FastQCFastQC Report
Tue 31 May 2016
SRR212741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28427
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGATTCGGGGACACCAGGTCACAGTGCTGGGGGAGATCAAAACCGGTAACT2430.8548211207654693No Hit
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.7739121257958983No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGA1140.40102719245787455No Hit
GGCTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.39750941006789325No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1120.39399162767791185No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT890.31308263270834064No Hit
GGCTGCGGGACATTACTCTGTGCCTAATGTTCCTCCATGTAGTTGATTTTTTTTCTTT880.30956485031835934No Hit
GGCTGCGGGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGA850.29901150314841524No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGGGT770.2708692440285644No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCTTGA710.24976254968867626No Hit
GGCTGCGGGAGCTCCAAAGACTTCCTTTAAAGAAGGCCTGGTCAGTAAACCAGCATCC700.2462447672986949No Hit
GGCTGCGGGCTGGCTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAA670.23569142012875083No Hit
GGCTGCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTT650.2286558553487881No Hit
GGCTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA640.22513807295880678No Hit
GGCTGCGGGGAACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAAAA630.2216202905688254No Hit
GGCTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.2145847257888627No Hit
GGCTGCGGGAGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATA600.21106694339888135No Hit
GGCTGCGGGACTAAAATCTGGGGATGGGAGGGCTCATTTGTTGATAAATAGCACTATT600.21106694339888135No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA590.20754916100889997No Hit
GGCTGCGGGGACGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAGAA570.20051359622893727No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.19347803144897457No Hit
GGCTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC530.18644246666901187No Hit
GGCTGCGGGGGGTTTCTAGTACATTCTTGGCTTTGCTATATCTATCTAGTGCCTGTTT520.1829246842790305No Hit
GGCTGCGGGCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATG510.17940690188904915No Hit
GGCTGCGGGAAGCATATCTCTATTTAATTTGATATTGGCTGTTAGTTTGCAATAAATT480.16885355471910507No Hit
GGCTGCGGGAAGAAAGAATTTGCCTTCTTATGCAATTTACAGCAGCATCAGCGTGATC480.16885355471910507No Hit
GGCTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT480.16885355471910507No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGTGT470.16533577232912372No Hit
GGCTGCGGGATGTTCATGTGTGTGTGTGTTTAGAAGCCACAAGATAATGATAATGTTG460.16181798993914237No Hit
GGCTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.15830020754916102No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC450.15830020754916102No Hit
GGCTGCGGGATTAAAATGTCTACGTAATCTTTCTTTCATATTGATTCTTCCTTTTCTT440.15478242515917967No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA440.15478242515917967No Hit
GGCTGCGGGGTTACCTCAGGCAGTCACACAGTTTGAAGAACTGGTTGGAATGGCAGAG410.1442290779892356No Hit
GGCTGCGGGATCCAGTATTGGGAAATCAGCAAATATTAGCTGGTAACCTACAGTGAGG400.14071129559925424No Hit
GGCTGCGGGATGGTTCTAAGTTGTTTTCATTTTAAAATGATACAATAATAGCATTTGT400.14071129559925424No Hit
GGCTGCGGGCTGCTCCAACCATTTTACGGTTTCCTTGTCAGTTGTTCTCTGCAGTTAC390.1371935132092729No Hit
GGCTGCGGGCTCTAACTACCCTGTTTTCCTTTCCAATTACTCTTCAAGAGAAACCATA380.13367573081929152No Hit
GGCTGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG380.13367573081929152No Hit
GGCTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG380.13367573081929152No Hit
GGCTGCGGGGCTAGAGGAAAATGTCCATGAGGTTCTGCAAGCGGTGGATGCTGGAGAC370.13015794842931017No Hit
GGCTGCGGGCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG360.12664016603932882No Hit
GGCTGCGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTT350.12312238364934745No Hit
GGCTGCGGGATTTGAGACAATGAGAATCCATGCATTGAGGGGAGAGAGGAAAGGAGGG350.12312238364934745No Hit
GGCTGCGGGATGTAATGTACACTGTAGCTATCCTCAGATACTCCAGAAGAGGGAGTCA350.12312238364934745No Hit
GGCTGCGGGATCTGGGGCTGTATGTAGGCCTTTGCGGGTGTTGTAGGTTTTTCTTTTT350.12312238364934745No Hit
GGCTGCGGGAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTCGCCTTCATGCGC340.1196046012593661No Hit
GGCTGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA340.1196046012593661No Hit
GGCTGCGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT330.11608681886938474No Hit
GGCTGCGGGATTAGGAAAGCTCTCAGCTTAGGGAAAGATGAAAGTTTTAGATCCTAGA320.11256903647940339No Hit
GGCTGCGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC320.11256903647940339No Hit
GGCTGCGGGAGCCCTGTGTGCCGACTGGATATTGAGCCCAGGTCCTCAAAGACACAGG320.11256903647940339No Hit
GGCTGCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.10905125408942204No Hit
GGCTGCGGGGAGGTGGGAGAGCTCTGTTAGCTCCCAAGAGACTTGGAGTAAGAAAGAT310.10905125408942204No Hit
GGCTGCGGGAACTGGGGTGTCTGAAATACTACTTAAAACTCAGATGAAGATGAGCCTC300.10553347169944068No Hit
GGCTGCGGGACTGCTGTGTCTGCCTGTTTTCCTTTCTTATAACATACAGAAAGCATGA300.10553347169944068No Hit
GGCTGCGGGGGAATATTAGGCTAATACCTAATATTACTTAGTAAAACTTGATTTTCAA300.10553347169944068No Hit
GGCTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG290.10201568930945933No Hit
GGCTGCGGGACTACTACTGAGCTAGAGCCTCAACACATCTAATCTTTTTACTTATTTT290.10201568930945933No Hit
GGCTGCGGGGTGAGATTCAGTGTAACTGTCTGAAATATTCAGAATGTTTGTGCATTTG290.10201568930945933No Hit
GGCTGCGGGATCTTTATCTCTTCCATTTCTCCAGCTTCTCTCCCTGAGCTACCAGAAA290.10201568930945933No Hit
GGCTGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC290.10201568930945933No Hit
GGCTGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT290.10201568930945933No Hit
GGCTGCGGGATTCTTTTTCTGTTTGGTGAAAAGATACCAGTAAAATGATAACATCTGG290.10201568930945933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCA150.007114640452.0617351
AGCCCAT150.007114640452.0617352
CAAGAGG150.007114640452.0617334
CAAGAAC150.007114640452.0617335
AAACCAG150.007114640452.0617347
CATTGCG150.007114640452.0617344
GTGCAGC150.007114640452.0617348
TGCGTAC150.007114640452.0617347
GTACCTG150.007114640452.0617350
CTCCCAC150.007114640452.0617347
CCAGCAT150.007114640452.0617350
AAACACC150.007114640452.0617339
AGGACGC150.007114640452.0617349
TTCAACA150.007114640452.0617349
TCGCGTG150.007114640452.0617344
AAGAGGG251.6029655E-552.0617347
CACGAGT150.007114640452.0617338
GGACACG150.007114640452.0617335
TAATGAC150.007114640452.0617347
TCAACAG150.007114640452.0617350