##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212731_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	239976
Sequences flagged as poor quality	0
Sequence length	58
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.781178117811784	39.0	38.0	40.0	33.0	40.0
2	37.503050305030506	39.0	38.0	40.0	33.0	40.0
3	36.76045104510451	39.0	35.0	40.0	31.0	40.0
4	36.827561922858955	39.0	36.0	40.0	31.0	40.0
5	37.04548788212155	39.0	36.0	40.0	31.0	40.0
6	37.19494032736607	39.0	36.0	40.0	33.0	40.0
7	37.00002916958363	39.0	36.0	40.0	32.0	40.0
8	37.73647364736474	39.0	38.0	40.0	34.0	40.0
9	37.687227056038935	39.0	38.0	40.0	34.0	40.0
10	36.49481198119812	38.0	35.0	40.0	30.0	40.0
11	37.06608577524419	39.0	36.0	40.0	33.0	40.0
12	36.88023802380238	39.0	35.0	40.0	31.0	40.0
13	36.80630563056306	38.0	35.0	40.0	31.0	40.0
14	36.78374087408741	38.0	35.0	40.0	31.0	40.0
15	36.80710987765443	38.0	35.0	40.0	31.0	40.0
16	36.4236131946528	38.0	35.0	40.0	30.0	40.0
17	36.10067256725672	38.0	35.0	39.0	30.0	40.0
18	36.046587992132544	38.0	35.0	39.0	29.0	40.0
19	36.08557939127246	38.0	35.0	39.0	29.0	40.0
20	35.90266109944328	38.0	35.0	39.0	29.0	40.0
21	35.782986631996536	38.0	35.0	39.0	29.0	40.0
22	35.656886521985534	38.0	35.0	39.0	28.0	40.0
23	35.51697669766977	38.0	34.0	39.0	28.0	40.0
24	35.44244841150782	38.0	34.0	39.0	28.0	40.0
25	35.339188085475215	38.0	33.0	39.0	28.0	40.0
26	34.89661049438277	38.0	33.0	39.0	26.0	40.0
27	34.79030819748642	38.0	33.0	39.0	26.0	40.0
28	34.53880804747141	38.0	33.0	39.0	26.0	40.0
29	34.51545571223789	38.0	33.0	39.0	26.0	40.0
30	34.290958262492914	38.0	33.0	39.0	26.0	40.0
31	33.84821815514885	37.0	33.0	39.0	23.0	40.0
32	33.70069506950695	37.0	32.0	39.0	23.0	40.0
33	33.524060739407275	37.0	32.0	39.0	23.0	40.0
34	33.24160332699937	36.0	31.0	39.0	23.0	40.0
35	33.031778177817785	36.0	31.0	39.0	22.0	40.0
36	33.99149081574824	38.0	33.0	39.0	24.0	40.0
37	33.89359352601927	38.0	33.0	39.0	24.0	40.0
38	33.7222430576391	38.0	33.0	39.0	23.0	40.0
39	33.68791045771244	38.0	33.0	39.0	23.0	40.0
40	33.47120128679535	37.0	32.0	39.0	23.0	40.0
41	33.627566923359005	38.0	33.0	39.0	23.0	40.0
42	33.486873687368735	38.0	33.0	39.0	23.0	40.0
43	33.3459262592926	37.0	32.0	39.0	23.0	40.0
44	33.244574457445744	37.0	32.0	39.0	22.0	40.0
45	32.97590592392572	37.0	32.0	39.0	21.0	40.0
46	32.67977214388105	37.0	32.0	39.0	18.0	40.0
47	32.497266393306	36.0	31.0	39.0	18.0	40.0
48	32.36862019535287	36.0	31.0	39.0	18.0	40.0
49	32.23969480281362	36.0	31.0	39.0	17.0	40.0
50	31.952420242024203	36.0	31.0	39.0	15.0	40.0
51	31.720467880121344	36.0	31.0	39.0	2.0	40.0
52	31.618015968263492	36.0	30.0	39.0	2.0	40.0
53	31.40700320032003	36.0	30.0	39.0	2.0	40.0
54	31.19016901690169	36.0	30.0	39.0	2.0	40.0
55	30.920575390872422	36.0	30.0	39.0	2.0	40.0
56	30.71635080174684	36.0	30.0	39.0	2.0	40.0
57	30.4274469113578	36.0	30.0	39.0	2.0	40.0
58	30.167291729172916	35.0	29.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-1.8108597350641489
1	2	-1.378977640539027
1	3	-3.767398302664972
1	4	-1.0661907077614714
1	5	-4.162989085438234
1	6	-2.131962766067403
1	7	-8.353467998025408
1	8	-3.187517172556383
1	9	-1.1084205860274636
1	10	-7.144710855007368
1	11	-4.126466726978933
1	12	-4.046385066343362
1	13	-0.9863750225973718
1	14	0.12574823998164675
1	15	-4.947767972727966
1	16	-15.9695373956771
1	17	-9.336694546204463
1	18	-12.190667066175372
1	19	-6.095056436950365
1	20	-11.06548035471986
1	21	-1.810974085025567
1	22	3.3779136179695755
1	23	3.4245388780922994
1	24	3.561718600581891
1	25	4.1705354609825385
1	26	-7.596671256922441
1	27	-7.675046915805147
1	28	-3.141962800050319
1	29	1.8249395768349892
1	30	-2.748886256831934
1	31	0.11437016140682488
1	32	5.024155541658121
1	33	2.5224136868782168
1	34	0.8771655163959053
1	35	3.3318021794512767
1	36	7.058683130004738
1	37	7.232892620683831
1	38	7.28686103796699
1	39	-11.354108482698347
1	40	1.8066000448156032
1	41	5.3020181323020665
1	42	8.710895315846052
1	43	8.978240659716153
1	44	8.893578339492556
1	45	3.3335346261190644
1	46	8.599515433239013
1	47	4.7609887107114375
1	48	1.0036588520887122
1	49	12.116869706701799
1	50	7.486617015549189
1	51	12.987402161031508
1	52	10.21974422604929
1	53	13.411213510020549
1	54	10.683136875475768
1	55	7.122084348097879
1	56	11.444050592677463
1	57	11.802336620815677
1	58	11.823873699784809
2	1	-2.810859735064149
2	2	-2.378977640539027
2	3	-11.767398302664972
2	4	-10.066190707761471
2	5	-14.162989085438234
2	6	-8.131962766067403
2	7	-10.353467998025408
2	8	-6.187517172556383
2	9	-4.108420586027464
2	10	-13.144710855007368
2	11	-1.1264667269789328
2	12	1.9536149336566382
2	13	2.0136249774026282
2	14	-0.8742517600183533
2	15	-3.9477679727279664
2	16	0.030462604322899267
2	17	-4.336694546204463
2	18	-23.190667066175372
2	19	-5.095056436950365
2	20	-10.06548035471986
2	21	-14.810974085025567
2	22	-14.622086382030425
2	23	-8.5754611219077
2	24	-19.43828139941811
2	25	-13.829464539017462
2	26	0.4033287430775587
2	27	-18.675046915805147
2	28	-1.1419628000503188
2	29	3.824939576834989
2	30	-26.748886256831934
2	31	-25.885629838593175
2	32	-25.97584445834188
2	33	-25.477586313121783
2	34	-25.122834483604095
2	35	-24.668197820548723
2	36	-25.941316869995262
2	37	-25.76710737931617
2	38	-25.71313896203301
2	39	-25.354108482698347
2	40	-25.193399955184397
2	41	-25.697981867697933
2	42	-25.289104684153948
2	43	-25.021759340283847
2	44	-25.106421660507444
2	45	-24.666465373880936
2	46	-24.400484566760987
2	47	-24.239011289288563
2	48	-23.996341147911288
2	49	-23.8831302932982
2	50	-23.51338298445081
2	51	-23.012597838968492
2	52	-22.78025577395071
2	53	-22.58878648997945
2	54	-22.316863124524232
2	55	-21.87791565190212
2	56	-21.555949407322537
2	57	-21.197663379184323
2	58	-21.17612630021519
3	1	-1.8108597350641489
3	2	-1.378977640539027
3	3	-12.767398302664972
3	4	-14.066190707761471
3	5	-13.162989085438234
3	6	-14.131962766067403
3	7	-14.853467998025408
3	8	-17.687517172556383
3	9	-15.108420586027464
3	10	-13.144710855007368
3	11	-14.126466726978933
3	12	-14.046385066343362
3	13	-13.986375022597372
3	14	-16.874251760018353
3	15	-13.947767972727966
3	16	-12.9695373956771
3	17	-13.836694546204463
3	18	-12.190667066175372
3	19	-12.095056436950365
3	20	-12.06548035471986
3	21	-12.810974085025567
3	22	-11.122086382030425
3	23	-11.0754611219077
3	24	-11.43828139941811
3	25	-10.329464539017462
3	26	-11.096671256922441
3	27	-9.675046915805147
3	28	-9.141962800050319
3	29	-10.67506042316501
3	30	-8.748886256831934
3	31	-12.385629838593175
3	32	-9.975844458341879
3	33	-9.477586313121783
3	34	-10.122834483604095
3	35	-8.668197820548723
3	36	-9.441316869995262
3	37	-9.267107379316169
3	38	-10.21313896203301
3	39	-9.854108482698347
3	40	-9.693399955184397
3	41	-8.197981867697933
3	42	-7.789104684153948
3	43	-8.021759340283847
3	44	-7.6064216605074435
3	45	-8.666465373880936
3	46	-6.400484566760987
3	47	-8.739011289288563
3	48	-8.496341147911288
3	49	-8.383130293298201
3	50	-7.013382984450811
3	51	-7.512597838968492
3	52	-7.28025577395071
3	53	-7.0887864899794515
3	54	-6.816863124524232
3	55	-5.377915651902121
3	56	-6.555949407322537
3	57	-11.697663379184323
3	58	-7.176126300215191
4	1	-2.310859735064149
4	2	-2.628977640539027
4	3	-6.767398302664972
4	4	-7.816190707761471
4	5	-7.912989085438234
4	6	-6.131962766067403
4	7	-7.603467998025408
4	8	-3.687517172556383
4	9	-5.108420586027464
4	10	-5.394710855007368
4	11	-7.126466726978933
4	12	-5.046385066343362
4	13	-7.986375022597372
4	14	-7.374251760018353
4	15	-3.9477679727279664
4	16	-7.219537395677101
4	17	-6.086694546204463
4	18	-9.940667066175372
4	19	-7.595056436950365
4	20	-8.56548035471986
4	21	0.9390259149744331
4	22	-5.8720863820304245
4	23	-5.825461121907701
4	24	0.3117186005818908
4	25	0.6705354609825385
4	26	-5.846671256922441
4	27	-8.175046915805147
4	28	-13.141962800050319
4	29	-14.67506042316501
4	30	-12.498886256831934
4	31	-15.635629838593175
4	32	-13.225844458341879
4	33	-13.227586313121783
4	34	-10.872834483604095
4	35	-14.168197820548723
4	36	-16.191316869995262
4	37	-13.517107379316169
4	38	-13.46313896203301
4	39	-12.854108482698347
4	40	-12.193399955184397
4	41	-12.447981867697933
4	42	-19.539104684153948
4	43	-18.021759340283847
4	44	-17.606421660507444
4	45	-22.416465373880936
4	46	-18.900484566760987
4	47	-19.489011289288563
4	48	-19.496341147911288
4	49	-18.3831302932982
4	50	-16.26338298445081
4	51	-23.012597838968492
4	52	-22.78025577395071
4	53	-22.58878648997945
4	54	-22.316863124524232
4	55	-21.87791565190212
4	56	-21.555949407322537
4	57	-21.197663379184323
4	58	-21.17612630021519
5	1	-9.810859735064149
5	2	-9.378977640539027
5	3	-8.43406496933164
5	4	-9.73285737442814
5	5	-7.162989085438234
5	6	-8.131962766067403
5	7	-10.353467998025408
5	8	-9.187517172556383
5	9	-9.108420586027464
5	10	-7.144710855007368
5	11	-10.126466726978933
5	12	-8.379718399676694
5	13	-7.65304168926404
5	14	-9.874251760018353
5	15	-10.614434639394634
5	16	-7.969537395677101
5	17	-9.336694546204463
5	18	-8.190667066175372
5	19	-9.095056436950365
5	20	-10.732147021386528
5	21	-8.810974085025567
5	22	-8.622086382030425
5	23	-8.908794455241033
5	24	-7.771614732751441
5	25	-9.162797872350794
5	26	-6.263337923589109
5	27	-8.00838024913848
5	28	-4.141962800050319
5	29	-7.508393756498343
5	30	-4.415552923498602
5	31	-3.2189631719265073
5	32	-3.9758444583418786
5	33	-5.144252979788451
5	34	-2.7895011502707625
5	35	-5.0015311538820555
5	36	-4.60798353666193
5	37	-1.767107379316169
5	38	-5.046472295366343
5	39	-4.020775149365015
5	40	-2.193399955184397
5	41	-2.0313152010312656
5	42	-4.289104684153948
5	43	-1.3550926736171789
5	44	-1.1064216605074435
5	45	-5.3331320405476035
5	46	-3.400484566760987
5	47	0.09432204404476963
5	48	-0.9963411479112878
5	49	-1.8831302932982013
5	50	-2.1800496511174785
5	51	-3.0125978389684924
5	52	-10.113589107284044
5	53	-8.922119823312785
5	54	-4.6501964578575645
5	55	-3.5445823185687892
5	56	-2.2226160739892045
5	57	-5.864330045850991
5	58	-2.5094596335485235
6	1	-0.8108597350641489
6	2	-0.37897764053902705
6	3	-2.517398302664972
6	4	-1.8161907077614714
6	5	-3.412989085438234
6	6	-2.381962766067403
6	7	-9.103467998025408
6	8	-2.937517172556383
6	9	-3.8584205860274636
6	10	-5.894710855007368
6	11	-6.626466726978933
6	12	-2.796385066343362
6	13	-1.4863750225973718
6	14	-4.874251760018353
6	15	-3.1977679727279664
6	16	-4.219537395677101
6	17	-5.086694546204463
6	18	-1.9406670661753722
6	19	-2.8450564369503653
6	20	-4.56548035471986
6	21	-1.560974085025567
6	22	0.6279136179695755
6	23	-2.3254611219077006
6	24	1.8117186005818908
6	25	-6.5794645390174615
6	26	-3.3466712569224413
6	27	-5.425046915805147
6	28	-5.641962800050319
6	29	-2.425060423165011
6	30	-0.7488862568319341
6	31	-2.885629838593175
6	32	-3.9758444583418786
6	33	1.7724136868782168
6	34	-6.122834483604095
6	35	-4.918197820548723
6	36	1.058683130004738
6	37	-1.517107379316169
6	38	-0.4631389620330104
6	39	1.1458915173016528
6	40	-1.1933999551843968
6	41	4.0520181323020665
6	42	0.46089531584605226
6	43	-0.27175934028384674
6	44	0.8935783394925565
6	45	3.8335346261190644
6	46	5.849515433239013
6	47	8.260988710711437
6	48	4.753658852088712
6	49	7.366869706701799
6	50	8.48661701554919
6	51	7.237402161031508
6	52	7.96974422604929
6	53	8.161213510020549
6	54	10.183136875475768
6	55	3.1220843480978786
6	56	3.6940505926774634
6	57	0.5523366208156766
6	58	3.8238736997848086
7	1	0.5224735982691868
7	2	0.9543556927943087
7	3	-0.10073163599830792
7	4	0.2671426255718643
7	5	-1.496322418771566
7	6	0.5347039005992613
7	7	-1.3534679980254083
7	8	0.14581616077695259
7	9	-0.44175391936079933
7	10	-3.4780441883407
7	11	-3.459800060312265
7	12	-2.7130517330100297
7	13	-4.319708355930704
7	14	-0.8742517600183533
7	15	-0.2811013060613021
7	16	-1.3028707290104329
7	17	3.6633054537955374
7	18	0.8093329338246278
7	19	-0.4283897702836974
7	20	2.267852978613476
7	21	5.189025914974433
7	22	3.711246951302911
7	23	3.4245388780922994
7	24	-4.771614732751441
7	25	-2.8294645390174615
7	26	-1.5966712569224413
7	27	1.6582864175281848
7	28	-7.808629466716987
7	29	0.49160624350165705
7	30	2.251113743168066
7	31	-11.885629838593175
7	32	-8.975844458341879
7	33	-13.477586313121783
7	34	-7.122834483604095
7	35	-8.334864487215391
7	36	-7.274650203328594
7	37	-5.767107379316169
7	38	-6.71313896203301
7	39	-5.687441816031679
7	40	-9.526733288517729
7	41	-5.364648534364601
7	42	-5.62243801748728
7	43	-4.021759340283847
7	44	-4.1064216605074435
7	45	-5.999798707214268
7	46	-4.067151233427655
7	47	-3.5723446226218947
7	48	-3.32967448124462
7	49	-3.549796959964869
7	50	-10.180049651117477
7	51	-5.012597838968492
7	52	-3.446922440617378
7	53	-1.5887864899794515
7	54	-3.6501964578575645
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120	16	-1.9695373956771007
120	17	0.6633054537955374
120	18	0.8093329338246278
120	19	-12.095056436950365
120	20	1.9345196452801403
120	21	-9.810974085025567
120	22	0.37791361796957545
120	23	0.4245388780922994
120	24	3.561718600581891
120	25	4.1705354609825385
120	26	-9.596671256922441
120	27	2.3249530841948527
120	28	1.8580371999496812
120	29	-1.1750604231650108
120	30	4.251113743168066
120	31	-5.885629838593175
120	32	-5.975844458341879
120	33	-19.477586313121783
120	34	-8.122834483604095
120	35	-20.668197820548723
120	36	0.058683130004737905
120	37	-11.767107379316169
120	38	-2.7131389620330104
120	39	0.6458915173016528
120	40	2.806600044815603
120	41	0.30201813230206653
120	42	3.7108953158460523
120	43	-1.0217593402838467
120	44	-9.106421660507444
120	45	-0.6664653738809356
120	46	-0.4004845667609871
120	47	5.7609887107114375
120	48	6.003658852088712
120	49	2.1168697067017987
120	50	6.486617015549189
120	51	-23.012597838968492
120	52	-22.78025577395071
120	53	-22.58878648997945
120	54	-22.316863124524232
120	55	-21.87791565190212
120	56	-21.555949407322537
120	57	-21.197663379184323
120	58	-21.17612630021519
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132.0
3	8.0
4	25.0
5	63.0
6	86.0
7	172.0
8	235.0
9	352.0
10	378.0
11	508.0
12	667.0
13	818.0
14	914.0
15	1001.0
16	1014.0
17	1109.0
18	1146.0
19	1205.0
20	1342.0
21	1541.0
22	1756.0
23	2119.0
24	2486.0
25	2978.0
26	3542.0
27	4175.0
28	4826.0
29	5874.0
30	6676.0
31	7554.0
32	8956.0
33	10779.0
34	13531.0
35	17322.0
36	23545.0
37	32475.0
38	36317.0
39	42254.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	45.73207320732073	46.28254492115878	2.173967396739674	5.811414474780811
11	26.21762176217622	21.513818048471514	29.627546087942125	22.641014101410143
12	24.803730373037304	17.4021568823549	30.83808380838084	26.956028936226957
13	20.65914924825816	18.076390972430577	34.67680101343468	26.587658765876586
14	20.3011967863453	21.00876754342101	35.74899156582325	22.94104410441044
15	21.91135780244691	24.01781844851152	31.96111277794446	22.109710971097112
16	28.422425575890927	25.083758375837583	25.915508217488416	20.57830783078308
17	27.4061881445984	24.102927388269613	24.809250963642047	23.681633503489945
18	24.564539787312064	24.046154615461546	27.140214021402144	24.24909157582425
19	25.70143892386997	24.780494309729093	25.544753324360027	23.973313442040915
20	26.49403469585908	24.56838534656271	26.74656520996287	22.191014747615338
21	27.405623226333404	22.379140638999203	28.26781792648217	21.947418208185223
22	26.661943952495054	20.64340035420356	28.77758099802063	23.917074695280757
23	25.19346087652258	23.11946026811573	28.16215292681199	23.5249259285497
24	24.542973226377747	23.061152203354514	27.13532659652047	25.260547973747265
25	26.143973330555266	27.506615272424213	23.543285758933223	22.806125638087302
26	25.081376206055804	28.232646341717548	23.63515118677975	23.05082626544689
27	23.198606475056987	24.71256464430526	25.841886592739836	26.246942287897916
28	24.62656708455588	25.082938783675644	26.098209522539346	24.192284609229127
29	26.81756801813817	23.66506068284876	25.204221125633502	24.31315017337957
30	25.344250403345157	22.900416477327205	26.551159174382683	25.204173944944948
31	25.58331492127281	25.465755651807786	25.37445962339346	23.576469803525946
32	27.649358552220267	24.645932495852886	25.165259288280556	22.53944966364629
33	27.24829026168061	23.650442385673625	25.338717810868143	23.762549541777627
34	25.927160874603	25.875478272467344	26.47649691988363	21.720863933046022
35	25.5092471020745	27.058558727538735	26.847648939407204	20.584545230979565
36	25.43525389810723	28.28358208955224	24.71775202201284	21.56341199032769
37	27.23349788199193	27.389013041593007	23.763800406924386	21.613688669490678
38	25.717240459033896	28.778856418468106	23.47711502535362	22.026788097144383
39	26.0108738252683	26.64337355425655	23.437929970563953	23.907822649911193
40	24.755880954962016	27.318401280843226	24.634551079460646	23.291166684734115
41	24.024918065597557	27.66547413541484	25.253308648770357	23.056299150217242
42	23.868190898815953	27.012023972874115	24.807420413644685	24.312364714665243
43	24.2974634447438	26.09707474538524	25.767180761884106	23.838281047986854
44	24.830597173631123	25.025749229608067	24.440293061677224	25.703360535083586
45	24.880847639261276	25.049725000938206	25.721898598526387	24.347528761274127
46	25.18775202138351	27.075905609000422	24.24075626222317	23.495586107392903
47	23.839918565891956	26.897452263843174	25.70556063128121	23.557068538983657
48	24.76346615939127	25.563592978240557	26.096315578694433	23.576625283673742
49	24.874920195120325	27.185401861905216	24.218120819371826	23.721557123602633
50	23.220642425810546	26.3501416808904	26.027551612324363	24.401664280974696
51	22.5656129928536	25.84302944084505	27.043691932866366	24.54766563343499
52	23.41457717191352	26.291968915159075	26.435045947332636	23.858407965594772
53	23.54305934586849	26.379326172771506	26.601320280747437	23.47629420061257
54	23.994558072956877	26.594914469099116	26.010241172518267	23.400286285425732
55	24.228534215008516	26.85435661089403	24.404284807801492	24.512824366295963
56	24.42580445828758	26.6520256023782	23.670509250174316	25.251660689159905
57	24.124398278230302	26.34508038961093	24.5172657094785	25.013255622680262
58	24.011590765800563	27.476294462236066	24.63455267410157	23.877562097861805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	71.5
7	143.0
8	441.5
9	884.5
10	1029.0
11	932.0
12	835.0
13	705.0
14	575.0
15	584.0
16	546.5
17	500.0
18	547.0
19	594.0
20	718.5
21	843.0
22	1031.0
23	1514.0
24	1809.0
25	2231.0
26	2653.0
27	3050.5
28	3829.0
29	4210.0
30	4794.0
31	5378.0
32	5841.0
33	6304.0
34	6326.0
35	6925.5
36	7503.0
37	7378.5
38	7254.0
39	7900.0
40	8546.0
41	9337.5
42	10416.5
43	10704.0
44	11360.5
45	12017.0
46	11711.5
47	11718.5
48	12031.0
49	12460.5
50	12890.0
51	12849.5
52	12809.0
53	12688.0
54	13660.0
55	14753.0
56	16371.5
57	17990.0
58	16323.0
59	13123.5
60	11591.0
61	10364.0
62	9137.0
63	7532.5
64	5928.0
65	4922.5
66	3175.5
67	2434.0
68	2181.5
69	1929.0
70	1543.0
71	1157.0
72	883.0
73	478.0
74	347.0
75	268.0
76	189.0
77	164.5
78	106.0
79	72.0
80	52.0
81	32.0
82	22.0
83	12.0
84	7.5
85	1.5
86	0.0
87	1.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	4.1670833750041673E-4
18	0.0
19	0.0020835416875020834
20	0.0020835416875020834
21	0.002916958362502917
22	4.1670833750041673E-4
23	0.0020835416875020834
24	4.1670833750041673E-4
25	4.1670833750041673E-4
26	0.017085041837517086
27	0.0037503750375037508
28	0.016668333500016667
29	0.016668333500016667
30	0.04458779211254459
31	0.04042070873754042
32	0.020835416875020836
33	0.011251125112511251
34	0.020835416875020836
35	0.026252625262526252
36	0.048338167150048336
37	0.05333866720005334
38	0.07000700070007
39	0.05583891722505584
40	0.05583891722505584
41	0.06250625062506251
42	0.08542520918758542
43	0.08417508417508417
44	0.06792345901256792
45	0.06542320898756543
46	0.06875687568756876
47	0.11376137613761376
48	0.11001100110011
49	0.13709704303763712
50	0.1470980431376471
51	0.11459479281261459
52	0.1020935426876021
53	0.13751375137513752
54	0.14793145981264794
55	0.18001800180018002
56	0.1946027936126946
57	0.1896022935626896
58	0.19793646031269793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	239976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.75660899423276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.3007836352534	18.769793646031268
2	14.475625890494607	9.483448344834484
3	8.466059434154284	8.319581958195819
4	5.410390799918583	7.089042237557089
5	3.7044575615713415	6.067273394006067
6	2.5455424384286585	5.003000300030003
7	1.7339202116832892	3.9758142480914755
8	1.2683187461835945	3.3236656999033234
9	0.9528292285772442	2.809030903090309
>10	3.7896906167311215	20.887088708870888
>50	0.22135151638510076	5.045504550455045
>100	0.12466924486057397	7.569923659032569
>500	0.00508854060655404	1.1567823449011567
>1k	0.00127213515163851	0.5000500050005001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	1200	0.5000500050005001	No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	833	0.3471180451378471	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	779	0.3246157949128246	No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	647	0.2696102943627696	No Hit
TTCATAGGGGTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGGCT	517	0.21543821048771544	No Hit
TTCATAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	458	0.19085241857519086	No Hit
TTCATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	438	0.18251825182518253	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	403	0.16793346001266793	No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	374	0.15584891822515584	No Hit
TTCATAGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT	373	0.15543220988765544	No Hit
TTCATAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	364	0.1516818348501517	No Hit
TTCATAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	351	0.14626462646264626	No Hit
TTCATAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA	345	0.14376437643764378	No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	341	0.1420975430876421	No Hit
TTCATAGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG	340	0.14168083475014168	No Hit
TTCATAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG	334	0.13918058472513917	No Hit
TTCATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC	327	0.13626362636263628	No Hit
TTCATAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	313	0.13042970963763043	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	308	0.12834616795012835	No Hit
TTCATAGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT	302	0.12584591792512587	No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGC	295	0.12292895956262292	No Hit
TTCATAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	281	0.1170950428376171	No Hit
TTCATAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG	268	0.11167783445011167	No Hit
TTCATAGGGATTTGTAAAGATGTATAAGAGTAAGAAAACTAATGACTAAAAAATGCAA	264	0.11001100110011	No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	259	0.10792745941260794	No Hit
TTCATAGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAATG	249	0.10376037603760375	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	4.1670833750041673E-4	0.0	0.0	0.0	0.0
16	4.1670833750041673E-4	0.0	0.0	0.0	0.0
17	4.1670833750041673E-4	0.0	0.0	0.0	0.0
18	4.1670833750041673E-4	0.0	0.0	0.0	0.0
19	4.1670833750041673E-4	0.0	0.0	0.0	0.0
20	4.1670833750041673E-4	0.0	0.0	0.0	0.0
21	4.1670833750041673E-4	0.0	0.0	0.0	0.0
22	4.1670833750041673E-4	0.0	0.0	0.0	0.0
23	8.334166750008335E-4	0.0	0.0	0.0	0.0
24	8.334166750008335E-4	0.0	0.0	0.0	0.0
25	0.001666833350001667	0.0	0.0	0.0	0.0
26	0.001666833350001667	0.0	0.0	0.0	0.0
27	0.001666833350001667	0.0	0.0	0.0	0.0
28	0.0025002500250025004	0.0	0.0	0.0	0.0
29	0.002916958362502917	0.0	0.0	0.0	0.0
30	0.003333666700003334	0.0	0.0	0.0	0.0
31	0.004583791712504583	0.0	0.0	0.0	0.0
32	0.005000500050005001	0.0	0.0	0.0	0.0
33	0.005833916725005834	0.0	0.0	0.0	0.0
34	0.00625062506250625	0.0	0.0	0.0	0.0
35	0.006667333400006668	0.0	0.0	0.0	0.0
36	0.007917458412507917	0.0	0.0	0.0	0.0
37	0.00875087508750875	0.0	0.0	0.0	0.0
38	0.010001000100010001	0.0	0.0	0.0	0.0
39	0.010417708437510418	0.0	0.0	0.0	0.0
40	0.0125012501250125	0.0	0.0	0.0	0.0
41	0.014168083475014168	0.0	0.0	0.0	0.0
42	0.014584791812514585	0.0	0.0	0.0	0.0
43	0.015418208487515418	0.0	0.0	0.0	0.0
44	0.015418208487515418	0.0	0.0	0.0	0.0
45	0.015418208487515418	0.0	0.0	0.0	0.0
46	0.015834916825015834	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACTT	15	0.0071672015	52.084187	48
AGTCACG	15	0.0071790526	52.062435	42
TAGGTCA	20	3.4218223E-4	52.062435	41
CGCCCTA	15	0.0071790526	52.062435	42
CACAACG	15	0.0072027985	52.018986	39
CGTAGCG	45	9.276846E-11	52.018982	40
GTACGGG	35	3.8613507E-8	52.008137	37
TCAATCG	20	3.4395006E-4	52.008133	37
TACCGTA	45	9.276846E-11	52.008133	37
ACTATCC	15	0.007220646	51.986446	30
CCTTACA	15	0.007220646	51.986446	30
CGCGCAA	15	0.007220646	51.986446	29
GTATCCG	20	3.4465923E-4	51.986446	23
TTCGACG	15	0.007220646	51.986446	26
GTCGGCT	15	0.007220646	51.986446	27
CCGGTAT	20	3.4501424E-4	51.975613	20
CGGTATC	20	3.4501424E-4	51.975613	21
CGGTAGG	20	3.4501424E-4	51.975613	31
CGAAAAG	25	1.6558324E-5	51.97561	19
CCCAATA	15	0.0072266026	51.97561	32
>>END_MODULE