GCTGTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	1331	0.4357148702839839	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	1307	0.4278582535395695	No Hit
GCTGTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1141	0.373516654390703	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	860	0.2815287666748506	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	839	0.274654227023488	No Hit
GCTGTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	805	0.2635240199689009	No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	692	0.2265324494639496	No Hit
GCTGTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	686	0.22456829527784597	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	638	0.2088550617890171	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	634	0.20754562566494802	No Hit
GCTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	622	0.20361731729274082	No Hit
GCTGTGGGGAGTTGGACTTGTCCTTCGTTGTTTTCCAAAAATTCCGGGGATATTGTAT	577	0.18888616089696375	No Hit
GCTGTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	551	0.18037482609051478	No Hit
GCTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	479	0.15680497585727146	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	476	0.15582289876421965	No Hit
GCTGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	459	0.15025779523692612	No Hit
GCTGTGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG	445	0.14567476880268435	No Hit
GCTGTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	342	0.11195678860790573	No Hit
GCTGTGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA	336	0.1099926344218021	No Hit
GCTGTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG	329	0.10770112120468123	No Hit
GCTGTGGGGACTTTTGGCTCAGTTTGATACGGGAAGAAACCCCAAAGTTTTGGATTAG	326	0.10671904411162943	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	324	0.1060643260495949	No Hit
GCTGTGGGGACTTTTGTGAGTCAGATGCCAAAAAATGCATCGCTGTGGCCCCTCGCAT	320	0.10475488992552583	No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	319	0.10442753089450856	No Hit
GCTGTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	317	0.10377281283247401	No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	311	0.10180865864637041	No Hit
GCTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	306	0.10017186349128405	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAACG	25	1.6360793E-5	52.086792	51
CTAACGC	25	1.6360793E-5	52.086792	52
TCGAACT	30	7.8844096E-7	52.086792	52
CGTAGTC	20	3.420949E-4	52.069706	50
TACCTCG	25	1.6408527E-5	52.061172	43
TAGCCCG	20	3.4237205E-4	52.061165	45
CCGTAGT	20	3.4237205E-4	52.061165	49
CGTAAAT	20	3.4237205E-4	52.061165	49
CCGTTTG	40	1.8681021E-9	52.061165	45
TTACGCA	15	0.007190937	52.044098	42
TCAACGT	15	0.0072002662	52.02704	40
TAACCCA	15	0.007214277	52.001472	35
ACGCGAT	25	1.655242E-5	51.984447	24
GATACGG	25	1.655242E-5	51.984447	26
CGCGATC	25	1.655242E-5	51.984447	25
AACGCGA	50	5.456968E-12	51.984447	23
ACGTAAG	25	1.655242E-5	51.984447	31
TCGTTGT	80	0.0	51.984444	25
TGCGATC	55	0.0	51.984444	32
CAAATCG	20	3.4487434E-4	51.984444	25

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content