FastQCFastQC Report
Tue 31 May 2016
SRR212740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28748
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGATTCGGGGACACCAGGTCACAGTGCTGGGGGAGATCAAAACCGGTAACT2610.9078892444691804No Hit
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.8765827187978295No Hit
GGCTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.4382913593989147No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGA1230.4278558508417977No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1200.41742034228468067No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1110.38611381661332966No Hit
GGCTGCGGGACATTACTCTGTGCCTAATGTTCCTCCATGTAGTTGATTTTTTTTCTTT870.3026297481563935No Hit
GGCTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA790.27480172533741476No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGGGT760.26436621678029776No Hit
GGCTGCGGGCTGGCTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAA740.2574092110755531No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCTTGA740.2574092110755531No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA710.24697370251843606No Hit
GGCTGCGGGAGCTCCAAAGACTTCCTTTAAAGAAGGCCTGGTCAGTAAACCAGCATCC690.2400166968136914No Hit
GGCTGCGGGAGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATA670.23305969110894673No Hit
GGCTGCGGGGAACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAAAA660.22958118825657436No Hit
GGCTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.22610268540420203No Hit
GGCTGCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTT640.2226241825518297No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC640.2226241825518297No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGTGT610.2121886739947127No Hit
GGCTGCGGGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGA590.20523166828996803No Hit
GGCTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.19131765688047864No Hit
GGCTGCGGGGACGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAGAA540.1878391540281063No Hit
GGCTGCGGGATCCAGTATTGGGAAATCAGCAAATATTAGCTGGTAACCTACAGTGAGG500.17392514261861694No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.17392514261861694No Hit
GGCTGCGGGATTAAAATGTCTACGTAATCTTTCTTTCATATTGATTCTTCCTTTTCTT500.17392514261861694No Hit
GGCTGCGGGACTAAAATCTGGGGATGGGAGGGCTCATTTGTTGATAAATAGCACTATT500.17392514261861694No Hit
GGCTGCGGGAAGAAAGAATTTGCCTTCTTATGCAATTTACAGCAGCATCAGCGTGATC490.1704466397662446No Hit
GGCTGCGGGATGTTCATGTGTGTGTGTGTTTAGAAGCCACAAGATAATGATAATGTTG480.16696813691387227No Hit
GGCTGCGGGGGGTTTCTAGTACATTCTTGGCTTTGCTATATCTATCTAGTGCCTGTTT480.16696813691387227No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA480.16696813691387227No Hit
GGCTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG460.1600111312091276No Hit
GGCTGCGGGCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG460.1600111312091276No Hit
GGCTGCGGGCTGCTCCAACCATTTTACGGTTTCCTTGTCAGTTGTTCTCTGCAGTTAC450.15653262835675527No Hit
GGCTGCGGGGCTAGAGGAAAATGTCCATGAGGTTCTGCAAGCGGTGGATGCTGGAGAC450.15653262835675527No Hit
GGCTGCGGGGAGGTGGGAGAGCTCTGTTAGCTCCCAAGAGACTTGGAGTAAGAAAGAT450.15653262835675527No Hit
GGCTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT440.1530541255043829No Hit
GGCTGCGGGGTTACCTCAGGCAGTCACACAGTTTGAAGAACTGGTTGGAATGGCAGAG420.14609711979963824No Hit
GGCTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG370.12870460553777655No Hit
GGCTGCGGGGGAATATTAGGCTAATACCTAATATTACTTAGTAAAACTTGATTTTCAA370.12870460553777655No Hit
GGCTGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG370.12870460553777655No Hit
GGCTGCGGGAGGTAATTGTTTAGTTTATGATTTCAGATAATCATACCAGAGAACTTAA370.12870460553777655No Hit
GGCTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC370.12870460553777655No Hit
GGCTGCGGGAAGCATATCTCTATTTAATTTGATATTGGCTGTTAGTTTGCAATAAATT360.12522610268540418No Hit
GGCTGCGGGATGTAATGTACACTGTAGCTATCCTCAGATACTCCAGAAGAGGGAGTCA360.12522610268540418No Hit
GGCTGCGGGATGGTTCTAAGTTGTTTTCATTTTAAAATGATACAATAATAGCATTTGT350.12174759983303185No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG350.12174759983303185No Hit
GGCTGCGGGGCGTCTGGTTGGCCTAGGCTCAAGCAGCCGCGGCCTGGTTGGATCTGCT350.12174759983303185No Hit
GGCTGCGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTT340.11826909698065952No Hit
GGCTGCGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC340.11826909698065952No Hit
GGCTGCGGGATCTTTATCTCTTCCATTTCTCCAGCTTCTCTCCCTGAGCTACCAGAAA340.11826909698065952No Hit
GGCTGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT340.11826909698065952No Hit
GGCTGCGGGGGAAAAAGAATGCCAAAATCAGCAGCCTCCTGGAGGAGCAGTTCCAGCA330.11479059412828718No Hit
GGCTGCGGGCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATG320.11131209127591485No Hit
GGCTGCGGGAACTGGGGTGTCTGAAATACTACTTAAAACTCAGATGAAGATGAGCCTC320.11131209127591485No Hit
GGCTGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.11131209127591485No Hit
GGCTGCGGGGACTTGTAAGGACACAGCCAGAGCCTTGCATACCGACTGGAGTTTTGCA320.11131209127591485No Hit
GGCTGCGGGATCTGGGGCTGTATGTAGGCCTTTGCGGGTGTTGTAGGTTTTTCTTTTT320.11131209127591485No Hit
GGCTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1078335884235425No Hit
GGCTGCGGGAAGAATCCTATGCACCAACTGTTGACTTCCCCATCTGTTCCTCAACAGA300.10435508557117017No Hit
GGCTGCGGGGAGGAGGTATGGACAAAATAGATGTAATCTCTGTCATTCTGTGTCCAGG300.10435508557117017No Hit
GGCTGCGGGAGTTTCTAATATGCTATACAATTTATGCACGCAGAAAGAAATAGCAATG290.10087658271879783No Hit
GGCTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.10087658271879783No Hit
GGCTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCG290.10087658271879783No Hit
GGCTGCGGGGATGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAGAA290.10087658271879783No Hit
GGCTGCGGGATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGAC290.10087658271879783No Hit
GGCTGCGGGGCTTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG290.10087658271879783No Hit
GGCTGCGGGATTCTTTTTCTGTTTGGTGAAAAGATACCAGTAAAATGATAACATCTGG290.10087658271879783No Hit
GGCTGCGGGAGCCCTGTGTGCCGACTGGATATTGAGCCCAGGTCCTCAAAGACACAGG290.10087658271879783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG150.007106333552.07853323
CCTAATG150.007106333552.07853323
GCCTAAT150.007106333552.07853322
ATAGGAA150.007106333552.07853325
GGTCACA307.6426477E-752.07853324
CTAATGT150.007106333552.07853324
ATGTTCC150.007106333552.07853327
AGGTCAC307.6426477E-752.07853323
CAAGGTG150.007106333552.07853322
CTATGTG150.007106333552.07853321
GGGCGTT150.007106333552.07853327
CAGGTCA307.6426477E-752.07853322
ATCAACA150.007106333552.07853327
GCCTGTG150.007106333552.07853321
ATACATC150.007106333552.07853323
CCTAGGG150.007106333552.07853323
TACTATA150.007106333552.07853321
ACTATAG150.007106333552.07853322
GGTCCCG251.6003381E-552.0785326
GTCCCGT251.6003381E-552.0785327