SRR212739_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212739_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29184
Sequences flagged as poor quality	0
Sequence length	58
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTGCGGGATTCGGGGACACCAGGTCACAGTGCTGGGGGAGATCAAAACCGGTAACT	229	0.7846765350877192	No Hit
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	217	0.7435581140350876	No Hit
GGCTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.35293311403508776	No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGA	95	0.32552083333333337	No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	93	0.31866776315789475	No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	93	0.31866776315789475	No Hit
GGCTGCGGGACATTACTCTGTGCCTAATGTTCCTCCATGTAGTTGATTTTTTTTCTTT	77	0.26384320175438597	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	73	0.2501370614035088	No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCTTGA	70	0.2398574561403509	No Hit
GGCTGCGGGAGCTCCAAAGACTTCCTTTAAAGAAGGCCTGGTCAGTAAACCAGCATCC	69	0.23643092105263158	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGGGT	67	0.229577850877193	No Hit
GGCTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	67	0.229577850877193	No Hit
GGCTGCGGGGACGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAGAA	64	0.21929824561403508	No Hit
GGCTGCGGGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGA	63	0.21587171052631582	No Hit
GGCTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	61	0.2090186403508772	No Hit
GGCTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59	0.20216557017543862	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	55	0.1884594298245614	No Hit
GGCTGCGGGAGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATA	54	0.1850328947368421	No Hit
GGCTGCGGGGAACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAAAA	51	0.1747532894736842	No Hit
GGCTGCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTT	50	0.1713267543859649	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGTGT	50	0.1713267543859649	No Hit
GGCTGCGGGAAGAAAGAATTTGCCTTCTTATGCAATTTACAGCAGCATCAGCGTGATC	48	0.1644736842105263	No Hit
GGCTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48	0.1644736842105263	No Hit
GGCTGCGGGCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG	48	0.1644736842105263	No Hit
GGCTGCGGGCTGGCTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAA	46	0.1576206140350877	No Hit
GGCTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	45	0.15419407894736842	No Hit
GGCTGCGGGATCCAGTATTGGGAAATCAGCAAATATTAGCTGGTAACCTACAGTGAGG	44	0.1507675438596491	No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44	0.1507675438596491	No Hit
GGCTGCGGGCTGCTCCAACCATTTTACGGTTTCCTTGTCAGTTGTTCTCTGCAGTTAC	43	0.14734100877192982	No Hit
GGCTGCGGGATTAAAATGTCTACGTAATCTTTCTTTCATATTGATTCTTCCTTTTCTT	42	0.1439144736842105	No Hit
GGCTGCGGGAAGCATATCTCTATTTAATTTGATATTGGCTGTTAGTTTGCAATAAATT	42	0.1439144736842105	No Hit
GGCTGCGGGGGGTTTCTAGTACATTCTTGGCTTTGCTATATCTATCTAGTGCCTGTTT	42	0.1439144736842105	No Hit
GGCTGCGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTT	42	0.1439144736842105	No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	40	0.1370614035087719	No Hit
GGCTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	40	0.1370614035087719	No Hit
GGCTGCGGGAACTGGGGTGTCTGAAATACTACTTAAAACTCAGATGAAGATGAGCCTC	39	0.13363486842105263	No Hit
GGCTGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	39	0.13363486842105263	No Hit
GGCTGCGGGATGTTCATGTGTGTGTGTGTTTAGAAGCCACAAGATAATGATAATGTTG	37	0.12678179824561403	No Hit
GGCTGCGGGATGGTTCTAAGTTGTTTTCATTTTAAAATGATACAATAATAGCATTTGT	36	0.12335526315789473	No Hit
GGCTGCGGGACTAAAATCTGGGGATGGGAGGGCTCATTTGTTGATAAATAGCACTATT	36	0.12335526315789473	No Hit
GGCTGCGGGGAGGAGGTATGGACAAAATAGATGTAATCTCTGTCATTCTGTGTCCAGG	35	0.11992872807017545	No Hit
GGCTGCGGGGAGGTGGGAGAGCTCTGTTAGCTCCCAAGAGACTTGGAGTAAGAAAGAT	35	0.11992872807017545	No Hit
GGCTGCGGGATCACAGCATGGCTTGAGTGTGTCTATCCCTTTCTCAATCTTTGCCAAC	35	0.11992872807017545	No Hit
GGCTGCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC	34	0.11650219298245613	No Hit
GGCTGCGGGAGCAGGAGTCTACCAAAGCAGCTCTTTTGACCTACATCAAGCAGGAAGA	34	0.11650219298245613	No Hit
GGCTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCG	33	0.11307565789473685	No Hit
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA	33	0.11307565789473685	No Hit
GGCTGCGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC	33	0.11307565789473685	No Hit
GGCTGCGGGGTTACCTCAGGCAGTCACACAGTTTGAAGAACTGGTTGGAATGGCAGAG	32	0.10964912280701754	No Hit
GGCTGCGGGCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATG	32	0.10964912280701754	No Hit
GGCTGCGGGGTCTTAAGTCTTAAACCCCATCAGTTACATGAGTGACACACAATGAATG	32	0.10964912280701754	No Hit
GGCTGCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.10964912280701754	No Hit
GGCTGCGGGAGTTTCTAATATGCTATACAATTTATGCACGCAGAAAGAAATAGCAATG	31	0.10622258771929825	No Hit
GGCTGCGGGACCTTCTTTATATCTTACTTTAAATATTTTATGCATGTTTTCAAAAAAT	31	0.10622258771929825	No Hit
GGCTGCGGGCTCTAACTACCCTGTTTTCCTTTCCAATTACTCTTCAAGAGAAACCATA	30	0.10279605263157894	No Hit
GGCTGCGGGATCTTTATCTCTTCCATTTCTCCAGCTTCTCTCCCTGAGCTACCAGAAA	30	0.10279605263157894	No Hit
GGCTGCGGGATCTGGGGCTGTATGTAGGCCTTTGCGGGTGTTGTAGGTTTTTCTTTTT	30	0.10279605263157894	No Hit
GGCTGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30	0.10279605263157894	No Hit
GGCTGCGGGAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTCGCCTTCATGCGC	30	0.10279605263157894	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGCT	125	0.0	52.39688	5
GGATGGT	15	0.006937614	52.39688	8
ACTGCAT	15	0.006937614	52.39688	10
GGGTCAT	15	0.006937614	52.39688	8
GGGCACT	15	0.006937614	52.39688	7
GGATCTT	35	3.493369E-8	52.39688	8
GGATCAT	15	0.006937614	52.39688	8
GGATTCA	15	0.006937614	52.39688	8
GGCTGCT	30	7.329727E-7	52.39688	8
ACATTAC	15	0.006937614	52.39688	10
CGGGCTC	25	1.5439133E-5	52.39688	6
CGGGCAT	15	0.006937614	52.39688	6
CGGGCAC	15	0.006937614	52.39688	6
GCTGCTC	25	1.5439133E-5	52.39688	9
CGGGAGG	70	0.0	52.39688	6
CGGGACT	85	0.0	52.39688	6
CGGGACG	30	7.329727E-7	52.39688	6
GGGACTG	55	0.0	52.39688	7
GGGACCG	15	0.006937614	52.39688	7
GGGACAT	45	8.0035534E-11	52.39688	7

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content