FastQCFastQC Report
Tue 31 May 2016
SRR212724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89979
Sequences flagged as poor quality0
Sequence length58
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10771.1969459540559464No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9031.0035674990831194No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3740.4156525411484902No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.3989819846853155No Hit
ATGGCGGGGAGCCCAGCTGTCTTGTCTCCCTCTGGCTCTGCTGGGATCCTAGGCCCTT3370.37453183520599254No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGGGTCCATCCTGACAG3130.3478589448649129No Hit
ATGGCGGGGTGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTTTT2750.3056268684915369No Hit
ATGGCGGGGTGGCATCCTTTCTACGGTATCCTTGCTTTGACAACTGCAGTAGACTCAA2740.3045154980606586No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.2422787539314729No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGTGTCCATCCTGACAG1830.20338078885073185No Hit
ATGGCGGGGATTTCTATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCACTG1750.19448982540370532No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.19004434368019205No Hit
ATGGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.1878216028184354No Hit
ATGGCGGGGATTTGATTTTGCTTTTGCGTGATAACTTTACTTCCCAATGACTTCTTTG1680.1867102323875571No Hit
ATGGCGGGGGGTAAGCAGTCATTGCTCCTGACTTTGGTCACTCAGCAAACCGTTTGTC1640.18226475066404382No Hit
ATGGCGGGGAAGTATTACAAATGCTGATGTGCTATGGTTAGTGAATTGTCAAGAATAA1530.17003967592438235No Hit
ATGGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.1455895264450594No Hit
ATGGCGGGGATCCTGATGACTACCACACATACTGGCTATTTATATATAAATATATGTG1240.13780993342891118No Hit
ATGGCGGGGTACAGGACTTGGCTTATCTGTATCACTGCCTTTTTATACAAGCCAGATG1200.13336445170539793No Hit
ATGGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA1120.1244734882583714No Hit
ATGGCGGGGGTTTAGGCCCAAGAAGGAAGCTTTGGTGGTGGTGTCTTGACACTTGGGT1040.11558252481134487No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA1040.11558252481134487No Hit
ATGGCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGC990.11002567265695329No Hit
ATGGCGGGGGCTCTTCGGACTTGGGCTTGGGGTCCGGCGACATGGCTAAACGCACCAA990.11002567265695329No Hit
ATGGCGGGGATTTAGTGTTTTCTTGGTCACAATTTTCTTTGCTGTCTAGCATGATCTG930.10335745007168338No Hit
ATGGCGGGGCACAAGGTAGGAGGAGTGAGCCTCATCTGGTGGGTCATCCTCAGATCTT900.10002333877904845No Hit
ATGGCGGGGCAACAAAAACAAATAATACAGATCCATGGATTAGGCTAAAGTATATATT900.10002333877904845No Hit
ATGGCGGGGATAAACAGTAGATGTTTGAGATTGAGAGATGGGAATTACTGTCATCTAA900.10002333877904845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCGT150.00709300652.1945238
GGACTAA550.052.1945238
GGATCGG150.00709300652.1945238
GACCCTA150.00709300652.1945239
GATTGGA150.00709300652.1945239
GATCTAC150.00709300652.1945239
GGACAGG150.00709300652.1945239
GATTCGT150.00709300652.1945239
GACCTTA150.00709300652.1945239
AACACGG1100.052.19452310
GGACTCC251.6069487E-552.194528
GACTTAT251.6069487E-552.194529
CGGGGAT13500.052.194525
GGTGGCA251.6069487E-552.194528
TAGTGAA203.4052136E-452.07808339
CGGTGTC353.801324E-852.04905741
TATCACC150.007171781752.04905344
GGTAAGG150.007171781752.04905344
TAGAAGC150.007171781752.04905338
CAATTTG150.007171781752.04905338
Produced by FastQC (version 0.11.5)