FastQCFastQC Report
Tue 31 May 2016
SRR212744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307871
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT26390.8571771943443844No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17380.5645221537592043No Hit
GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC13000.4222547755391056No Hit
GCTGTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT12980.4216051528075071No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA12480.4053645845175415No Hit
GCTGTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10230.33228202721269623No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA7810.2536776766892627No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7140.23191531518070882No Hit
GCTGTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7110.23094088108331087No Hit
GCTGTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6100.19813493313758035No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6080.19748531040598172No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5990.1945620081137879No Hit
GCTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5770.18741615806620304No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.18124474211601613No Hit
GCTGTGGGGAGTTGGACTTGTCCTTCGTTGTTTTCCAAAAATTCCGGGGATATTGTAT4800.15590945558366978No Hit
GCTGTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4540.14746436007288766No Hit
GCTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4250.13804483046470764No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3840.12472756446693582No Hit
GCTGTGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG3590.11660728032195301No Hit
GCTGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3560.1156328462245551No Hit
GCTGTGGGGACTTTTGGCTCAGTTTGATACGGGAAGAAACCCCAAAGTTTTGGATTAG3120.10134114612938537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC251.5820358E-552.3832669
AAGCGAT203.3210474E-452.38326610
GACGCAC203.3210474E-452.3832669
TGATACG503.6379788E-1252.0078225
TACGGGA503.6379788E-1252.0078228
TAACGGA203.4411266E-452.0078229
ACGGGAA503.6379788E-1252.0078229
GCCGACA150.0072201351.99088336
CGTTGCT203.4494378E-451.98241851
AATTCCG459.276846E-1151.9570440
AGTTCCG150.007238747651.9570440
ACACGTA203.460544E-451.94858623
GCCCAAT150.007248069651.94013246
ATAGCCG150.007248069651.94013248
CAACTCG150.007271413751.89792316
TACGGTG150.007276089451.88948415
TCACTAC150.007276089451.88948414
CACGTCA150.00728076751.88105422
CGTCTAT150.00728544751.87262321
ATCACGT150.007290129551.864218
Produced by FastQC (version 0.11.5)