SRR212738_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212738_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31593
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTGCGGGATTCGGGGACACCAGGTCACAGTGCTGGGGGAGATCAAAACCGGTAA	219	0.6931915297692527	No Hit
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	211	0.6678694647548508	No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT	161	0.5096065584148387	No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCTT	125	0.39565726585003	No Hit
GGCTGCGGGACTTTTCTGGAATGCAGCCTTTGCTCTCTCCGAGATGCCAGCCACGG	125	0.39565726585003	No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	123	0.3893267495964296	No Hit
GGCTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.33235210331402526	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	94	0.2975342639192226	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGG	93	0.29436900579242237	No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	91	0.2880384895388219	No Hit
GGCTGCGGGACATTACTCTGTGCCTAATGTTCCTCCATGTAGTTGATTTTTTTTCT	80	0.25322065014401923	No Hit
GGCTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	78	0.24689013389041875	No Hit
GGCTGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	76	0.2405596176368183	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGGGGG	68	0.21523755262241634	No Hit
GGCTGCGGGATTAAAATGTCTACGTAATCTTTCTTTCATATTGATTCTTCCTTTTC	65	0.20574177824201562	No Hit
GGCTGCGGGGACGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAG	64	0.2025765201152154	No Hit
GGCTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.1962460038616149	No Hit
GGCTGCGGGGAACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAA	61	0.19308074573481465	No Hit
GGCTGCGGGAGGATAGCAGTGGCCTGTGAGCTTCTGTAGCAATACCAGGAGACGCC	61	0.19308074573481465	No Hit
GGCTGCGGGAGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCA	59	0.1867502294812142	No Hit
GGCTGCGGGAAGAAAGAATTTGCCTTCTTATGCAATTTACAGCAGCATCAGCGTGA	57	0.18041971322761371	No Hit
GGCTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	56	0.17725445510081347	No Hit
GGCTGCGGGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTG	55	0.17408919697401323	No Hit
GGCTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	53	0.16775868072041275	No Hit
GGCTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.16775868072041275	No Hit
GGCTGCGGGAGCTCCAAAGACTTCCTTTAAAGAAGGCCTGGTCAGTAAACCAGCAT	51	0.16142816446681227	No Hit
GGCTGCGGGCTGGCTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTG	51	0.16142816446681227	No Hit
GGCTGCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC	49	0.1550976482132118	No Hit
GGCTGCGGGATGTTCATGTGTGTGTGTGTTTAGAAGCCACAAGATAATGATAATGT	49	0.1550976482132118	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	49	0.1550976482132118	No Hit
GGCTGCGGGCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA	49	0.1550976482132118	No Hit
GGCTGCGGGCTGCTCCAACCATTTTACGGTTTCCTTGTCAGTTGTTCTCTGCAGTT	48	0.15193239008641155	No Hit
GGCTGCGGGATTTACAGTTGGTGGTCTAACCGGAATTGTTTTATCCAACTCATCCC	47	0.1487671319596113	No Hit
GGCTGCGGGGGGTTTCTAGTACATTCTTGGCTTTGCTATATCTATCTAGTGCCTGT	47	0.1487671319596113	No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	46	0.14560187383281106	No Hit
GGCTGCGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATC	45	0.14243661570601082	No Hit
GGCTGCGGGGTTACCTCAGGCAGTCACACAGTTTGAAGAACTGGTTGGAATGGCAG	43	0.13610609945241034	No Hit
GGCTGCGGGACTGCTGTGTCTGCCTGTTTTCCTTTCTTATAACATACAGAAAGCAT	42	0.1329408413256101	No Hit
GGCTGCGGGGATGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAG	41	0.12977558319880986	No Hit
GGCTGCGGGATGTAATGTACACTGTAGCTATCCTCAGATACTCCAGAAGAGGGAGT	40	0.12661032507200962	No Hit
GGCTGCGGGCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCA	40	0.12661032507200962	No Hit
GGCTGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.12661032507200962	No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.12661032507200962	No Hit
GGCTGCGGGAACTGGGGTGTCTGAAATACTACTTAAAACTCAGATGAAGATGAGCC	38	0.12027980881840915	No Hit
GGCTGCGGGATCCAGTATTGGGAAATCAGCAAATATTAGCTGGTAACCTACAGTGA	37	0.11711455069160889	No Hit
GGCTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	36	0.11394929256480865	No Hit
GGCTGCGGGGTTCAAACCCACAAAACTCATGAAAATAGCTGGGCAAAATGAGTGTA	35	0.11078403443800842	No Hit
GGCTGCGGGATAGTTAATTTCATGGTTCTTAGAATTGTTAAATATATTCTTTTCAG	34	0.10761877631120817	No Hit
GGCTGCGGGATCTTTATCTCTTCCATTTCTCCAGCTTCTCTCCCTGAGCTACCAGA	34	0.10761877631120817	No Hit
GGCTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	34	0.10761877631120817	No Hit
GGCTGCGGGAAGCATATCTCTATTTAATTTGATATTGGCTGGTAGTTTGCAATAAA	33	0.10445351818440794	No Hit
GGCTGCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTAAATCTTCT	33	0.10445351818440794	No Hit
GGCTGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA	32	0.1012882600576077	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCCA	25	1.9498952E-5	50.386223	50
ACCGGTA	20	3.9675983E-4	50.38622	49
AACCGGT	20	3.9675983E-4	50.38622	48
TAGGAAG	20	3.9675983E-4	50.38622	50
TTCAACA	20	3.9675983E-4	50.38622	49
ATGACCC	20	3.9675983E-4	50.38622	49
TCAACAG	20	3.9675983E-4	50.38622	50
AATGACC	35	4.7721187E-8	50.38622	48
CCGGTAA	20	3.9675983E-4	50.38622	50
TCGCGTC	15	0.008104547	50.386215	48
GCAATAA	15	0.008104547	50.386215	49
GTTTAGG	20	3.999172E-4	50.3056	47
TCTTGCT	20	4.0309448E-4	50.225243	43
GTTTGCA	15	0.0082079545	50.22524	45
CTTTAAT	35	4.8947186E-8	50.22524	44
AGTGGCT	30	9.842806E-7	50.22524	39
ATGGTCG	15	0.0082079545	50.22524	44
GCTAACG	15	0.0082079545	50.22524	37
GGCTTTA	35	4.8947186E-8	50.22524	42
TTGTGTG	35	4.8947186E-8	50.22524	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content