FastQCFastQC Report
Tue 31 May 2016
SRR212735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159702
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7580.4746340058358693No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7310.45772751750134627No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5110.319970945886714No Hit
AACGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4440.2780178081677124No Hit
AACGCCGGGGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGG4410.2761393094638765No Hit
AACGCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4100.25672815619090555No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC3660.2291768418679791No Hit
AACGCCGGGATTCTTTACCCTGTAGACTTTTTTGAGGGGCGTATGCTCTGTAGCACTA3600.22541984446030733No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3540.22166284705263553No Hit
AACGCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3360.21039185482962017No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3310.20726102365656035No Hit
AACGCCGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGAAA3300.2066348574219484No Hit
AACGCCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACA3140.19661619766815694No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2880.18033587556824585No Hit
AACGCCGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT2740.17156954828367835No Hit
AACGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2600.16280322099911085No Hit
AACGCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT2600.16280322099911085No Hit
AACGCCGGGCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAG2550.15967238982605103No Hit
AACGCCGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGCA2540.15904622359143905No Hit
AACGCCGGGATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGGCT2430.15215839501070744No Hit
AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2410.1509060625414835No Hit
AACGCCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2370.14840139760303567No Hit
AACGCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.14527056642997582No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.13775657161463226No Hit
AACGCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2160.1352519066761844No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.1352519066761844No Hit
AACGCCGGGAGAAGAGGAAGTTCAGAACTCAGTTTCTGGGGAAAGTGAAGCATGAAGG2150.1346257404415724No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.12711174562622884No Hit
AACGCCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1980.12398091445316904No Hit
AACGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1960.1227285819839451No Hit
AACGCCGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTTAA1940.12147624951472118No Hit
AACGCCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA1940.12147624951472118No Hit
AACGCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC1880.11771925210704937No Hit
AACGCCGGGAGTTATCTAGGTTAACAACCCTCTCGAGTATTTGCTGTCTGTCAAGTTC1850.1158407534032135No Hit
AACGCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1750.10957909105709385No Hit
AACGCCGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACT1730.1083267585878699No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1680.10519592741481008No Hit
AACGCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1660.10394359494558617No Hit
AACGCCGGGGCTGAACCTTCATTAGACTCTTTCCTTGTACCCCTCCCCTACCCATTTC1630.10206509624175025No Hit
AACGCCGGGTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGA1600.10018659753791435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTT150.00711490152.17263825
ACCTATA150.00711490152.17263824
ATATCCC203.3828255E-452.17263824
ACATAAC150.00711490152.17263824
TATCCGT150.00711490152.17263824
CATATGG458.913048E-1152.1398523
CCAATAT203.3933474E-452.1398521
GCGGCTC203.3933474E-452.1398522
AACACTC203.3933474E-452.1398523
TTAGGCT150.0071326252.13984722
ACCGGCG150.0071326252.13984722
ATTAGAC353.774403E-852.13984721
ACTATAT203.435695E-452.0090952
TAATTTA251.6470205E-552.0090951
CCACGCA251.6470205E-552.0090952
ATCGAAC503.6379788E-1252.0090952
TCGTTCA150.007203822552.00908751
AGATCTT150.007203822552.00908752
GACCGTC150.007203822552.00908750
CAGGTTA150.0072127651.9927940
Produced by FastQC (version 0.11.5)