SRR212737_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212737_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29413
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	207	0.703770441641451	No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT	184	0.625573725903512	No Hit
GGCTGCGGGATTCGGGGACACCAGGTCACAGTGCTGGGGGAGATCAAAACCGGTAA	160	0.5439771529595757	No Hit
GGCTGCGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCTT	124	0.42158229354367116	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGG	121	0.4113827219256791	No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	109	0.37058443545371095	No Hit
GGCTGCGGGACTTTTCTGGAATGCAGCCTTTGCTCTCTCCGAGATGCCAGCCACGG	106	0.3603848638357189	No Hit
GGCTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98	0.3331860061877401	No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	93	0.3161867201577534	No Hit
GGCTGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	91	0.30938700574575867	No Hit
GGCTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	86	0.29238771971577193	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	79	0.2685887192737905	No Hit
GGCTGCGGGAGGATAGCAGTGGCCTGTGAGCTTCTGTAGCAATACCAGGAGACGCC	78	0.2651888620677932	No Hit
GGCTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	72	0.24478971883180908	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGGGGG	71	0.24138986162581172	No Hit
GGCTGCGGGATGTTCATGTGTGTGTGTGTTTAGAAGCCACAAGATAATGATAATGT	68	0.23119029000781965	No Hit
GGCTGCGGGGAACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAA	62	0.21079114677183558	No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.19719171794784618	No Hit
GGCTGCGGGGACGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAG	57	0.19379186074184884	No Hit
GGCTGCGGGGGTGGTCCACGCCGAGCGACTGAGAAGCCCAGTCTGCGCCCTCAGGA	54	0.1835922891238568	No Hit
GGCTGCGGGAAGAAAGAATTTGCCTTCTTATGCAATTTACAGCAGCATCAGCGTGA	54	0.1835922891238568	No Hit
GGCTGCGGGAGCTCCAAAGACTTCCTTTAAAGAAGGCCTGGTCAGTAAACCAGCAT	53	0.18019243191785944	No Hit
GGCTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	53	0.18019243191785944	No Hit
GGCTGCGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATC	51	0.17339271750586477	No Hit
GGCTGCGGGACTAAAATCTGGGGATGGGAGGGCTCATTTGTTGATAAATAGCACTA	50	0.1699928602998674	No Hit
GGCTGCGGGCTGGCTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTG	50	0.1699928602998674	No Hit
GGCTGCGGGAGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCA	49	0.16659300309387004	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGTGTGGT	48	0.1631931458878727	No Hit
GGCTGCGGGACATTACTCTGTGCCTAATGTTCCTCCATGTAGTTGATTTTTTTTCT	47	0.15979328868187537	No Hit
GGCTGCGGGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTG	47	0.15979328868187537	No Hit
GGCTGCGGGATTAAAATGTCTACGTAATCTTTCTTTCATATTGATTCTTCCTTTTC	46	0.156393431475878	No Hit
GGCTGCGGGATTTACAGTTGGTGGTCTAACCGGAATTGTTTTATCCAACTCATCCC	46	0.156393431475878	No Hit
GGCTGCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC	45	0.15299357426988067	No Hit
GGCTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.15299357426988067	No Hit
GGCTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.15299357426988067	No Hit
GGCTGCGGGCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCA	44	0.14959371706388333	No Hit
GGCTGCGGGATGTAATGTACACTGTAGCTATCCTCAGATACTCCAGAAGAGGGAGT	42	0.14279400265188863	No Hit
GGCTGCGGGCTGCTCCAACCATTTTACGGTTTCCTTGTCAGTTGTTCTCTGCAGTT	42	0.14279400265188863	No Hit
GGCTGCGGGAAGTGGTCACTTTGGCAACCCCTCGGGGATGACAGTGGTGACGCTAT	42	0.14279400265188863	No Hit
GGCTGCGGGGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGACTTAATGTATCTTA	42	0.14279400265188863	No Hit
GGCTGCGGGGATGGAGGGGAGATTGAGCGATGCTCTTTCCTTCTCTGTTTAGGAAG	41	0.13939414544589127	No Hit
GGCTGCGGGGGGTTTCTAGTACATTCTTGGCTTTGCTATATCTATCTAGTGCCTGT	41	0.13939414544589127	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	40	0.13599428823989393	No Hit
GGCTGCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTAAATCTTCT	39	0.1325944310338966	No Hit
GGCTGCGGGCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA	38	0.12919457382789923	No Hit
GGCTGCGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC	36	0.12239485941590454	No Hit
GGCTGCGGGAACTGGGGTGTCTGAAATACTACTTAAAACTCAGATGAAGATGAGCC	36	0.12239485941590454	No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	34	0.11559514500390983	No Hit
GGCTGCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	33	0.11219528779791248	No Hit
GGCTGCGGGGTTACCTCAGGCAGTCACACAGTTTGAAGAACTGGTTGGAATGGCAG	33	0.11219528779791248	No Hit
GGCTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	32	0.10879543059191515	No Hit
GGCTGCGGGATGGTTCTAAGTTGTTTTCATTTTAAAATGATACAATAATAGCATTT	32	0.10879543059191515	No Hit
GGCTGCGGGGTGTCTGTGTGTGTGTGTGTCTGTGTGTGTGTCTGTTTGTGGGGGGG	32	0.10879543059191515	No Hit
GGCTGCGGGGTTCAAACCCACAAAACTCATGAAAATAGCTGGGCAAAATGAGTGTA	31	0.10539557338591779	No Hit
GGCTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	31	0.10539557338591779	No Hit
GGCTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	30	0.10199571617992044	No Hit
GGCTGCGGGACTGCATGGGCCTCACAGAGATGGAATGTGCTTCCAGACAGAGGTCT	30	0.10199571617992044	No Hit
GGCTGCGGGGAGGTGGGAGAGCTCTGTTAGCTCCCAAGAGACTTGGAGTAAGAAAG	30	0.10199571617992044	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGCGC	15	0.008079713	50.416523	50
CCGGTAA	25	1.9396133E-5	50.416523	50
TGAGAGC	15	0.008079713	50.416523	50
TAGGAAG	20	3.9512853E-4	50.41652	50
ACCGGTA	25	1.9595314E-5	50.3299	49
GTTTGTG	50	5.456968E-12	50.3299	44
AACCGGT	25	1.9595314E-5	50.3299	48
ATGACCC	25	1.9595314E-5	50.3299	49
GTCTGTT	50	5.456968E-12	50.3299	40
CTGTTTG	50	5.456968E-12	50.3299	42
CTTTTGG	15	0.008134963	50.329895	46
CCGGAAG	15	0.008134963	50.329895	49
TAAACCA	15	0.008134963	50.329895	46
GCAAGCG	15	0.008134963	50.329895	49
GTCTGAT	15	0.008134963	50.329895	41
TAATGAC	40	2.386514E-9	50.329895	47
CTGATCT	15	0.008134963	50.329895	43
GATGAGC	20	3.9850725E-4	50.329895	49
ACTTTTG	15	0.008134963	50.329895	45
CAATACC	20	3.9850725E-4	50.329895	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content