SRR212723_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212723_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84894
Sequences flagged as poor quality	0
Sequence length	56
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1186	1.3970363040968738	No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	914	1.0766367470021438	No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.44643908874596555	No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	346	0.4075670836572667	No Hit
ATGGCGGGGAGCCCAGCTGTCTTGTCTCCCTCTGGCTCTGCTGGGATCCTAGGCCC	313	0.36869507856856787	No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	263	0.3097981011614484	No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGGGTCCATCCTGAC	231	0.2721040356208919	No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	207	0.24383348646547456	No Hit
ATGGCGGGGTGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT	205	0.2414776073691898	No Hit
ATGGCGGGGTGGCATCCTTTCTACGGTATCCTTGCTTTGACAACTGCAGTAGACTC	174	0.20496148137677575	No Hit
ATGGCGGGGATTTGATTTTGCTTTTGCGTGATAACTTTACTTCCCAATGACTTCTT	165	0.19436002544349426	No Hit
ATGGCGGGGATTTCTATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCAC	163	0.19200414634720947	No Hit
ATGGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	161	0.18964826725092468	No Hit
ATGGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132	0.1554880203547954	No Hit
ATGGCGGGGATCCTGATGACTACCACACATACTGGCTATTTATATATAAATATATG	128	0.15077626216222584	No Hit
ATGGCGGGGGGTAAGCAGTCATTGCTCCTGACTTTGGTCACTCAGCAAACCGTTTG	126	0.14842038306594105	No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	119	0.14017480622894432	No Hit
ATGGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	113	0.13310716894009	No Hit
ATGGCGGGGTACAGGACTTGGCTTATCTGTATCACTGCCTTTTTATACAAGCCAGA	108	0.12721747119937804	No Hit
ATGGCGGGGGTTTAGGCCCAAGAAGGAAGCTTTGGTGGTGGTGTCTTGACACTTGG	101	0.11897189436238131	No Hit
ATGGCGGGGATAAACAGTAGATGTTTGAGATTGAGAGATGGGAATTACTGTCATCT	99	0.11661601526609655	No Hit
ATGGCGGGGGGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT	98	0.11543807571795416	No Hit
ATGGCGGGGCTGTAAACGTTATCTTTTTAAATTGAATGTGCACAAATAAAAGTTTG	96	0.11308219662166938	No Hit
ATGGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91	0.10719249888095743	No Hit
ATGGCGGGGATTTAGTGTTTTCTTGGTCACAATTTTCTTTGCTGTCTAGCATGATC	91	0.10719249888095743	No Hit
ATGGCGGGGAAGTATTACAAATGCTGATGTGCTATGGTTAGTGAATTGTCAAGAAT	90	0.10601455933281505	No Hit
ATGGCGGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT	90	0.10601455933281505	No Hit
ATGGCGGGGCACAAGGTAGGAGGAGTGAGCCTCATCTGGTGGGTCATCCTCAGATC	87	0.10248074068838788	No Hit
ATGGCGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85	0.1001248615921031	No Hit
ATGGCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG	85	0.1001248615921031	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTCAC	20	4.049083E-4	50.27867	50
GACGCAT	25	2.003894E-5	50.27867	48
CGCATAG	30	9.972682E-7	50.278667	50
GACATCC	15	0.008221447	50.278667	49
AACCGGC	15	0.008279573	50.189205	46
ACCGGCT	15	0.008279573	50.189205	47
GCAGTAG	45	1.2914825E-10	50.189205	46
CATCCTC	15	0.008279573	50.189205	45
CCCAATG	25	2.0322723E-5	50.15946	43
CTAACAG	20	4.0968432E-4	50.15946	43
TAACAGT	20	4.0968432E-4	50.15946	44
CGAGAGA	20	4.0968432E-4	50.15946	42
GTCATCC	15	0.008299016	50.159454	43
TATCACC	15	0.008299016	50.159454	44
TAAACCG	15	0.008299016	50.159454	44
AACTGCA	45	1.2914825E-10	50.159454	42
GCGAGAG	20	4.108853E-4	50.129738	41
CTGGTGG	20	4.1208905E-4	50.100063	36
TTATCAC	25	2.0465859E-5	50.10006	38
CACCGAC	35	5.1300958E-8	50.10006	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content