FastQCFastQC Report
Tue 31 May 2016
SRR212745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306385
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT29630.967083897710397No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19860.648204056987124No Hit
GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC14360.4686913523834392No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA13430.43833738596863425No Hit
GCTGTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT12320.40210845831225417No Hit
GCTGTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10180.3322616968846386No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA9230.3012549569985476No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA7980.26045661504316464No Hit
GCTGTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7450.24315811805408227No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7200.23499844966300568No Hit
GCTGTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6870.2242276873867846No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6410.20921389754720368No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.20692919039770225No Hit
GCTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6260.20431809651255775No Hit
GCTGTGGGGAGTTGGACTTGTCCTTCGTTGTTTTCCAAAAATTCCGGGGATATTGTAT5230.1707002627413222No Hit
GCTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4840.15797118005124272No Hit
GCTGTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4760.15536008616609823No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4360.14230461674037567No Hit
GCTGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4330.1413254565334465No Hit
GCTGTGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG3860.1259852799582225No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.11945754524536123No Hit
GCTGTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3370.10999232991171239No Hit
GCTGTGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3290.10738123602656788No Hit
GCTGTGGGGACTTTTGTGAGTCAGATGCCAAAAAATGCATCGCTGTGGCCCCTCGCAT3250.10607568908399562No Hit
GCTGTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3120.1018326615206358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG150.00716046852.10003326
CGCGAAC150.00716046852.10003325
ATACCCC150.00716046852.10003326
CACGAGA150.007183678452.0574250
CCTAACG251.6415615E-552.0574251
CCTATTA203.42495E-452.0574252
TATCGTG150.007183678452.0574252
CGTAAAT251.643152E-552.0489149
CGTGATA150.00718832752.04890448
TACGAAT150.00718832752.04890448
CGACGCT150.00720228752.02337639
AACTCGC150.00720228752.02337639
CCTCCGA150.007206944752.01487440
TAACGGA251.654321E-551.98938432
CGTAAGG203.447139E-451.98938432
CGTAACG251.654321E-551.98938430
ACGTAAG203.447139E-451.98938431
CGGTAGG650.051.98938431
TACGTAA203.447139E-451.98938430
AATCCCG150.007220931451.9893831
Produced by FastQC (version 0.11.5)