##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212727_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 470119 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.58551345510392 40.0 38.0 40.0 36.0 40.0 2 38.551213628889705 40.0 38.0 40.0 36.0 40.0 3 38.390760637200366 39.0 38.0 40.0 35.0 40.0 4 38.32243325626065 39.0 38.0 40.0 35.0 40.0 5 38.40271505725146 39.0 38.0 40.0 35.0 40.0 6 38.40126223360468 39.0 38.0 40.0 35.0 40.0 7 38.730917065679115 39.0 38.0 40.0 38.0 40.0 8 38.73507984148694 39.0 38.0 40.0 37.0 40.0 9 38.72961952186574 40.0 38.0 40.0 37.0 40.0 10 37.84173368870435 39.0 38.0 40.0 33.0 40.0 11 37.99003656521008 39.0 38.0 40.0 35.0 40.0 12 38.014994075968 39.0 38.0 40.0 35.0 40.0 13 38.031409068767694 39.0 38.0 40.0 35.0 40.0 14 37.9886603179195 39.0 38.0 40.0 35.0 40.0 15 38.00141878971069 39.0 38.0 40.0 35.0 40.0 16 37.87771819475495 39.0 38.0 40.0 35.0 40.0 17 37.685239269206306 39.0 38.0 40.0 33.0 40.0 18 37.618375347518395 39.0 38.0 40.0 33.0 40.0 19 37.705030003041784 39.0 38.0 40.0 33.0 40.0 20 37.52360147111689 39.0 38.0 40.0 33.0 40.0 21 37.37790857208494 39.0 38.0 40.0 33.0 40.0 22 37.32868699201691 39.0 37.0 40.0 33.0 40.0 23 37.09367840908366 39.0 37.0 40.0 33.0 40.0 24 37.00039564450703 39.0 37.0 40.0 33.0 40.0 25 36.939072873038526 39.0 36.0 40.0 33.0 40.0 26 36.4487140489961 38.0 36.0 40.0 31.0 40.0 27 36.32875505988909 38.0 36.0 40.0 31.0 40.0 28 36.085491120333366 38.0 35.0 40.0 30.0 40.0 29 35.990836362708166 38.0 35.0 40.0 30.0 40.0 30 35.756642467120024 38.0 35.0 40.0 29.0 40.0 31 35.2538633835263 38.0 35.0 40.0 28.0 40.0 32 35.11652581580408 38.0 35.0 40.0 28.0 40.0 33 34.91258383515663 38.0 34.0 39.0 27.0 40.0 34 35.254159053346065 38.0 35.0 40.0 28.0 40.0 35 34.983989160191356 38.0 35.0 40.0 28.0 40.0 36 34.945675456639705 38.0 35.0 40.0 28.0 40.0 37 34.7351798161742 38.0 35.0 40.0 27.0 40.0 38 34.607622750835425 38.0 34.0 40.0 27.0 40.0 39 34.492911369249065 38.0 34.0 40.0 26.0 40.0 40 34.20416320123203 38.0 34.0 40.0 25.0 40.0 41 33.675422605765775 38.0 33.0 39.0 23.0 40.0 42 33.521497748442414 38.0 33.0 39.0 23.0 40.0 43 33.309155766944116 37.0 33.0 39.0 22.0 40.0 44 33.14549507677843 37.0 33.0 39.0 20.0 40.0 45 32.82117719130688 37.0 32.0 39.0 18.0 40.0 46 32.180543649586596 36.0 32.0 39.0 8.0 40.0 47 31.950225368470537 36.0 31.0 39.0 2.0 40.0 48 31.7895809358907 36.0 31.0 39.0 2.0 40.0 49 31.579051261489113 36.0 31.0 39.0 2.0 40.0 50 31.192062009831552 36.0 31.0 39.0 2.0 40.0 51 30.656465703364468 36.0 30.0 39.0 2.0 40.0 52 30.484528385366257 36.0 30.0 39.0 2.0 40.0 53 30.197290473263152 35.0 30.0 38.0 2.0 40.0 54 29.87875835692665 35.0 30.0 38.0 2.0 40.0 55 29.469434334711 35.0 29.0 38.0 2.0 40.0 56 16.935150461904325 20.0 15.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.3454366641328832 1 2 -0.40010135795952095 1 3 -0.3672055778360601 1 4 -0.5695184705791263 1 5 -0.5971302722849856 1 6 -0.5173050821722782 1 7 -0.6604221951662055 1 8 -0.5270919909426937 1 9 -0.5535441834786923 1 10 -0.5278570998606327 1 11 -0.463120832941847 1 12 -0.5115094901931982 1 13 -0.6159292851348397 1 14 -0.6560153397527984 1 15 -0.34540961393106073 1 16 -0.6544507164391504 1 17 -0.5762370397482997 1 18 -0.6468416686334777 1 19 -0.6029914130402148 1 20 -0.8009043315573336 1 21 -0.3115398854668072 1 22 -0.6687729308760666 1 23 -0.46711420951476157 1 24 -0.30710979211653466 1 25 -0.4835026488612755 1 26 -0.5994588060545496 1 27 -0.22416828920565735 1 28 -0.6077631249139515 1 29 -0.5050035599686211 1 30 -0.21002208550773815 1 31 -0.8036038444691869 1 32 -0.5393459726936882 1 33 -0.6075545487991789 1 34 -0.7440721313586778 1 35 -0.6886639524163414 1 36 -1.090158750169202 1 37 -0.494083149463755 1 38 -1.0161824730072269 1 39 -1.0335534506545372 1 40 -0.603144788986782 1 41 -0.23650698789532498 1 42 -0.35556998680062435 1 43 -0.4224216246972503 1 44 -0.07981414159549871 1 45 -0.2820478300709439 1 46 0.027190854258101638 1 47 -0.2801255930060087 1 48 -0.253687773164863 1 49 -0.2874982332349454 1 50 -0.035067025719040856 1 51 -0.03394473797733255 1 52 0.24539912743198045 1 53 0.293467993113282 1 54 -0.08815165275822423 1 55 0.10588088535520157 1 56 0.34442677596057436 2 1 -0.28868244274451627 2 2 -0.358942821374157 2 3 -0.4347595177985326 2 4 -0.5130456750819405 2 5 -0.6496044749116265 2 6 -0.5900654011216204 2 7 -0.3176571857853432 2 8 -0.37383214103650886 2 9 -0.27723086265316965 2 10 -0.6934879629000363 2 11 -0.826699632191378 2 12 -0.7953744057654291 2 13 -0.7589639943280844 2 14 -0.5022160902218431 2 15 -0.5376352236871611 2 16 -0.5057874894222678 2 17 -0.5987511110428585 2 18 -0.5258050832676275 2 19 -0.5008924449351468 2 20 -0.5948302227393185 2 21 -0.2740398854668058 2 22 -0.017612049074934077 2 23 -0.18258090745096922 2 24 -0.3528884037488069 2 25 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120 54 1.685715047348367 120 55 2.237682252212057 120 56 1.913396617329937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102.0 3 25.0 4 17.0 5 75.0 6 106.0 7 184.0 8 182.0 9 300.0 10 472.0 11 517.0 12 683.0 13 1326.0 14 1933.0 15 1573.0 16 1587.0 17 2049.0 18 2395.0 19 2193.0 20 2672.0 21 2884.0 22 2996.0 23 3625.0 24 4393.0 25 4804.0 26 5098.0 27 5929.0 28 6854.0 29 7636.0 30 9173.0 31 10657.0 32 13220.0 33 17096.0 34 23091.0 35 33361.0 36 49947.0 37 74017.0 38 98472.0 39 78475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 44.68464367532476 47.704091942678346 2.074155692494879 5.53710868950202 11 26.061911983987034 20.38845483803037 32.29267483339325 21.25695834458935 12 24.56548235659482 16.927628962028763 32.507726767052596 25.99916191432382 13 20.800052752600937 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24.25632847382059 29.36893048845662 24.342134679546824 22.03260635817597 27 22.726130599764062 25.58845700122227 26.184686276931462 25.500726122082202 28 24.32104892111154 25.77617989780857 26.500976547940304 23.401794633139588 29 25.991950621387318 24.637319885033225 25.863902009787942 23.50682748379152 30 25.150259796469665 23.86481628754363 26.899435206525936 24.08548870946077 31 25.20846039998806 25.63625739474616 25.80089312752445 23.354389077741335 32 26.886208897155434 24.92897121307783 25.767878777536986 22.41694111222975 33 26.82627064396364 24.47403234754406 25.97512055648018 22.72457645201212 34 25.534802145609564 26.14758960309637 27.254959695142507 21.062648556151558 35 24.577731007234018 27.071108540154242 27.858760547386126 20.49239990522561 36 25.314552065042317 28.176815643651498 25.38718208345616 21.121450207850025 37 26.157374290820652 27.633919324910373 24.765386282544313 21.443320101724662 38 25.060475254295238 28.631079579451235 24.641636037268142 21.666809128985385 39 25.321399407436544 26.619823086032863 24.721995391173113 23.336782115357483 40 24.64854412129613 27.41441418315921 25.216050174979248 22.72099152056541 41 23.9969274484128 27.62707440025334 26.03687917768592 22.33911897364795 42 23.52810234523323 26.88082054267631 25.767599951198942 23.823477160891517 43 23.929461327031262 26.52499850154555 26.055768193377688 23.4897719780455 44 24.36292337099961 25.618346950701348 25.077005734492364 24.941723943806686 45 24.493064566551467 25.44997880185516 26.12360873791813 23.933347893675244 46 24.650461589856373 26.890205643581105 24.85689750799297 23.602435258569553 47 23.043595644292456 27.135963728149683 26.09631924713626 23.7241213804216 48 23.82247658242771 25.82084753392065 26.624011317600154 23.732664566051486 49 24.30330608257494 27.511054552933313 24.84425077553259 23.34138858895916 50 22.88679330222109 26.886081593541455 26.530012883642062 23.697112220595393 51 22.517325470262765 26.277713252216845 27.161880108174362 24.043081169346024 52 23.006130365141615 27.111695295764925 26.344702776997515 23.537471562095945 53 22.859109653816216 27.29968926450689 26.550621578210343 23.290579503466553 54 23.58784322808101 26.974874975338185 26.36540655532395 23.07187524125685 55 23.73712667353244 27.141048746996223 24.861611740473737 24.260212838997596 56 23.509460677049812 26.784227914360493 24.362852361951344 25.34345904663835 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 159.0 7 318.0 8 919.5 9 1521.0 10 1984.0 11 2447.0 12 2193.5 13 1705.5 14 1471.0 15 1462.0 16 1453.0 17 1387.0 18 1321.0 19 1570.0 20 1819.0 21 2101.0 22 3151.0 23 3919.0 24 4661.0 25 5403.0 26 6656.0 27 7909.0 28 8820.5 29 10536.0 30 11340.0 31 12252.0 32 13164.0 33 14314.5 34 15465.0 35 15815.0 36 16165.0 37 15940.0 38 16761.5 39 17808.0 40 20322.5 41 22837.0 42 22966.0 43 23095.0 44 22358.0 45 21621.0 46 22093.0 47 24035.0 48 25505.0 49 25104.5 50 24704.0 51 24452.5 52 24201.0 53 24096.5 54 26988.0 55 29984.0 56 29764.5 57 29545.0 58 28490.0 59 27435.0 60 24296.0 61 21157.0 62 17520.5 63 12337.5 64 10791.0 65 8509.5 66 6228.0 67 5159.5 68 4091.0 69 3635.0 70 3179.0 71 2453.5 72 1353.5 73 979.0 74 772.5 75 566.0 76 476.5 77 387.0 78 300.5 79 144.5 80 75.0 81 59.0 82 43.0 83 27.0 84 11.0 85 7.5 86 4.0 87 4.0 88 2.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.001276272603319585 23 0.00510509041327834 24 4.2542420110652833E-4 25 0.028077997273030873 26 0.09699671785228846 27 0.10656876237718535 28 0.12932895713638462 29 0.16293746902380035 30 0.2335578864074841 31 0.2563180811666833 32 0.27546217021647706 33 0.3103469547072124 34 0.30651813689725366 35 0.34842242070624674 36 0.4239352164026555 37 0.38203093259366244 38 0.45924542509449734 39 0.49370478538412615 40 0.5604963849578511 41 0.5860218370242428 42 0.6202684852133183 43 0.6319676507437479 44 0.6266498482299163 45 0.6587693754134591 46 0.6691922683405691 47 0.7276880959927168 48 0.7634237288856651 49 0.7110965521495621 50 0.7734211976116685 51 0.7370474284170604 52 0.7980958012758471 53 0.8095822547057233 54 0.8106458152084897 55 0.8591441741346341 56 0.8463814481014381 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 470119.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.772851840140362 #Duplication Level Percentage of deduplicated Percentage of total 1 55.29406713704271 13.144976656876903 2 11.514441267090412 5.474622125290767 3 6.876789439297047 4.904426894287519 4 5.118311685405376 4.8670746147520445 5 3.703167731431478 4.40174289092546 6 2.9631735397131327 4.226585132173475 7 2.3977563122903147 3.990105389258728 8 1.9723965797037941 3.7511593327438315 9 1.535381131929325 3.2850349350752555 >10 8.010721778101706 32.386782267723376 >50 0.38945370574518157 6.375300425230603 >100 0.20728987563856435 9.525511614100614 >500 0.01076830522797737 1.673129705038769 >1k 0.006281511382986799 1.9935480165226696 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2120 0.4509496531729201 No Hit TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1558 0.33140545266198557 No Hit TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1355 0.28822489624967296 No Hit TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1168 0.24844773344621257 No Hit TTCATAGGGGTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGG 1087 0.23121805330139816 No Hit TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 1038 0.22079516037428823 No Hit TTCATAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 1019 0.2167536304637762 No Hit TTCATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 871 0.1852722395818931 No Hit TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 833 0.17718917976086906 No Hit TTCATAGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG 728 0.15485440920277632 No Hit TTCATAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 709 0.1508128792922643 No Hit TTCATAGGGATTTGTAAAGATGTATAAGAGTAAGAAAACTAATGACTAAAAAATGC 673 0.1431552436723468 No Hit TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 639 0.1359230322535358 No Hit TTCATAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 632 0.13443404754966296 No Hit TTCATAGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGG 620 0.1318815023430238 No Hit TTCATAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 562 0.11954420051093446 No Hit TTCATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC 551 0.11720436740484856 No Hit TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 514 0.10933401968437778 No Hit TTCATAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 511 0.10869588338271799 No Hit TTCATAGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAA 498 0.10593062607552556 No Hit TTCATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 494 0.10507977767331252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 2.1271210055326417E-4 0.0 0.0 0.0 0.0 11 6.381363016597925E-4 0.0 0.0 0.0 0.0 12 6.381363016597925E-4 0.0 0.0 0.0 0.0 13 6.381363016597925E-4 0.0 0.0 0.0 0.0 14 8.508484022130567E-4 0.0 0.0 0.0 0.0 15 8.508484022130567E-4 0.0 0.0 0.0 0.0 16 0.001276272603319585 0.0 0.0 0.0 0.0 17 0.001276272603319585 0.0 0.0 0.0 0.0 18 0.0014889847038728492 0.0 0.0 0.0 0.0 19 0.0017016968044261133 0.0 0.0 0.0 0.0 20 0.0019144089049793775 0.0 0.0 0.0 0.0 21 0.0021271210055326417 0.0 0.0 0.0 0.0 22 0.0027652573071924344 0.0 0.0 0.0 0.0 23 0.0027652573071924344 0.0 0.0 0.0 0.0 24 0.0031906815082989627 0.0 0.0 0.0 0.0 25 0.004466954111618547 0.0 0.0 0.0 0.0 26 0.00510509041327834 0.0 0.0 0.0 0.0 27 0.005955938815491397 0.0 0.0 0.0 0.0 28 0.005955938815491397 0.0 0.0 0.0 0.0 29 0.007870347720470775 0.0 0.0 0.0 0.0 30 0.008508484022130567 0.0 0.0 0.0 0.0 31 0.009784756625450152 0.0 0.0 0.0 0.0 32 0.010848317128216474 0.0 0.0 0.0 0.0 33 0.011273741329323001 0.0 0.0 0.0 0.0 34 0.011486453429876265 0.0 0.0 0.0 0.0 35 0.012124589731536057 0.0 0.0 0.0 0.0 36 0.013188150234302379 0.0 0.0 0.0 0.0 37 0.0142517107370687 0.0 0.0 0.0 0.0 38 0.015102559139281756 0.0 0.0 0.0 0.0 39 0.015953407541494814 0.0 0.0 0.0 0.0 40 0.018080528547027453 0.0 0.0 0.0 0.0 41 0.02042036165311336 0.0 0.0 0.0 0.0 42 0.021058497954773152 0.0 0.0 0.0 0.0 43 0.021483922155879683 0.0 0.0 0.0 0.0 44 0.022122058457539475 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGA 15 0.008286675 50.21421 45 CGCTTAC 15 0.008286675 50.21421 44 GCGCGTA 15 0.008335926 50.13899 36 TCGTTGA 15 0.0084386375 49.983894 26 GTATCGC 20 4.2012337E-4 49.95192 24 GGTATCG 20 4.203444E-4 49.946594 23 CCGAAAA 20 4.2456161E-4 49.84565 18 GATCGTA 15 0.0085351085 49.84035 9 ATTCCGA 20 4.2478452E-4 49.84035 10 ATAGGGA 22585 0.0 49.266586 4 TTCATAG 47595 0.0 49.227753 1 TCATAGG 47610 0.0 49.212246 2 TAGGGAT 8860 0.0 49.19344 5 CATAGGG 47650 0.0 49.170933 3 ATAGGGG 21445 0.0 49.166367 4 TAGGGGC 5610 0.0 49.085197 5 TAGGGAC 4030 0.0 48.603622 5 TAGGGAG 6450 0.0 48.565365 5 TAGGGGA 5020 0.0 48.549667 5 AGGGATT 3230 0.0 48.528767 6 >>END_MODULE