FastQCFastQC Report
Tue 31 May 2016
SRR212727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470119
Sequences flagged as poor quality0
Sequence length56
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC21200.4509496531729201No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC15580.33140545266198557No Hit
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG13550.28822489624967296No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC11680.24844773344621257No Hit
TTCATAGGGGTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGG10870.23121805330139816No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA10380.22079516037428823No Hit
TTCATAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC10190.2167536304637762No Hit
TTCATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8710.1852722395818931No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8330.17718917976086906No Hit
TTCATAGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG7280.15485440920277632No Hit
TTCATAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC7090.1508128792922643No Hit
TTCATAGGGATTTGTAAAGATGTATAAGAGTAAGAAAACTAATGACTAAAAAATGC6730.1431552436723468No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6390.1359230322535358No Hit
TTCATAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA6320.13443404754966296No Hit
TTCATAGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGG6200.1318815023430238No Hit
TTCATAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5620.11954420051093446No Hit
TTCATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC5510.11720436740484856No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC5140.10933401968437778No Hit
TTCATAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG5110.10869588338271799No Hit
TTCATAGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAA4980.10593062607552556No Hit
TTCATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT4940.10507977767331252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA150.00828667550.2142145
CGCTTAC150.00828667550.2142144
GCGCGTA150.00833592650.1389936
TCGTTGA150.008438637549.98389426
GTATCGC204.2012337E-449.9519224
GGTATCG204.203444E-449.94659423
CCGAAAA204.2456161E-449.8456518
GATCGTA150.008535108549.840359
ATTCCGA204.2478452E-449.8403510
ATAGGGA225850.049.2665864
TTCATAG475950.049.2277531
TCATAGG476100.049.2122462
TAGGGAT88600.049.193445
CATAGGG476500.049.1709333
ATAGGGG214450.049.1663674
TAGGGGC56100.049.0851975
TAGGGAC40300.048.6036225
TAGGGAG64500.048.5653655
TAGGGGA50200.048.5496675
AGGGATT32300.048.5287676
Produced by FastQC (version 0.11.5)