FastQCFastQC Report
Tue 31 May 2016
SRR212725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84886
Sequences flagged as poor quality0
Sequence length58
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11711.3794972080201684No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9361.1026553259665905No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.46415192140046646No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.45354946634309545No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGGGTCCATCCTGACAG3600.4240982022948425No Hit
ATGGCGGGGAGCCCAGCTGTCTTGTCTCCCTCTGGCTCTGCTGGGATCCTAGGCCCTT3340.3934688876846594No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.31453950003534153No Hit
ATGGCGGGGTGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTTTT2480.2921565393586693No Hit
ATGGCGGGGTGGCATCCTTTCTACGGTATCCTTGCTTTGACAACTGCAGTAGACTCAA2370.279197983177438No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.20615884833777065No Hit
ATGGCGGGGAAGTATTACAAATGCTGATGTGCTATGGTTAGTGAATTGTCAAGAATAA1610.189666140470749No Hit
ATGGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.18024173597530807No Hit
ATGGCGGGGATTTGATTTTGCTTTTGCGTGATAACTTTACTTCCCAATGACTTCTTTG1500.17670758428951772No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGTGTCCATCCTGACAG1490.1755295337275876No Hit
ATGGCGGGGATTTCTATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCACTG1460.17199538204179723No Hit
ATGGCGGGGATCCTGATGACTACCACACATACTGGCTATTTATATATAAATATATGTG1410.16610512923214665No Hit
ATGGCGGGGGGTAAGCAGTCATTGCTCCTGACTTTGGTCACTCAGCAAACCGTTTGTC1390.16374902810828643No Hit
ATGGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.14490021911740453No Hit
ATGGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA1200.14136606743161417No Hit
ATGGCGGGGTACAGGACTTGGCTTATCTGTATCACTGCCTTTTTATACAAGCCAGATG1190.14018801686968405No Hit
ATGGCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGC1180.13900996630775395No Hit
ATGGCGGGGGTTTAGGCCCAAGAAGGAAGCTTTGGTGGTGGTGTCTTGACACTTGGGT1060.1248733595645925No Hit
ATGGCGGGGGCTCTTCGGACTTGGGCTTGGGGTCCGGCGACATGGCTAAACGCACCAA1040.12251725844073227No Hit
ATGGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.11898310675494193No Hit
ATGGCGGGGATAAACAGTAGATGTTTGAGATTGAGAGATGGGAATTACTGTCATCTAA1010.11898310675494193No Hit
ATGGCGGGGATTTAGTGTTTTCTTGGTCACAATTTTCTTTGCTGTCTAGCATGATCTG970.11427090450722144No Hit
ATGGCGGGGGGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTTTT920.10838065169757087No Hit
ATGGCGGGGCACAAGGTAGGAGGAGTGAGCCTCATCTGGTGGGTCATCCTCAGATCTT910.10720260113564073No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA900.10602455057371063No Hit
ATGGCGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT870.10249039888792026No Hit
ATGGCGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.10013429776406003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGG150.007146923352.092251
TAGTTCG150.007146923352.092252
TTGACGA150.007146923352.092251
GAGCCGA150.007146923352.092252
TTTGACG150.007146923352.092250
AATAGTT150.007146923352.092250
CGTTTGT150.007146923352.092251
GTAGACT550.052.06143649
AGTAGAC550.052.06143648
TTAGGCT150.007163654552.0614340
CGGTTTG203.409503E-452.0614346
CTCCAAA150.007163654552.0614349
ATCCGGT203.409503E-452.0614343
AATTGTC307.842609E-752.0614344
AAACCGT150.007163654552.0614347
TATTATC150.007163654552.0614344
ATCCAAC150.007163654552.0614343
CGGTGTC203.409503E-452.0614341
ACCGTTT150.007163654552.0614349
TAGGCTA150.007163654552.0614341
Produced by FastQC (version 0.11.5)