FastQCFastQC Report
Tue 31 May 2016
SRR212742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290947
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT31141.0702980267883841No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15720.5403045915579126No Hit
GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG12640.4344433865961842No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT11080.38082537369349057No Hit
GCTGTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC10330.35504748287488785No Hit
GCTGTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC10310.35436007245305845No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA10060.3457674421801909No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC8990.3089909846123177No Hit
GCTGTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8480.29146201885566786No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC7150.24574922580401243No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6490.22306468188364204No Hit
GCTGTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5980.20553571612699223No Hit
GCTGTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA5640.1938497389558923No Hit
GCTGTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5330.1831948774175365No Hit
GCTGTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA5120.17597706798832777No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.17460224714466896No Hit
GCTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4930.16944666898094843No Hit
GCTGTGGGGAGTTGGACTTGTCCTTCGTTGTTTTCCAAAAATTCCGGGGATATTGT4420.1519177032242986No Hit
GCTGTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC4420.1519177032242986No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.13851319999862519No Hit
GCTGTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3910.13438873746764876No Hit
GCTGTGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG3680.12648351761661059No Hit
GCTGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA3620.12442128635112236No Hit
GCTGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT3480.11960941339831653No Hit
GCTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA3420.11754718213282832No Hit
GCTGTGGGGATGTAAATCTGGCTGTCCTGGAGAATGTTATGTGAACCAGGCTGGCT3340.11479754044551069No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.10173674243075201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAA150.00825509550.25784748
CGGCTAG150.00825509550.25784747
CTATTAG150.00826075650.24913446
AACCGTA204.0774202E-450.2491346
AAACCGT204.0809103E-450.2404245
CAAAACG150.008311829550.1708536
CCGCTTG150.008311829550.1708539
AAAACGC150.008311829550.1708537
TCGGCAT150.008311829550.1708536
AACGCCT150.008311829550.1708539
ATACTCG150.00832890650.1448135
ATAGAGC150.00835171650.1101333
CATTCCG402.6193447E-950.1101332
TGCAATC150.00835171650.1101332
ATAGGCC252.0629472E-550.08415231
CGTTAGG204.1441395E-450.08415231
AGTGCGA700.050.06685330
CCGATTG150.00841468250.0150128
AGACGAT204.1724902E-450.0150128
TTGACGC150.00842042550.00638627
Produced by FastQC (version 0.11.5)