FastQCFastQC Report
Tue 31 May 2016
SRR212726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86513
Sequences flagged as poor quality0
Sequence length58
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12631.4598962005710125No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9351.0807624287679307No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGGGTCCATCCTGACAG3930.45426698877625327No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.43692855409013676No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3730.43114907586143125No Hit
ATGGCGGGGAGCCCAGCTGTCTTGTCTCCCTCTGGCTCTGCTGGGATCCTAGGCCCTT3620.4184342237582791No Hit
ATGGCGGGGTGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTTTT2880.3328979459734375No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.30862413741287437No Hit
ATGGCGGGGTGGCATCCTTTCTACGGTATCCTTGCTTTGACAACTGCAGTAGACTCAA2310.2670118941661947No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.21615248575358614No Hit
ATGGCGGGGATTTGATTTTGCTTTTGCGTGATAACTTTACTTCCCAATGACTTCTTTG1830.21152890317062178No Hit
ATGGCGGGGAAGTATTACAAATGCTGATGTGCTATGGTTAGTGAATTGTCAAGAATAA1810.20921711187913955No Hit
ATGGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.20574942494191623No Hit
ATGGCGGGGGGTAAGCAGTCATTGCTCCTGACTTTGGTCACTCAGCAAACCGTTTGTC1600.1849433033185764No Hit
ATGGCGGGGATTTCTATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCACTG1520.1756961381526476No Hit
ATGGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.16529307734097765No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGTGTCCATCCTGACAG1430.16529307734097765No Hit
ATGGCGGGGATCCTGATGACTACCACACATACTGGCTATTTATATATAAATATATGTG1410.16298128604949547No Hit
ATGGCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGC1310.15142232959208443No Hit
ATGGCGGGGTACAGGACTTGGCTTATCTGTATCACTGCCTTTTTATACAAGCCAGATG1160.1340838949059679No Hit
ATGGCGGGGATAAACAGTAGATGTTTGAGATTGAGAGATGGGAATTACTGTCATCTAA1130.1306162079687446No Hit
ATGGCGGGGGTTTAGGCCCAAGAAGGAAGCTTTGGTGGTGGTGTCTTGACACTTGGGT1110.1283044166772624No Hit
ATGGCGGGGATTTAGTGTTTTCTTGGTCACAATTTTCTTTGCTGTCTAGCATGATCTG1100.12714852103152127No Hit
ATGGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA1100.12714852103152127No Hit
ATGGCGGGGGGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTTTT1060.12252493844855687No Hit
ATGGCGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.11674546021985135No Hit
ATGGCGGGGGCTCTTCGGACTTGGGCTTGGGGTCCGGCGACATGGCTAAACGCACCAA970.11212187763688694No Hit
ATGGCGGGGCACAAGGTAGGAGGAGTGAGCCTCATCTGGTGGGTCATCCTCAGATCTT930.10749829505392254No Hit
ATGGCGGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.10518650376244033No Hit
ATGGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.10171881682521702No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1005629211794759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACAG550.052.11130552
TAGTTCG150.00713703852.11130552
TATTCGT150.00713703852.11130550
TCATCTA251.6217062E-552.11130551
CGCATAG203.393787E-452.111350
AACCGTT353.79805E-852.0509648
CGTTCAG150.00716984252.05095741
TAAGGTG150.00716984252.05095741
ATATTCG150.00716984252.05095749
ACGCATA203.413277E-452.05095349
GCCGGAA203.413277E-452.05095348
ACTAGGG203.413277E-452.05095342
ACAGGTA203.413277E-452.05095347
TACTGTC251.6328813E-552.05095346
CACAGGT203.413277E-452.05095346
GACGCAT203.413277E-452.05095348
CTAGAGG150.007186285752.02083631
TAGAGGT150.007186285752.02083632
TGATAAC251.6384929E-552.02083629
ACCGGAA150.007186285752.02083629
Produced by FastQC (version 0.11.5)