FastQCFastQC Report
Tue 31 May 2016
SRR212743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315989
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT36351.1503564997515736No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17740.5614119478842619No Hit
GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG13780.43609112975451675No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT11240.35570858479250855No Hit
GCTGTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC10010.31678317916129994No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9810.3104538449123229No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA9810.3104538449123229No Hit
GCTGTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8250.2610850377703021No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8080.25570510365867166No Hit
GCTGTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC7190.2275395662507239No Hit
GCTGTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6880.21772909816480954No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.21677969802746297No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5850.18513302678257787No Hit
GCTGTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5570.17627195883401003No Hit
GCTGTGGGGAGTTGGACTTGTCCTTCGTTGTTTTCCAAAAATTCCGGGGATATTGT5100.16139802334891404No Hit
GCTGTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC4950.15665102266218128No Hit
GCTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4820.15253695540034623No Hit
GCTGTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4800.15190402197544853No Hit
GCTGTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA4540.1436758874517784No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4390.13892888676504564No Hit
GCTGTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA4090.12943488539158007No Hit
GCTGTGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG3880.12278908443015422No Hit
GCTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA3590.11361154976913754No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3480.11013041593220017No Hit
GCTGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA3380.10696574880771166No Hit
GCTGTGGGGATGTAAATCTGGCTGTCCTGGAGAATGTTATGTGAACCAGGCTGGCT3190.10095288127118349No Hit
GCTGTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT3160.10000348113383695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT150.00826694950.24063550
GTTCGAC150.00828259450.2166147
TGATTCG150.00828259450.2166147
CGGGTAT150.00829826250.19260846
CTTGCGG301.018072E-650.19260846
ACGCGCA150.00829826250.19260846
CGAAACG150.0083034950.18461244
GACGTCC204.1039084E-450.18461244
TACCTCG301.0191907E-650.18461243
CGCTTAG150.00835064750.11277439
ACGCTTA150.00836641150.08887538
TCGCTTG204.1460196E-450.0809135
CGGTTAG150.00838746250.0570430
CTACGTA252.073879E-550.04113429
TACGGGA301.0418025E-650.02524628
ATACGGG301.0440872E-650.00936527
TGATACG301.0463755E-649.99349625
ACGCGAT301.0486692E-649.97763424
TATTGCG150.00844026549.97763424
CTTCGTT550.049.9538623
Produced by FastQC (version 0.11.5)