FastQCFastQC Report
Tue 31 May 2016
SRR212736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159559
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7760.48634047593680074No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7020.4399626470459203No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5630.3528475360211583No Hit
AACGCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4750.2976955232860572No Hit
AACGCCGGGATTCTTTACCCTGTAGACTTTTTTGAGGGGCGTATGCTCTGTAGCACTA4320.27074624433595096No Hit
AACGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4230.26510569757895197No Hit
AACGCCGGGGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGG4210.2638522427440633No Hit
AACGCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3930.24630387505562207No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3900.24442369280328907No Hit
AACGCCGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGAAA3590.2249951428625148No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC3510.21998132352296015No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3450.21622095901829416No Hit
AACGCCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACA2970.18613804298096628No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2930.18363113331118897No Hit
AACGCCGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGCA2900.18175095105885597No Hit
AACGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2860.17924404138907865No Hit
AACGCCGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT2720.17046985754485802No Hit
AACGCCGGGCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAG2610.1635758559529704No Hit
AACGCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT2550.15981549144830437No Hit
AACGCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2410.15104130760408377No Hit
AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2390.1497878527691951No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.1497878527691951No Hit
AACGCCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2350.14728094309941778No Hit
AACGCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.1460274882645291No Hit
AACGCCGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTTAA2290.14352057859475179No Hit
AACGCCGGGATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGGCT2280.14289385117730743No Hit
AACGCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2100.13161275766330952No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.12973257541097652No Hit
AACGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2020.12659893832375485No Hit
AACGCCGGGGCTGAACCTTCATTAGACTCTTTCCTTGTACCCCTCCCCTACCCATTTC1990.12471875607142187No Hit
AACGCCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA1990.12471875607142187No Hit
AACGCCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1970.1234653012365332No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.12283857381908886No Hit
AACGCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC1920.12033166414931154No Hit
AACGCCGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACT1850.11594457222720123No Hit
AACGCCGGGAGTTATCTAGGTTAACAACCCTCTCGAGTATTTGCTGTCTGTCAAGTTC1850.11594457222720123No Hit
AACGCCGGGATGCATATTGTATGTATCTTGTTACCTACTCATCCAGCCGTGGGTACCT1810.1134376625574239No Hit
AACGCCGGGGTACAAATGTGTTTCTCAGTGTGGGTTTTTGTTTTTTGTTGGGTTTTTT1800.11281093513997957No Hit
AACGCCGGGAGAAGAGGAAGTTCAGAACTCAGTTTCTGGGGAAAGTGAAGCATGAAGG1780.1115574803050909No Hit
AACGCCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAA1750.1096772980527579No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1740.10905057063531358No Hit
AACGCCGGGATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCACTGCCATCC1710.10717038838298058No Hit
AACGCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1690.10591693354809192No Hit
AACGCCGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG1680.1052902061306476No Hit
AACGCCGGGTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGA1650.1034100238783146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGC203.4290453E-452.02948845
AATCGCC203.4290453E-452.02948846
CGCCAAT203.4290453E-452.02948849
ACACGTA150.007192654552.02948452
CGTGCCA150.007192654552.02948446
CGTGACT150.007192654552.02948448
ACACACG150.007192654552.02948450
GCGTGAC150.007192654552.02948447
TAGGATA150.007192654552.02948447
CGGTATT150.007192654552.02948446
TACAGAT150.007192654552.02948449
GTAGGTC203.4343614E-452.01317225
TAACGTG203.4343614E-452.01317238
TATCTTG353.8475264E-852.01317224
CGCTATT203.4343614E-452.01317239
GCGCTAT203.4343614E-452.01317238
CTTCGAC150.007201583652.0131735
CGGAACT150.007201583652.0131724
TACCCGA150.007201583652.0131740
CGGATTG150.007201583652.0131740
Produced by FastQC (version 0.11.5)