FastQCFastQC Report
Tue 31 May 2016
SRR212728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502439
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC22900.4557767211542098No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC15430.3071019566554348No Hit
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG13950.27764564454590507No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13040.2595339931812618No Hit
TTCATAGGGGTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGG12200.24281554576774494No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA10180.2026116603209544No Hit
TTCATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8860.17633981438542787No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8600.17116505685267266No Hit
TTCATAGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG8040.16001942524366142No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7770.154645638575031No Hit
TTCATAGGGATTTGTAAAGATGTATAAGAGTAAGAAAACTAATGACTAAAAAATGC7590.1510631141292774No Hit
TTCATAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC7210.14350000696601975No Hit
TTCATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC7160.14250486128664375No Hit
TTCATAGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGG6860.13653398721038773No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC6240.12419418078612528No Hit
TTCATAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5290.10528641287798121No Hit
TTCATAGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAA5290.10528641287798121No Hit
TTCATAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5260.10468932547035562No Hit
TTCATAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5060.10070874275285159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTC150.00827169350.23770542
ATTACGA150.00831444550.17223437
CACGTCG150.008393850.05180429
CACTACG150.00840708150.0317927
TCGGCGT150.00842370450.00679426
CGATTAG1150.049.94690324
CTAGTCG150.0084770649.9269823
GGGTACG252.1338585E-549.8077288
GGGTCGA204.2618733E-449.8077248
TCGACTA355.4442353E-849.80772414
TACGTCA150.00855756649.80772411
TTCATAG507950.049.2635081
ATAGGGA248200.049.2458344
TCATAGG508150.049.244122
CATAGGG508300.049.229593
ATAGGGG223750.049.195564
TAGGGAT99900.049.1346445
TAGGGAG68400.048.9703145
TAGGGGC55150.048.9497455
TAGGGAC41050.048.8977175
Produced by FastQC (version 0.11.5)