##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212735_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 159702 Sequences flagged as poor quality 0 Sequence length 58 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.173510663610976 39.0 38.0 40.0 35.0 40.0 2 38.06929155552216 39.0 38.0 40.0 35.0 40.0 3 37.71503174662809 39.0 38.0 40.0 33.0 40.0 4 37.24765500745138 39.0 36.0 40.0 33.0 40.0 5 37.147869156303614 39.0 36.0 39.0 33.0 40.0 6 38.169797497839724 39.0 38.0 40.0 35.0 40.0 7 38.0306570988466 39.0 38.0 40.0 35.0 40.0 8 38.10942254949844 39.0 38.0 40.0 35.0 40.0 9 38.084964496374496 39.0 38.0 40.0 35.0 40.0 10 36.91276878185621 39.0 36.0 40.0 31.0 40.0 11 37.31319582722821 39.0 37.0 40.0 33.0 40.0 12 37.211506430727226 39.0 36.0 40.0 33.0 40.0 13 37.13122565778763 39.0 36.0 40.0 32.0 40.0 14 37.16223967138796 39.0 36.0 40.0 33.0 40.0 15 37.16409312344241 39.0 36.0 40.0 33.0 40.0 16 36.999411403739465 39.0 36.0 40.0 33.0 40.0 17 36.72619002892888 38.0 36.0 40.0 31.0 40.0 18 36.61644187298844 38.0 36.0 40.0 31.0 40.0 19 36.66713002968028 38.0 36.0 40.0 31.0 40.0 20 36.574363502022514 38.0 36.0 40.0 31.0 40.0 21 36.53681231293284 38.0 36.0 40.0 31.0 40.0 22 36.446375123667835 38.0 35.0 40.0 31.0 40.0 23 36.29114225244518 38.0 35.0 40.0 30.0 40.0 24 36.145157856507744 38.0 35.0 40.0 30.0 40.0 25 36.14661682383439 38.0 35.0 40.0 30.0 40.0 26 35.763572153135215 38.0 35.0 39.0 29.0 40.0 27 35.252564150730734 38.0 35.0 39.0 28.0 40.0 28 35.429462373670965 38.0 35.0 39.0 29.0 40.0 29 35.391929969568324 38.0 35.0 39.0 28.0 40.0 30 35.1130417903345 38.0 34.0 39.0 28.0 40.0 31 34.74731061602234 38.0 33.0 39.0 27.0 40.0 32 34.6444315036756 38.0 33.0 39.0 27.0 40.0 33 34.364860803246046 38.0 33.0 39.0 26.0 40.0 34 34.11506430727229 38.0 33.0 39.0 25.0 40.0 35 33.926262664212096 37.0 33.0 39.0 24.0 40.0 36 34.62118195138445 38.0 34.0 39.0 27.0 40.0 37 34.36056530287661 38.0 33.0 39.0 26.0 40.0 38 34.20230804874078 38.0 33.0 39.0 25.0 40.0 39 34.22823133085372 38.0 33.0 39.0 26.0 40.0 40 34.05038759689923 38.0 33.0 39.0 25.0 40.0 41 33.99984345844135 38.0 33.0 39.0 25.0 40.0 42 33.86141062729333 38.0 33.0 39.0 24.0 40.0 43 33.724305268562695 38.0 33.0 39.0 23.0 40.0 44 33.58999887290078 38.0 33.0 39.0 23.0 40.0 45 33.39984471077381 37.0 33.0 39.0 23.0 40.0 46 33.02894140336377 37.0 33.0 39.0 20.0 40.0 47 32.921522585816085 37.0 32.0 39.0 20.0 40.0 48 32.74989668257129 36.0 32.0 39.0 19.0 40.0 49 32.57323640280021 36.0 32.0 39.0 18.0 40.0 50 32.324886350828415 36.0 32.0 39.0 16.0 40.0 51 32.00953025009079 36.0 32.0 39.0 2.0 40.0 52 31.90852337478554 36.0 31.0 39.0 2.0 40.0 53 31.636848630574445 36.0 31.0 39.0 2.0 40.0 54 31.40645076454897 36.0 31.0 39.0 2.0 40.0 55 31.24039147912988 36.0 31.0 39.0 2.0 40.0 56 30.829225682834277 36.0 30.0 39.0 2.0 40.0 57 30.598070155664924 36.0 30.0 39.0 2.0 40.0 58 30.31211882130468 35.0 30.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.36153802835898574 1 2 -0.9748808381405709 1 3 -1.2579031453608351 1 4 -0.8938991690013722 1 5 -1.6489760424217934 1 6 -1.641967932142336 1 7 -1.730938275068226 1 8 -1.3902895083462568 1 9 -1.322491477295607 1 10 -1.4107044676762612 1 11 -1.7620313606590372 1 12 -1.4749430698130652 1 13 -1.950144003632488 1 14 -1.3864656155223116 1 15 -1.9657971297404444 1 16 -1.4983145154398514 1 17 -1.4236252266980216 1 18 -1.1086791535580787 1 19 -1.2052122151375997 1 20 -1.5960606108123088 1 21 -1.4965820826924983 1 22 -1.208337199985749 1 23 -1.202107467004275 1 24 -1.7811879830541741 1 25 -1.385753835788499 1 26 -1.2997398615562474 1 27 -0.7353312969044126 1 28 -1.1801013012377624 1 29 -1.376707963639106 1 30 -1.2770174288640774 1 31 -0.9049079618426603 1 32 -0.9223681978118634 1 33 -0.6509310012845333 1 34 -0.9736016834503971 1 35 -1.2141740699737227 1 36 -1.5968044036293847 1 37 -1.1274425642593329 1 38 -0.8149563702475007 1 39 -1.1363135479622528 1 40 -1.4156777798930946 1 41 -0.19327236722691765 1 42 -0.5496759036732044 1 43 0.06771882904671145 1 44 -0.009689352572230803 1 45 -0.6847850781733342 1 46 -0.164347886647235 1 47 -0.6922245017150175 1 48 -0.803591099981741 1 49 -0.5384273239198052 1 50 -1.019675128761822 1 51 -0.5442011257236423 1 52 -0.4215036394136824 1 53 -1.0079747199699227 1 54 -0.7960894624756882 1 55 -0.92017967566386 1 56 -0.5798204082908569 1 57 -0.5468397976183539 1 58 -0.3124012465889834 2 1 -0.4834651574195661 2 2 -0.6306095475347746 2 3 -0.5218408099789187 2 4 -0.4286884578512442 2 5 -0.5529488255649042 2 6 -0.29648944223891505 2 7 -0.6176810318724364 2 8 -0.9819269095754066 2 9 -0.7747522323438929 2 10 -0.44757891894931134 2 11 -0.25489791026394926 2 12 0.08069784326858809 2 13 -0.14329589125321007 2 14 0.08741498149261417 2 15 -0.270340588915154 2 16 0.04624211318174787 2 17 0.37389452747230223 2 18 0.2370188271267324 2 19 0.41386153376495116 2 20 -0.029335413270601407 2 21 0.17966901476140862 2 22 0.08259783074296223 2 23 0.2820891967358534 2 24 -0.09462081887507168 2 25 3.304486715549615E-4 2 26 0.2903611042031855 2 27 0.3897784484862825 2 28 0.5528881632047344 2 29 0.43233505655404514 2 30 0.32866738237385107 2 31 0.07566710400458021 2 32 0.08125340008102455 2 33 -0.18352538232052495 2 34 0.25894879942932647 2 35 0.02507088173830141 2 36 -0.03973680046871664 2 37 0.09588930580212462 2 38 0.1851533751431944 2 39 0.21399373913168773 2 40 0.26507287857925377 2 41 0.3164949725448096 2 42 0.16718098833732853 2 43 -0.1569299857030657 2 44 -0.17266125775221042 2 45 0.24585583490831908 2 46 -0.1034391948122888 2 47 0.2016517054842808 2 48 0.4098636849172905 2 49 0.15274914666842676 2 50 -0.23027653350282407 2 51 -0.3298683776990572 2 52 0.380735166556466 2 53 0.29986110092560025 2 54 0.10689561215117749 2 55 0.36921891959513786 2 56 1.0567028577319704 2 57 0.6511408871928843 2 58 0.5698419491967961 3 1 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0.29821877720215895 117 29 0.18566266496966932 117 30 0.7270679834902083 117 31 0.14280965445541227 117 32 -0.2746396870808496 117 33 0.2676875846180735 117 34 0.38727640784495065 117 35 -0.05500640465070461 117 36 1.2035006404930684 117 37 0.7489056218175776 117 38 0.5830172652247754 117 39 0.6991805145717862 117 40 0.4812064122847062 117 41 0.9928045474696745 117 42 0.9484175705614604 117 43 0.634665119492297 117 44 1.1308488324152393 117 45 1.4747094201380335 117 46 0.5102898734573529 117 47 0.529313792216783 117 48 0.44491091548835726 117 49 0.5564631870291024 117 50 -0.22378232998200076 117 51 0.030425739948000796 117 52 0.40080815925719904 117 53 0.3591676702686648 117 54 0.6141948822241687 117 55 1.116589035524722 117 56 1.0358784695825527 117 57 1.3063577184509363 117 58 1.2494791325372852 118 1 -0.6632211023507324 118 2 -1.0998940698543436 118 3 -0.4716217861992007 118 4 -0.27375520795553854 118 5 -0.3886288422524302 118 6 -0.801912888213046 118 7 -0.7954031845633054 118 8 -0.7599306642687722 118 9 -0.9911164376004677 118 10 -0.27986928190300375 118 11 0.12729858535557526 118 12 -0.02114821430338054 118 13 -0.08447236184144202 118 14 0.09982632901034805 118 15 -0.3165963252522417 118 16 0.3010397769280999 118 17 0.15878188667130644 118 18 0.29949275286724486 118 19 0.4059871073986585 118 20 0.08664289294549832 118 21 -0.07888334235164507 118 22 0.19226616616223424 118 23 0.09205999356522199 118 24 -0.05915982596727076 118 25 0.08074346494188944 118 26 0.28457261859617944 118 27 0.7153938010102721 118 28 -0.5141226836872121 118 29 -0.26249181036293834 118 30 -0.1188190590112157 118 31 0.3096575086770841 118 32 0.08276570713150733 118 33 0.24258016628188983 118 34 -0.07506246465077737 118 35 0.6644117244583683 118 36 0.6029415474662017 118 37 0.5520324013382094 118 38 0.2811587985891677 118 39 0.5285536056314797 118 40 0.6980620316873072 118 41 0.4886372337045941 118 42 0.5370182849581084 118 43 0.12138666010419286 118 44 0.40112889660740336 118 45 -0.4082951872026541 118 46 0.8001381935898806 118 47 0.7367476587592066 118 48 0.7361569681880482 118 49 0.8587357966475722 118 50 0.4395711928217523 118 51 1.2431916973948063 118 52 1.251302542442989 118 53 0.9354993987979405 118 54 1.2877466759809622 118 55 1.106442532444536 118 56 1.5512794117578395 118 57 0.9477616631628472 118 58 1.5737530881204513 119 1 -0.6378769221254501 119 2 -0.9541389592994847 119 3 -0.08801728056715774 119 4 -0.6125119872630052 119 5 -0.9794194138001941 119 6 -0.392077677533031 119 7 -0.5441516201077334 119 8 -0.24663279192834864 119 9 -0.47328164410860296 119 10 0.2509504928153987 119 11 0.23039620738310873 119 12 -0.04430215673141191 119 13 0.09935116757032603 119 14 0.16094439325731713 119 15 0.20024764637896197 119 16 0.06094799553468988 119 17 -0.4128701784100457 119 18 -0.18209881993208654 119 19 -0.4170208191762299 119 20 -0.44845306032942744 119 21 -0.4820956911209393 119 22 -0.05710805160997978 119 23 -0.27673433267591463 119 24 -0.30785611299271665 119 25 -0.6761401395637421 119 26 -0.3199330134438725 119 27 0.09566080142745648 119 28 -0.29270007208938154 119 29 -0.7735472963871359 119 30 -0.141920852920272 119 31 -0.23315642540718784 119 32 -0.13933483521309142 119 33 -0.04381949996759005 119 34 0.3104193876646164 119 35 -0.06316441237934356 119 36 0.041145552472457325 119 37 0.10324224697859563 119 38 0.20721219867260743 119 39 0.37575844501403566 119 40 0.022425819755724774 119 41 0.36796556078010667 119 42 0.23335745892556758 119 43 0.2180700142969343 119 44 0.5111622716595505 119 45 0.7237970113789061 119 46 1.0656784522465301 119 47 0.8487105290136938 119 48 0.9319225084467035 119 49 1.0571609113743108 119 50 0.938841113556002 119 51 1.2068963281832978 119 52 1.189558695968234 119 53 1.4101552185726582 119 54 1.4083662003864745 119 55 1.4942189195951379 119 56 1.5567028577319704 119 57 1.1143761813105328 119 58 0.9521948903732671 120 1 -1.4006448199822685 120 2 -1.7543555575666971 120 3 -0.9459744169100688 120 4 -2.251818872807924 120 5 -2.4292028155329817 120 6 -1.2812135644459346 120 7 -2.0682054276772703 120 8 -1.927834342315947 120 9 -2.138238324455159 120 10 -1.9259190922279217 120 11 -1.2614528578243736 120 12 -1.3696555539042379 120 13 -1.4100537571902763 120 14 -1.2013447084298647 120 15 -1.2290159195125128 120 16 -1.4335800482409624 120 17 -1.4132121756740688 120 18 -1.5547048390839393 120 19 -1.0423058168962456 120 20 -1.5820029919299756 120 21 -1.185926060477989 120 22 -1.3394837926040495 120 23 -1.0596800691100228 120 24 -1.1721401987200295 120 25 -0.6203945855281745 120 26 -0.9596958953408148 120 27 -0.4526748118876327 120 28 -0.5926316726365783 120 29 -0.9276740633729972 120 30 -0.12920995460745388 120 31 -0.12115232144002164 120 32 -0.4435414015605659 120 33 0.12877511927545982 120 34 0.351459059347242 120 35 -0.22458712099767553 120 36 0.578366254706836 120 37 0.19478351464844934 120 38 0.6925063163196654 120 39 -0.43169709534164014 120 40 -0.05486556191322478 120 41 0.5600540240724001 120 42 -0.15999639424360623 120 43 0.5319095035810193 120 44 0.23767845953005207 120 45 0.7848435230068134 120 46 0.06271447595834445 120 47 0.7192645645814082 120 48 0.38187690881149905 120 49 0.39180523421973135 120 50 0.5586541550516699 120 51 0.5653094608782325 120 52 0.6578669495022034 120 53 0.4326130389100946 120 54 0.8549576276550539 120 55 0.9062116236535083 120 56 1.614614394439677 120 57 1.50887002535978 120 58 1.0134694001771862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113.0 3 13.0 4 22.0 5 34.0 6 75.0 7 109.0 8 113.0 9 194.0 10 224.0 11 232.0 12 308.0 13 403.0 14 478.0 15 536.0 16 571.0 17 606.0 18 686.0 19 721.0 20 752.0 21 871.0 22 1053.0 23 1198.0 24 1335.0 25 1477.0 26 1844.0 27 2105.0 28 2529.0 29 3029.0 30 3641.0 31 4418.0 32 5430.0 33 7030.0 34 8883.0 35 12064.0 36 16728.0 37 23662.0 38 28402.0 39 27702.0 40 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 39.608771336614446 48.02820252720692 3.1972047939286923 9.16582134224994 11 27.394772764273462 20.002254198444604 30.318342913676723 22.284630123605215 12 27.42044557989255 16.047388260635433 31.890646328787366 24.64151983068465 13 20.03043167900214 19.14691112196466 34.057181500544765 26.765475698488434 14 19.310340509198383 23.982166785638253 35.0690661356777 21.638426569485667 15 22.323452430151157 25.92891760904685 31.27700341886764 20.470626541934354 16 27.756696847879176 25.151219145658786 26.455523412355515 20.636560594106523 17 27.064783158632956 24.585164869569574 24.329062879613282 24.02098909218419 18 24.85046629463307 26.093709876427223 25.6465179784922 23.409305850447502 19 24.840170067814228 27.099391988779033 26.15575356447361 21.90468437893313 20 26.17124394184168 25.7642358893439 25.534432881241315 22.530087287573107 21 25.794291868605278 24.328436713378668 27.880051596097733 21.997219821918325 22 23.65405567870158 22.983431641432166 28.806777623323438 24.55573505654281 23 23.457439481033422 26.230729734129817 28.163704900376953 22.14812588445981 24 23.029327588474377 28.463354605176633 25.660230838118974 22.847086968230013 25 23.948353808969205 29.640831047826577 22.386069053612353 24.024746089591865 26 23.55217674465207 29.511923250338157 24.053779870747956 22.88212013426181 27 20.781365788713465 25.748269841867945 25.600060288631827 27.870304080786767 28 22.396948859579904 27.38072871654204 25.55392727864828 24.668395145229773 29 25.406140010270928 24.537495146360712 25.737440034069415 24.31892480929895 30 23.092106499902872 25.035247231917385 26.665079235777352 25.207567032402387 31 24.25994000613716 26.950896463618545 26.619615112441526 22.169548417802773 32 26.9841468685211 25.40572366319455 26.71982361747039 20.890305850813952 33 24.56101884972217 25.420501030501597 27.024199560235797 22.99428055954044 34 23.209531624873932 26.89664676735343 28.290433921960446 21.603387685812187 35 22.972405800732922 29.89945813887932 28.295799793278416 18.832336267109344 36 24.484668496171825 30.139217823891336 25.148803929676827 20.227309750260012 37 25.498452322769026 28.920260160156396 26.61002293319298 18.9712645838816 38 23.18875089293547 29.33277355156467 26.169588811048587 21.308886744451268 39 23.97071819395436 28.15929503049144 25.66419935695349 22.205787418600718 40 23.69524215328124 28.33215321858023 25.9291053894692 22.04349923866933 41 21.573125254614396 28.956159318103474 27.078436902635456 22.392278524646674 42 21.959427701216416 28.398729060520033 26.224720650259766 23.417122588003785 43 21.86238877052262 27.97092367464595 27.984083218448426 22.182604336383005 44 22.797859273556764 26.729000075201164 27.219687664502544 23.25345298673953 45 24.294872196564754 28.043438755240285 26.37469372920335 21.286995318991607 46 22.08929220510635 30.02456663700287 26.108318814785104 21.777822343105672 47 22.868372553690307 28.06752504293971 26.690320073216906 22.373782330153077 48 22.07756388960278 27.72843236195646 27.076529620830804 23.11747412760996 49 22.182793946157414 28.26547629496809 26.93508545347394 22.61664430540056 50 21.927574854992944 27.965511835710927 27.989967079479545 22.116946229816588 51 21.3654260921904 27.728595074484623 27.824522245071975 23.081456588252998 52 21.28402522662588 29.72779818699299 26.491091691011448 22.497084895369685 53 21.218619115692324 28.813984400471497 28.405186467032827 21.562210016803352 54 21.537786139855754 28.77140169332079 28.060206961429916 21.63060520539354 55 22.548527548088384 28.255768724042312 26.354191500307415 22.841512227561893 56 21.01487929011164 29.313017339299275 26.052877645921846 23.61922572466724 57 22.994145735422382 29.095004737373802 25.309497963870463 22.601351563333356 58 22.382777941610144 30.73969267836639 25.060705120563192 21.816824259460272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 162.5 12 325.0 13 948.0 14 1571.0 15 1927.5 16 2027.5 17 1771.0 18 1447.0 19 1123.0 20 1118.0 21 1113.0 22 953.5 23 779.5 24 765.0 25 920.0 26 1075.0 27 1257.5 28 1698.0 29 1956.0 30 2192.5 31 2429.0 32 2796.0 33 3163.0 34 2923.0 35 3315.0 36 3947.0 37 3811.5 38 3676.0 39 3723.0 40 3770.0 41 3783.0 42 3961.5 43 4127.0 44 4869.0 45 5611.0 46 5971.0 47 6668.5 48 7006.0 49 6892.0 50 6778.0 51 6832.5 52 6887.0 53 7409.0 54 8178.5 55 8426.0 56 8402.5 57 8379.0 58 8647.0 59 9903.5 60 10892.0 61 11531.0 62 12170.0 63 10466.5 64 8763.0 65 7641.0 66 5544.0 67 4569.0 68 3743.5 69 2918.0 70 2520.5 71 2123.0 72 1748.5 73 1174.0 74 974.0 75 808.5 76 643.0 77 459.5 78 230.5 79 185.0 80 141.5 81 98.0 82 84.0 83 70.0 84 55.0 85 25.0 86 10.0 87 6.0 88 2.0 89 1.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.02442048314986663 19 6.261662346119648E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.014401823396075191 25 0.0 26 0.008766327284567506 27 0.2930457977983995 28 0.015027989630687154 29 0.01753265456913501 30 0.07200911698037596 31 0.01189715845762733 32 0.030682145495986274 33 0.0444578026574495 34 0.04070080524977771 35 0.041953137719001644 36 0.060111958522748615 37 0.0676259533380922 38 0.07263528321498791 39 0.09329876895718275 40 0.07075678451115203 41 0.09204643648795882 42 0.08453244167261524 43 0.07639228062265971 44 0.08140161049955542 45 0.07576611438804774 46 0.08515860790722722 47 0.1102052572917058 48 0.10582209364942204 49 0.12523324692239296 50 0.142139735256916 51 0.12899024433006473 52 0.11771925210704937 53 0.1314949092685126 54 0.15779389112221512 55 0.19285920026048514 56 0.22103668081802355 57 0.20726102365656035 58 0.20350402624888853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 159702.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.718513230892537 #Duplication Level Percentage of deduplicated Percentage of total 1 62.034216076869 16.57462023017871 2 9.971877197093978 5.32867465654782 3 5.73002109210218 4.5929293308787615 4 3.925474572299039 4.195313771900164 5 2.9388329036794 3.9260622910170193 6 2.2474806655730024 3.6029605139572456 7 1.895945629247715 3.545979386607557 8 1.61237403327865 3.4464189553042544 9 1.3803609093039606 3.3193072096780254 >10 7.736114366065151 34.92066473807466 >50 0.3327865010546051 6.085709634193686 >100 0.1874853527068198 9.209026812438166 >500 0.007030700726505742 1.2523324692239295 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 758 0.4746340058358693 No Hit AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 731 0.45772751750134627 No Hit AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 511 0.319970945886714 No Hit AACGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 444 0.2780178081677124 No Hit AACGCCGGGGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGG 441 0.2761393094638765 No Hit AACGCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 410 0.25672815619090555 No Hit AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 366 0.2291768418679791 No Hit AACGCCGGGATTCTTTACCCTGTAGACTTTTTTGAGGGGCGTATGCTCTGTAGCACTA 360 0.22541984446030733 No Hit AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 354 0.22166284705263553 No Hit AACGCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 336 0.21039185482962017 No Hit AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 331 0.20726102365656035 No Hit AACGCCGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGAAA 330 0.2066348574219484 No Hit AACGCCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACA 314 0.19661619766815694 No Hit AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 288 0.18033587556824585 No Hit AACGCCGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT 274 0.17156954828367835 No Hit AACGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 260 0.16280322099911085 No Hit AACGCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 260 0.16280322099911085 No Hit AACGCCGGGCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAG 255 0.15967238982605103 No Hit AACGCCGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGCA 254 0.15904622359143905 No Hit AACGCCGGGATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGGCT 243 0.15215839501070744 No Hit AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 241 0.1509060625414835 No Hit AACGCCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC 237 0.14840139760303567 No Hit AACGCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 232 0.14527056642997582 No Hit AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.13775657161463226 No Hit AACGCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 216 0.1352519066761844 No Hit AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 216 0.1352519066761844 No Hit AACGCCGGGAGAAGAGGAAGTTCAGAACTCAGTTTCTGGGGAAAGTGAAGCATGAAGG 215 0.1346257404415724 No Hit AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 203 0.12711174562622884 No Hit AACGCCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 198 0.12398091445316904 No Hit AACGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 196 0.1227285819839451 No Hit AACGCCGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTTAA 194 0.12147624951472118 No Hit AACGCCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA 194 0.12147624951472118 No Hit AACGCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC 188 0.11771925210704937 No Hit AACGCCGGGAGTTATCTAGGTTAACAACCCTCTCGAGTATTTGCTGTCTGTCAAGTTC 185 0.1158407534032135 No Hit AACGCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 175 0.10957909105709385 No Hit AACGCCGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACT 173 0.1083267585878699 No Hit AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 168 0.10519592741481008 No Hit AACGCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG 166 0.10394359494558617 No Hit AACGCCGGGGCTGAACCTTCATTAGACTCTTTCCTTGTACCCCTCCCCTACCCATTTC 163 0.10206509624175025 No Hit AACGCCGGGTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGA 160 0.10018659753791435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 6.261662346119648E-4 0.0 0.0 0.0 0.0 11 0.0012523324692239296 0.0 0.0 0.0 0.0 12 0.0012523324692239296 0.0 0.0 0.0 0.0 13 0.0012523324692239296 0.0 0.0 0.0 0.0 14 0.002504664938447859 0.0 0.0 0.0 0.0 15 0.002504664938447859 0.0 0.0 0.0 0.0 16 0.003130831173059824 0.0 0.0 0.0 0.0 17 0.003130831173059824 0.0 0.0 0.0 0.0 18 0.003756997407671789 0.0 0.0 0.0 0.0 19 0.003756997407671789 0.0 0.0 0.0 0.0 20 0.005635496111507683 0.0 0.0 0.0 0.0 21 0.005635496111507683 0.0 0.0 0.0 0.0 22 0.008140161049955541 0.0 0.0 0.0 0.0 23 0.009392493519179472 0.0 0.0 0.0 0.0 24 0.012523324692239296 0.0 0.0 0.0 0.0 25 0.01314949092685126 0.0 0.0 0.0 0.0 26 0.01440182339607519 0.0 0.0 0.0 0.0 27 0.016280322099911083 0.0 0.0 0.0 0.0 28 0.02880364679215038 0.0 0.0 0.0 0.0 29 0.03130831173059824 0.0 0.0 0.0 0.0 30 0.03694380784210592 0.0 0.0 0.0 0.0 31 0.038196140311329854 0.0 0.0 0.0 0.0 32 0.040074639015165746 0.0 0.0 0.0 0.0 33 0.040074639015165746 0.0 0.0 0.0 0.0 34 0.04320547018822557 0.0 0.0 0.0 0.0 35 0.06386895593042041 0.0 0.0 0.0 0.0 36 0.06637362086886826 0.0 0.0 0.0 0.0 37 0.06699978710348023 0.0 0.0 0.0 0.0 38 0.06825211957270416 0.0 0.0 0.0 0.0 39 0.07200911698037595 0.0 0.0 0.0 0.0 40 0.07388761568421184 0.0 0.0 0.0 0.0 41 0.07451378191882381 0.0 0.0 0.0 0.0 42 0.07889694556110756 0.0 0.0 0.0 0.0 43 0.07889694556110756 0.0 0.0 0.0 0.0 44 0.08077544426494346 0.0 0.0 0.0 0.0 45 0.08265394296877936 0.0 0.0 0.0 0.0 46 0.08390627543800327 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCGTT 15 0.007114901 52.172638 25 ACCTATA 15 0.007114901 52.172638 24 ATATCCC 20 3.3828255E-4 52.172638 24 ACATAAC 15 0.007114901 52.172638 24 TATCCGT 15 0.007114901 52.172638 24 CATATGG 45 8.913048E-11 52.13985 23 CCAATAT 20 3.3933474E-4 52.13985 21 GCGGCTC 20 3.3933474E-4 52.13985 22 AACACTC 20 3.3933474E-4 52.13985 23 TTAGGCT 15 0.00713262 52.139847 22 ACCGGCG 15 0.00713262 52.139847 22 ATTAGAC 35 3.774403E-8 52.139847 21 ACTATAT 20 3.435695E-4 52.00909 52 TAATTTA 25 1.6470205E-5 52.00909 51 CCACGCA 25 1.6470205E-5 52.00909 52 ATCGAAC 50 3.6379788E-12 52.00909 52 TCGTTCA 15 0.0072038225 52.009087 51 AGATCTT 15 0.0072038225 52.009087 52 GACCGTC 15 0.0072038225 52.009087 50 CAGGTTA 15 0.00721276 51.99279 40 >>END_MODULE