FastQCFastQC Report
Tue 31 May 2016
SRR212733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153768
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6980.4539305967431455No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6900.44872795380053065No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6660.4331200249726861No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.34337443421257996No Hit
AACGCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4170.27118776338379896No Hit
AACGCCGGGATTCTTTACCCTGTAGACTTTTTTGAGGGGCGTATGCTCTGTAGCAC3690.2399719057281099No Hit
AACGCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3330.21656001248634305No Hit
AACGCCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT3300.21460902138286247No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3250.21135736954372822No Hit
AACGCCGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCC3240.21070703917590136No Hit
AACGCCGGGGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCTGT3220.20940637844024762No Hit
AACGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA3120.2029030747619791No Hit
AACGCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3000.1950991103480568No Hit
AACGCCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGA2580.16778523489932887No Hit
AACGCCGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGA2550.1658342437958483No Hit
AACGCCGGGAGAAGAGGAAGTTCAGAACTCAGTTTCTGGGGAAAGTGAAGCATGAA2500.162582591956714No Hit
AACGCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.1567296186462723No Hit
AACGCCGGGCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGC2340.15217730607148433No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2240.14567400239321573No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.1437230112897352No Hit
AACGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2210.1437230112897352No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2110.13721970761146662No Hit
AACGCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC2100.13656937724363977No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.13201706466885177No Hit
AACGCCGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGG2010.13071640393319806No Hit
AACGCCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2010.13071640393319806No Hit
AACGCCGGGAGTTATCTAGGTTAACAACCCTCTCGAGTATTTGCTGTCTGTCAAGT1990.12941574319754434No Hit
AACGCCGGGACCTTTTGGGAGCTGACATCACACATGGGTGGCATATGTGGGATGTA1940.12616409135841009No Hit
AACGCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1930.1255137609905832No Hit
AACGCCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGT1920.12486343062275637No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.12486343062275637No Hit
AACGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1910.1242131002549295No Hit
AACGCCGGGATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGG1890.12291243951927579No Hit
AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA1870.12161177878362209No Hit
AACGCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1760.11445814473752666No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1710.11120649289839239No Hit
AACGCCGGGATGCATATTGTATGTATCTTGTTACCTACTCATCCAGCCGTGGGTAC1610.10470318922012381No Hit
AACGCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC1580.10275219811664325No Hit
AACGCCGGGGATGCACTCTCTACCTGATGCTTTTTTTTGTTGGTTTTAAGAAATGA1580.10275219811664325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA150.0081979450.3347650
ACGACTC150.0081979450.3347650
ACACGAC150.00820860550.31821448
ATTCGCC150.00821928150.30167447
TACACGA150.00821928150.30167447
AGCGTGG402.5265763E-950.2851542
CTACACG150.00822996750.2851546
CGACGCT204.0833215E-450.21916239
CACGACG204.0965667E-450.1862337
CATAACG150.008294350.1862336
ATAACGT150.008294350.1862337
AGTGCGG150.00832660750.13691334
ACTCACC150.00832660750.13691335
TAAGACT301.0223994E-650.13691334
ATAAGAC252.0495123E-550.120533
CTCCGCT150.00833739750.12049533
TCGAGTA204.1231592E-450.12049533
TACCTAC150.00834819750.1040932
CTCGAGT204.129829E-450.1040932
TAGGACT150.00836982950.07131231
Produced by FastQC (version 0.11.5)