FastQCFastQC Report
Tue 31 May 2016
SRR212731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239976
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC12000.5000500050005001No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC8330.3471180451378471No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7790.3246157949128246No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6470.2696102943627696No Hit
TTCATAGGGGTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGGCT5170.21543821048771544No Hit
TTCATAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4580.19085241857519086No Hit
TTCATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4380.18251825182518253No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4030.16793346001266793No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3740.15584891822515584No Hit
TTCATAGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT3730.15543220988765544No Hit
TTCATAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG3640.1516818348501517No Hit
TTCATAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT3510.14626462646264626No Hit
TTCATAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3450.14376437643764378No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.1420975430876421No Hit
TTCATAGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG3400.14168083475014168No Hit
TTCATAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG3340.13918058472513917No Hit
TTCATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC3270.13626362636263628No Hit
TTCATAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3130.13042970963763043No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3080.12834616795012835No Hit
TTCATAGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT3020.12584591792512587No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGC2950.12292895956262292No Hit
TTCATAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2810.1170950428376171No Hit
TTCATAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG2680.11167783445011167No Hit
TTCATAGGGATTTGTAAAGATGTATAAGAGTAAGAAAACTAATGACTAAAAAATGCAA2640.11001100110011No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2590.10792745941260794No Hit
TTCATAGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAATG2490.10376037603760375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT150.007167201552.08418748
AGTCACG150.007179052652.06243542
TAGGTCA203.4218223E-452.06243541
CGCCCTA150.007179052652.06243542
CACAACG150.007202798552.01898639
CGTAGCG459.276846E-1152.01898240
GTACGGG353.8613507E-852.00813737
TCAATCG203.4395006E-452.00813337
TACCGTA459.276846E-1152.00813337
ACTATCC150.00722064651.98644630
CCTTACA150.00722064651.98644630
CGCGCAA150.00722064651.98644629
GTATCCG203.4465923E-451.98644623
TTCGACG150.00722064651.98644626
GTCGGCT150.00722064651.98644627
CCGGTAT203.4501424E-451.97561320
CGGTATC203.4501424E-451.97561321
CGGTAGG203.4501424E-451.97561331
CGAAAAG251.6558324E-551.9756119
CCCAATA150.007226602651.9756132
Produced by FastQC (version 0.11.5)