FastQCFastQC Report
Tue 31 May 2016
SRR212734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158408
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6520.41159537397101154No Hit
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.40275743649310647No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.3086965304782587No Hit
AACGCCGGGGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGG3810.24051815564870463No Hit
AACGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC3790.23925559315186104No Hit
AACGCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3670.23168021817079948No Hit
AACGCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3400.21463562446341092No Hit
AACGCCGGGATTCTTTACCCTGTAGACTTTTTTGAGGGGCGTATGCTCTGTAGCACTA3290.20769153073077118No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3200.20200999949497503No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3000.18938437452653908No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC2880.1818089995454775No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2770.17486490581283776No Hit
AACGCCGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT2750.17360234331599414No Hit
AACGCCGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGAAA2750.17360234331599414No Hit
AACGCCGGGCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAG2680.16918337457704158No Hit
AACGCCGGGATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGGCT2600.1641331245896672No Hit
AACGCCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACA2560.16160799959598No Hit
AACGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2490.15718903085702743No Hit
AACGCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.14771981213070046No Hit
AACGCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2300.14519468713701328No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.1426695621433261No Hit
AACGCCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2090.13193778092015554No Hit
AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2030.12815009342962477No Hit
AACGCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT1940.1224685621938286No Hit
AACGCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1900.11994343720014142No Hit
AACGCCGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGCA1900.11994343720014142No Hit
AACGCCGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTTAA1890.11931215595171961No Hit
AACGCCGGGAGAAGAGGAAGTTCAGAACTCAGTTTCTGGGGAAAGTGAAGCATGAAGG1840.11615574970961062No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.10731781223170546No Hit
AACGCCGGGAGTTATCTAGGTTAACAACCCTCTCGAGTATTTGCTGTCTGTCAAGTTC1670.10542396848644009No Hit
AACGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1640.10353012474117469No Hit
AACGCCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1630.10289884349275288No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.10289884349275288No Hit
AACGCCGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACT1620.10226756224433109No Hit
AACGCCGGGGCTGAACCTTCATTAGACTCTTTCCTTGTACCCCTCCCCTACCCATTTC1600.10100499974748751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT203.3260207E-452.35157
CGGGATA3200.052.35156
GATAGTA203.3260207E-452.35159
CGGGTCG150.00701909552.3514986
CGGGTAT150.00701909552.3514986
CTCACGA150.00701909552.35149810
CGGGATG5550.052.3514986
GATCTAT150.00701909552.3514989
GACTCAC150.00701909552.3514989
GGAGCTA353.6552592E-852.3514948
GGACTAA503.6379788E-1252.3514948
GGATAGT353.6552592E-852.3514948
GGGACGC353.6552592E-852.3514947
CGGGCGA251.5841648E-552.3514946
CGGGCAT700.052.3514946
ATATACA203.4213284E-452.05291726
GTCGCTC150.0071797352.05291426
ATTAGTT150.0071797352.05291426
TGCGGGG150.0071797352.05291426
TCGCTCG150.0071797352.05291427
Produced by FastQC (version 0.11.5)