FastQCFastQC Report
Tue 31 May 2016
SRR212729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266526
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC11170.4190960731786017No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC8080.3031599168561416No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7610.28552561476178684No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6250.23449869806322834No Hit
TTCATAGGGGTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGGCT4990.1872237605336815No Hit
TTCATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4840.18159579178016405No Hit
TTCATAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4400.16508708343651277No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4040.1515799584280708No Hit
TTCATAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG3880.14557679175765217No Hit
TTCATAGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT3820.1433256042562452No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.14144961467173933No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3700.13882322925343119No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGC3610.1354464480013207No Hit
TTCATAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG3490.13094407299850672No Hit
TTCATAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT3300.12381531257738457No Hit
TTCATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC3260.12231452090977991No Hit
TTCATAGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT3160.11856254174076825No Hit
TTCATAGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG3150.1181873438238671No Hit
TTCATAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3140.11781214590696593No Hit
TTCATAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3110.11668655215626242No Hit
TTCATAGGGATTTGTAAAGATGTATAAGAGTAAGAAAACTAATGACTAAAAAATGCAA3050.11443536465485543No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3010.11293457298725078No Hit
TTCATAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2860.1073066042337333No Hit
TTCATAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG2690.10092823964641348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA203.3462848E-452.300429
ATCCCGT251.5963438E-552.3004210
GACGCAC307.660965E-752.3004159
TGTAGCG251.6415686E-552.0542342
GTACGTC203.4251672E-452.0542343
CAAGCGG150.007184387752.05422639
TCAAGCG150.007184387752.05422638
GCGGGTA251.6507398E-552.0052729
CGCATCG150.00721117652.00526428
GTCGAAA150.0072326651.96616748
ACGACTT308.0076643E-751.96616748
GTTACGA203.4699828E-451.91737411
ACGCGGA203.4732017E-451.90762336
TACGGAT150.00727037251.89787722
CGAGCCT150.00727037251.89787732
ATAGGGG125950.051.855744
TAGGGGT27150.051.7225075
ATAGGGA120000.051.6827054
TAGGGAC20800.051.6718065
TAGGGGC34950.051.4773755
Produced by FastQC (version 0.11.5)