FastQCFastQC Report
Tue 31 May 2016
SRR212730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256952
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC12280.4779102711790529No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC8990.34987079298857376No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8660.33702792739499987No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6620.2576356673619976No Hit
TTCATAGGGGTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGGCT5510.2144369376381581No Hit
TTCATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4950.19264298390360846No Hit
TTCATAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4440.1727949188953579No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.17201656340483826No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4230.16462218624490177No Hit
TTCATAGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT4080.15878452006600455No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3990.1552819203586662No Hit
TTCATAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT3850.1498334319250288No Hit
TTCATAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG3830.1490550764345092No Hit
TTCATAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG3780.14710918770821008No Hit
TTCATAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3780.14710918770821008No Hit
TTCATAGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT3690.14360658800087175No Hit
TTCATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC3560.13854727731249417No Hit
TTCATAGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG3330.1295961891715184No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGC3250.12648276720943988No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA3090.12025592328528285No Hit
TTCATAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT3050.1186992123042436No Hit
TTCATAGGGATTTGTAAAGATGTATAAGAGTAAGAAAACTAATGACTAAAAAATGCAA3010.11714250132320433No Hit
TTCATAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3000.11675332357794452No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2950.11480743485164545No Hit
TTCATAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG2710.10546716896540989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCATA150.00716229552.0942651
TCGAACT203.4119663E-452.0942652
TACGTCT203.425139E-452.05362744
CATGACG150.007184434752.05362346
CGAATTC150.007184434752.05362345
CTCGAAT150.007184434752.05362343
AACGTAG150.007189977452.0434848
CGTAGCG503.6379788E-1252.03333340
ACGCCGG203.438352E-452.01305827
ATACCGG203.438352E-452.01305827
CGGCACA150.00720662552.01305431
GTCGTCT203.451606E-451.9725522
AATTCGT203.451606E-451.9725513
GAATTCG203.451606E-451.9725512
ACTAGCG150.007228866751.97254622
GTCGTAG150.007228866751.97254614
CGTCGTA150.007228866751.97254613
GACGCAC353.9017323E-851.9422079
ATCTCGA150.00724558151.94220410
GGACGGA150.00724558151.9422048
Produced by FastQC (version 0.11.5)