FastQCFastQC Report
Tue 31 May 2016
SRR212732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138122
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6310.4568425015565949No Hit
AACGCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6040.4372945656738246No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5980.43295057992209784No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5510.3989226915335718No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.33376290525767077No Hit
AACGCCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT4290.3105949812484615No Hit
AACGCCGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCC3330.24109120922083377No Hit
AACGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA3100.2244392638392146No Hit
AACGCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3030.21937128046220009No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2930.2121313042093222No Hit
AACGCCGGGGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCTGT2920.21140730658403442No Hit
AACGCCGGGATTCTTTACCCTGTAGACTTTTTTGAGGGGCGTATGCTCTGTAGCAC2800.20271933508058093No Hit
AACGCCGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGA2570.1860673896989618No Hit
AACGCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2550.1846193944483862No Hit
AACGCCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGA2400.17375943006906938No Hit
AACGCCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2240.16217546806446473No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.15348749656101127No Hit
AACGCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2030.1469715179334212No Hit
AACGCCGGGAGAAGAGGAAGTTCAGAACTCAGTTTCTGGGGAAAGTGAAGCATGAA2010.1455235226828456No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1990.14407552743227No Hit
AACGCCGGGCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGC1990.14407552743227No Hit
AACGCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.14335152980698224No Hit
AACGCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1940.14045553930583107No Hit
AACGCCGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGG1900.1375595488046799No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.1375595488046799No Hit
AACGCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1890.13683555117939214No Hit
AACGCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1870.13538755592881654No Hit
AACGCCGGGGATGCACTCTCTACCTGATGCTTTTTTTTGTTGGTTTTAAGAAATGA1840.13321556305295318No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1800.13031957255180202No Hit
AACGCCGGGACCTTTTGGGAGCTGACATCACACATGGGTGGCATATGTGGGATGTA1800.13031957255180202No Hit
AACGCCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1780.12887157730122645No Hit
AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA1670.12090760342306076No Hit
AACGCCGGGATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGG1610.11656361767133404No Hit
AACGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1610.11656361767133404No Hit
AACGCCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGT1600.11583962004604625No Hit
AACGCCGGGAGTTATCTAGGTTAACAACCCTCTCGAGTATTTGCTGTCTGTCAAGT1600.11583962004604625No Hit
AACGCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC1580.11439162479547067No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1570.11366762717018289No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1520.11004763904374393No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.10715164854259278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACACG150.00824957550.2521950
AGCGACC150.00824957550.2521950
CGTTTGA204.0686442E-450.2521950
TAGGTGC150.00826148850.23385647
CGCTGAC204.0759813E-450.23385647
AACATCG252.0212112E-550.23385649
CGAATAG150.00826148850.23385648
ACTCGCT150.00826148850.23385646
GCGCTGA204.0759813E-450.23385646
TCGAATA150.00826148850.23385647
TAACCCC150.00827341350.21553444
GAACGAA150.00827341350.21553445
ATTTCGA150.00827341350.21553444
GGTATAG150.00829730350.17893643
CGAGGGT402.586603E-950.14238741
CGTGTTG150.00832124350.14238741
GGCGTAT355.142465E-850.12413438
AATATTG204.1202237E-450.12413439
CACTCAT150.00833323350.12413440
CTAGTCA150.00833323350.12413437
Produced by FastQC (version 0.11.5)