##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212723_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 84894 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.032475793342286 38.0 35.0 38.0 35.0 39.0 2 36.94830023323203 38.0 35.0 38.0 35.0 39.0 3 36.77828821824864 38.0 35.0 38.0 35.0 39.0 4 36.73488114589959 38.0 35.0 38.0 35.0 39.0 5 36.26878224609513 38.0 35.0 38.0 33.0 39.0 6 36.74262020873089 38.0 35.0 38.0 35.0 39.0 7 36.90664829080971 38.0 35.0 38.0 35.0 39.0 8 35.16678446062148 35.0 35.0 36.0 35.0 39.0 9 35.13673522274837 35.0 35.0 36.0 35.0 39.0 10 29.402902443046624 32.0 24.0 35.0 16.0 37.0 11 30.002768157938135 32.0 27.0 35.0 18.0 38.0 12 31.256508116003488 33.0 29.0 36.0 22.0 38.0 13 31.640375055952127 34.0 29.0 36.0 22.0 38.0 14 31.67339270148656 33.0 29.0 36.0 23.0 38.0 15 31.55003887200509 33.0 29.0 36.0 23.0 38.0 16 30.396459113718283 33.0 28.0 36.0 20.0 38.0 17 29.797618206233658 33.0 27.0 36.0 19.0 38.0 18 29.689353782363888 32.0 27.0 35.0 19.0 38.0 19 29.46102198035197 32.0 27.0 35.0 18.0 38.0 20 29.347845548566447 32.0 27.0 35.0 17.0 38.0 21 28.911195137465544 32.0 26.0 36.0 15.0 38.0 22 28.572207694301127 32.0 26.0 35.0 14.0 38.0 23 28.364713642895847 32.0 25.0 35.0 11.0 38.0 24 27.987148679529767 31.0 25.0 35.0 9.0 38.0 25 27.670777675689685 31.0 25.0 35.0 2.0 38.0 26 26.373936909557802 30.0 21.0 35.0 2.0 38.0 27 26.037046198789078 30.0 21.0 35.0 2.0 38.0 28 25.76324593021886 30.0 20.0 35.0 2.0 38.0 29 25.527658020590383 30.0 20.0 35.0 2.0 38.0 30 25.02242796899663 30.0 18.0 35.0 2.0 38.0 31 24.464638254764765 29.0 16.0 35.0 2.0 38.0 32 24.035385304026196 29.0 15.0 35.0 2.0 38.0 33 23.71955615237826 28.0 14.0 35.0 2.0 38.0 34 23.95077390628313 29.0 16.0 35.0 2.0 38.0 35 23.690661295262327 29.0 15.0 35.0 2.0 38.0 36 24.81435672721276 30.0 14.0 35.0 2.0 38.0 37 24.52911866563008 30.0 13.0 35.0 2.0 38.0 38 24.19688081607652 30.0 11.0 35.0 2.0 38.0 39 24.010200956486912 30.0 8.0 35.0 2.0 38.0 40 23.58819233396942 30.0 2.0 35.0 2.0 38.0 41 23.0306264282517 30.0 2.0 35.0 2.0 38.0 42 22.721523311423656 29.0 2.0 35.0 2.0 38.0 43 22.503416024689614 29.0 2.0 35.0 2.0 38.0 44 22.023040497561666 29.0 2.0 35.0 2.0 38.0 45 21.558190213678234 29.0 2.0 35.0 2.0 38.0 46 20.747862039720122 28.0 2.0 35.0 2.0 38.0 47 20.41345678139798 27.0 2.0 35.0 2.0 38.0 48 20.057471670553866 26.0 2.0 35.0 2.0 38.0 49 19.79010295191651 26.0 2.0 35.0 2.0 37.0 50 19.284024783848093 25.0 2.0 35.0 2.0 37.0 51 18.680931514594672 24.0 2.0 35.0 2.0 37.0 52 18.36473720168681 23.0 2.0 35.0 2.0 37.0 53 18.014182392159633 22.0 2.0 34.0 2.0 37.0 54 17.55466817442929 18.0 2.0 34.0 2.0 36.0 55 17.063231794944283 2.0 2.0 34.0 2.0 36.0 56 9.8014582891606 2.0 2.0 19.0 2.0 24.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.45803215353409144 1 2 -0.29216704977682895 1 3 -0.5137478209564534 1 4 -0.22605159677823394 1 5 -0.6815731101887437 1 6 -1.0007001486545946 1 7 -1.5499863441596418 1 8 -1.4859451297182247 1 9 -1.7414521191047783 1 10 -1.7229594440386187 1 11 -0.36356197814258095 1 12 -1.8692632174186663 1 13 -2.0547768748667146 1 14 -0.8975726434550211 1 15 -1.8412444515777686 1 16 -0.9946514172465726 1 17 -0.19011596745416526 1 18 -1.083580036870611 1 19 -0.6190909548856389 1 20 0.005006533847748074 1 21 -0.6783140734960789 1 22 0.12973464043739114 1 23 -1.9648369849990601 1 24 -0.7702909350601743 1 25 -0.7203652137919789 1 26 -0.6699880844246593 1 27 -0.5408149454351694 1 28 -0.19289510621286965 1 29 0.23559228003195543 1 30 0.4888239235474323 1 31 1.846695155512318 1 32 1.6709531536555495 1 33 3.230553087297668 1 34 2.667920746875751 1 35 2.2655246276661245 1 36 1.0597388721240577 1 37 1.5240880583013343 1 38 2.1106401972151616 1 39 1.9047278839478352 1 40 2.198124753237508 1 41 1.8097470099413648 1 42 2.3396213082458104 1 43 3.0309071104087835 1 44 2.498871492297649 1 45 2.6765190319497947 1 46 2.741022526708832 1 47 2.640528302857131 1 48 2.716964952152491 1 49 2.256332593061771 1 50 1.0903808452079282 1 51 1.2059533286004083 1 52 2.0370236014011702 1 53 1.473985894247651 1 54 1.2665239808916908 1 55 2.155602397604504 1 56 0.9040011407029347 2 1 0.07227087676893973 2 2 0.05252991992013989 2 3 -0.6677882249968548 2 4 0.4204130496864096 2 5 -0.4138963425119755 2 6 0.07126954831510801 2 7 0.19243789826460045 2 8 -0.5490764428495396 2 9 -0.4043309069835672 2 10 0.07754560646643327 2 11 -1.7991680387486433 2 12 -1.2190106921661403 2 13 -1.4424031374929775 2 14 -0.08696658284895875 2 15 -0.9346787950121112 2 16 -1.4908635384586937 2 17 -1.1370856644238607 2 18 -1.670701248991822 2 19 -0.6557071165018016 2 20 -1.347266193424982 2 21 -1.070990841172847 2 22 -1.8437502080474566 2 23 -1.697160217322292 2 24 -0.45463436940361035 2 25 -0.23172885015561562 2 26 -0.1510486904852648 2 27 -0.2327341373543632 2 28 -0.4769860153037797 2 29 -0.8288016593619822 2 30 -0.5389538542303427 2 31 0.5588163676335292 2 32 1.9247410324434284 2 33 1.4767652085097893 2 34 1.1451934741484813 2 35 0.46754482968632516 2 36 1.7870115993967843 2 37 1.2753506845639606 2 38 1.6447311063060717 2 39 1.7633137425336933 2 40 1.4216096017223592 2 41 1.733989434183787 2 42 1.5353283789528795 2 43 2.4096949891966624 2 44 2.822103815529971 2 45 1.4265190319497947 2 46 2.0869821226684273 2 47 1.76931618164501 2 48 1.9088841440716848 2 49 2.55431239104157 2 50 3.007047511874596 2 51 1.1440846417317232 2 52 1.088791278168845 2 53 1.0055515508133084 2 54 0.921827011194722 2 55 1.2225215895236978 2 56 0.8939001306019261 3 1 0.11174456097946717 3 2 0.33542465676224253 3 3 0.31759189196220916 3 4 0.452576792376469 3 5 0.1489691545640497 3 6 0.19188358340282718 3 7 0.38980631931723053 3 8 0.42314577937268183 3 9 0.03645856670064518 3 10 -1.0160216449955541 3 11 -0.14785224927495833 3 12 -0.8820223880725706 3 13 -0.25015167550467154 3 14 0.01829657504577753 3 15 -1.541403941210941 3 16 -0.5632319595113238 3 17 -0.40901548898526485 3 18 -0.1992100209216474 3 19 0.14326949168533432 3 20 1.1812425785048433 3 21 -0.26324230316115305 3 22 -1.9775221378720182 3 23 -1.4500841939304792 3 24 -1.1446928489357724 3 25 -2.2207639378749136 3 26 -2.49973290101158 3 27 -2.1727926168865253 3 28 -1.733564962672201 3 29 -1.1599420102391775 3 30 -1.976088357154321 3 31 -0.45872749201559415 3 32 -0.9678028272056949 3 33 -1.139462861665649 3 34 -0.7846310872550291 3 35 0.19415301682083097 3 36 -1.002462084813743 3 37 -0.8730411283015336 3 38 -1.4100934550974387 3 39 -0.14311900600431926 3 40 0.43257451400306124 3 41 0.5190771534820335 3 42 0.41179036725697316 3 43 0.5412739365650836 3 44 0.403975160559213 3 45 -0.03839324875195871 3 46 0.6206078536625803 3 47 0.6114214448029038 3 48 0.9849075358845489 3 49 0.8233182389947871 3 50 0.07722295047108574 3 51 -0.5145410892624298 3 52 0.17577958226241464 3 53 0.18172114145658114 3 54 1.0336691164578795 3 55 1.449860770810247 3 56 0.9977013001925688 4 1 0.10930791380597782 4 2 0.4120978211547097 4 3 0.37850807129944286 4 4 0.5932525558592516 4 5 0.6972147685991317 4 6 0.49873868411757627 4 7 0.6986107377707711 4 8 0.861417384310954 4 9 1.0570888461028503 4 10 0.4016196805405059 4 11 1.2980541834735781 4 12 1.2424090609202771 4 13 0.5591400723835669 4 14 2.1938976146819087 4 15 0.8214940444940595 4 16 0.7359883133931575 4 17 0.08822297755144604 4 18 0.4759036892797823 4 19 1.3674410316463472 4 20 1.5863757818836604 4 21 0.6836387884567827 4 22 0.29359547096488825 4 23 0.22876570860363188 4 24 0.27067427257169996 4 25 0.9827773226838907 4 26 0.4029636551937479 4 27 1.1438090725221812 4 28 0.2128287995110334 4 29 -0.6420732643002545 4 30 -0.07907731102046611 4 31 0.8489398244236526 4 32 1.6731978225668875 4 33 1.135715825793742 4 34 0.8828477951361329 4 35 1.4737176691924994 4 36 1.2746659203844395 4 37 1.2244247586380368 4 38 1.0135582667998975 4 39 2.07812855734851 4 40 1.7996960214754445 4 41 0.4809030144307016 4 42 -0.18226421363971212 4 43 0.12574437191271315 4 44 -0.27048877706262076 4 45 0.2567659455300415 4 46 0.8555006411869464 4 47 0.5440075396696997 4 48 0.6311063662939063 4 49 0.26418893425144674 4 50 0.08420800570175402 4 51 -0.3389400496263022 4 52 0.3063838707614366 4 53 0.09968735328244449 4 54 0.4449751593428708 4 55 1.1870277623632042 4 56 -0.04437147433634614 5 1 -0.4639610072890292 5 2 -0.2427599351523213 5 3 -0.4491891911804302 5 4 -0.04818598413001496 5 5 -0.9984374053139078 5 6 -0.984889871974751 5 7 -0.4162577539093135 5 8 -0.19279624961282593 5 9 -0.2757077185777703 5 10 0.3323765243408303 5 11 -0.8082260097631355 5 12 0.585940998655115 5 13 0.6197949301398715 5 14 0.37317834468727185 5 15 -0.8247754133696006 5 16 1.0435567513963804 5 17 0.4897259297790377 5 18 0.9434291857907837 5 19 0.48499336658998615 5 20 0.42266134280690437 5 21 1.2472458738029992 5 22 0.4986410963003678 5 23 1.848733502484471 5 24 1.6770081426736851 5 25 2.3316769469458336 5 26 2.115255657340821 5 27 2.793835910954815 5 28 2.658883549913611 5 29 1.4990968913626546 5 30 1.3728818945619246 5 31 1.638526512561068 5 32 2.4627845107042994 5 33 1.6923449186547188 5 34 1.6633094161774657 5 35 0.9868685011839098 5 36 0.7942579762083781 5 37 1.58996421113401 5 38 0.27335429471186856 5 39 0.6208016652390071 5 40 0.4958849640411991 5 41 0.3354387095461071 5 42 1.5939032581799317 5 43 1.061868902240139 5 44 0.16751444354929745 5 45 0.6620262783266071 5 46 1.1244217361949964 5 47 1.2874321236739945 5 48 1.679415545037866 5 49 1.498756835486013 5 50 1.9382069321644515 5 51 2.9997609702341386 5 52 2.506061809569811 5 53 2.432483917963065 5 54 0.9779864314845774 5 55 2.371676178895676 5 56 0.04365858470820605 6 1 0.07227087676893973 6 2 0.19018814776823945 6 3 0.31709222507346624 6 4 0.3036760595317034 6 5 0.9039658234514576 6 6 0.23635393650076253 6 7 -0.22528362072274177 6 8 -0.026221309234912837 6 9 0.021302004408838116 6 10 -0.2603587535898271 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0.6687842458200421 6 53 1.1692294692380614 6 54 1.0942953656251007 6 55 0.7033584390314331 6 56 1.318653998393767 7 1 0.4538498241373574 7 2 0.6775299199201399 7 3 0.43601294459378437 7 4 0.452576792376469 7 5 0.8068638914061523 7 6 0.4418835834028272 7 7 0.42928000352775797 7 8 0.5810405162147845 7 9 0.615405935121693 7 10 1.0760836181623397 7 11 0.7600424875671479 7 12 0.6969249803484807 7 13 0.7893220087058559 7 14 0.8998755224141988 7 15 0.7743855324732678 7 16 0.9762417246992001 7 17 1.0251950373305228 7 18 0.09026366328888002 7 19 1.7485326495800706 7 20 1.2207162627153707 7 21 0.8814945389441107 7 22 1.3382673358121906 7 23 3.181494753437942 7 24 2.61846504580107 7 25 2.4502886937040316 7 26 3.618688151619999 7 27 4.392996856797684 7 28 2.884856089959378 7 29 3.037426410813456 7 30 3.20812216916147 7 31 2.9886409290370395 7 32 3.9006182254258874 7 33 2.571063454123827 7 34 3.768000491692341 7 35 3.944153016820831 7 36 4.708064230975733 7 37 3.929590450645833 7 38 4.392538123849931 7 39 4.462144151890421 7 40 4.972048198213585 7 41 3.479603469271506 7 42 2.8723166830464493 7 43 3.4886423576177137 7 44 4.153975160559213 7 45 3.6458172775638324 7 46 3.804818379978368 7 47 4.5587898658555375 7 48 4.090170693779285 7 49 4.994370870573732 7 50 5.064065055734243 7 51 5.301248384421783 7 52 4.873148003315045 7 53 5.958036930930266 7 54 6.125774379615773 7 55 5.673544981336562 7 56 3.7345434054557263 8 1 0.16686547136353624 8 2 0.015367757757978495 8 3 0.007136699928068424 8 4 -0.04955692028355685 8 5 -0.6045870332026695 8 6 0.13320648525204604 8 7 0.28703249285919696 8 8 0.09882145504835904 8 9 0.11931774166507836 8 10 0.847065125985953 8 11 -2.1944383090189135 8 12 -0.8500166981721442 8 13 -0.5531388732287112 8 14 -0.9450746909570675 8 15 -0.7867808971142125 8 16 -0.9537013762965323 8 17 -0.9456442229824198 8 18 -0.7326381859287601 8 19 -1.414340750135434 8 20 -0.8123562835150722 8 21 -1.8183632135452186 8 22 -0.3989303882276367 8 23 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0.10269430852100925 116 8 0.16673552296242633 116 9 0.2911819135292504 116 10 0.934383213304038 116 11 1.8835242689436633 116 12 0.9337670856116382 116 13 1.3706310505412098 116 14 1.1758539299715522 116 15 1.1529280425947235 116 16 1.5379826153874596 116 17 2.061632284294088 116 18 2.26840131511074 116 19 2.351771515976832 116 20 1.867477396318609 116 21 0.49418009899809334 116 22 0.2812497919525434 116 23 0.6318996117375342 116 24 1.46630580153656 116 25 1.8099378165110487 116 26 0.621387206950633 116 27 1.1305137258935005 116 28 1.8723729590551947 116 29 1.6038906483303244 116 30 1.3477982825217936 116 31 2.3536881625053248 116 32 1.5241000068024029 116 33 1.0697139264585083 116 34 1.6772447561997623 116 35 2.869254231395729 116 36 2.0177808301660143 116 37 1.8725729067861856 116 38 1.7889618755368382 116 39 2.0282710074909573 116 40 2.495327550440308 116 41 2.1570663572607103 116 42 3.0086189772434793 116 43 2.6148231943248668 116 44 2.1340696274957835 116 45 2.4457498011805647 116 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30 2.737769071226758 119 31 1.7381412621482966 119 32 2.0771671927809834 119 33 0.6323559258093674 119 34 1.6589065543172552 119 35 0.9281636763811214 119 36 2.4262521057258972 119 37 1.9590707970815444 119 38 0.6378745662216829 119 39 1.192990254488688 119 40 2.2913353401189838 119 41 0.8120485059137472 119 42 1.479245397236987 119 43 1.5615937233738784 119 44 0.5612036748829965 119 45 1.5457173441860839 119 46 1.757516581177569 119 47 3.02564529556906 119 48 2.714088082187015 119 49 2.1274488186083786 119 50 1.8435454021699549 119 51 2.9529805629694152 119 52 2.542201967339029 119 53 2.485685165440593 119 54 2.524675112460546 119 55 1.3236116035883931 119 56 0.8629577958621226 120 1 -1.9883351838371226 120 2 -1.9891367467465244 120 3 -1.528899336107969 120 4 -2.362415233141867 120 5 -1.5603609889766261 120 6 -2.121912269866712 120 7 -3.0954408896141885 120 8 -2.728369372142467 120 9 -2.680846058498716 120 10 -2.1472018682810408 120 11 -0.06053167511227997 120 12 -1.0662329143883618 120 13 -0.1456859657758045 120 14 -1.2309059767883532 120 15 -0.7503353606686787 120 16 1.3538334312382752 120 17 1.3553385780003815 120 18 -0.17448912777970094 120 19 1.1384848026901189 120 20 1.974703503544717 120 21 1.5489586537766478 120 22 2.6600376707404223 120 23 1.3381933180312409 120 24 1.6691030043337634 120 25 1.582665089238322 120 26 1.9209210064844306 120 27 1.6864577818375572 120 28 2.155589742271978 120 29 2.5537740982137755 120 30 2.867611802335311 120 31 4.40730121611838 120 32 3.807316790019186 120 33 3.7002500569946406 120 34 4.501254080209087 120 35 4.37158523372673 120 36 4.908223720608905 120 37 3.614997149210428 120 38 4.837912924487888 120 39 4.344121823341776 120 40 4.289033844146601 120 41 4.461262161456517 120 42 4.203257671882174 120 43 2.7430283225299945 120 44 3.923113916540075 120 45 4.918943274374037 120 46 5.528901314587621 120 47 5.034467696796526 120 48 6.974540709728249 120 49 6.6048174415466185 120 50 6.317653572480655 120 51 6.827165449812529 120 52 7.62793269231026 120 53 6.867925288187045 120 54 8.115008829376539 120 55 7.625299367301473 120 56 4.0706678073696025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108.0 3 83.0 4 92.0 5 145.0 6 107.0 7 742.0 8 276.0 9 831.0 10 1358.0 11 1018.0 12 1535.0 13 1789.0 14 1868.0 15 2088.0 16 2042.0 17 1801.0 18 1866.0 19 1680.0 20 1737.0 21 2019.0 22 2288.0 23 2530.0 24 2719.0 25 2895.0 26 3332.0 27 3718.0 28 4021.0 29 4633.0 30 5060.0 31 5349.0 32 5317.0 33 5332.0 34 4763.0 35 3118.0 36 1766.0 37 1991.0 38 1850.0 39 1027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 41.418710391782696 45.550922326666196 4.125144297594647 8.905222983956463 11 27.10556694230452 24.243173840318516 30.439135863547484 18.212123353829483 12 19.679836030814897 16.395740570593915 35.148538177020754 28.77588522157043 13 16.197846726505997 26.62025584846986 35.56788465615945 21.6140127688647 14 20.398379155181757 31.98694842980658 27.554361910146767 20.06031050486489 15 22.908568332273187 31.87740005182934 25.70499681956322 19.50903479633425 16 28.13626404692911 33.94468395881923 21.63757155982755 16.281480434424108 17 21.880227106744883 34.237990906306685 23.756684806935706 20.125097180012723 18 21.81072867340448 33.052983720875446 25.684971847244796 19.451315758475275 19 21.15932810328174 34.87172238320729 26.07251395858364 17.89643555492732 20 24.392772162932598 32.38862581572314 26.13376681508705 17.084835206257214 21 22.82257874525879 31.577025467053033 25.867552477206868 19.732843310481307 22 20.659881734869366 30.148184795156315 28.848917473555257 20.343015996419066 23 19.38417320423116 31.789054585718663 28.823002803496124 20.003769406554056 24 20.694041981765494 32.51584328692251 25.856951021273588 20.933163710038404 25 20.99322533136966 37.1181148748159 23.314285714285717 18.57437407952872 26 21.978009027376757 35.97044300142599 22.870140124686223 19.181407846511025 27 20.4526967285588 32.689655172413794 25.027998821102273 21.82964927792514 28 21.567795111252607 32.39473156696971 24.647729550626718 21.389743771150965 29 22.914061947111417 30.970017220636453 24.240994550730107 21.874926281522022 30 21.38390012507965 30.328275080829776 25.5233285346801 22.764496259410475 31 23.40724307612428 32.43807748132589 23.87925846382592 20.275420978723908 32 23.57427016562194 31.488236474601884 25.069943690902008 19.867549668874172 33 23.26180156133741 32.09363300303528 26.105750493085 18.538814942542313 34 23.319709891563704 33.00810319166529 25.460086465543718 18.212100451227293 35 22.27971928829659 33.59207013066799 26.130053637673967 17.99815694336145 36 22.15813733601229 34.52074222905094 24.334003072922822 18.987117362013947 37 22.349241627161927 33.35303644681933 24.74316991571008 19.55455201030867 38 20.868855754336593 34.69629069066229 25.227323787113786 19.207529767887337 39 23.774834437086092 32.760170293282876 24.66414380321665 18.800851466414382 40 23.59837685000059 33.40707702864174 23.48125451039313 19.513291610964544 41 21.966282165039928 33.774622892635314 23.770482105885833 20.488612836438925 42 21.26793238956489 32.60262298186639 24.764452440698832 21.36499218786989 43 23.835901806216413 31.218189995975663 24.418246810122387 20.527661387685534 44 23.089852396340092 32.41362167536664 23.81307481978623 20.683451108507036 45 23.531431683895185 32.60748582051555 23.116999988159094 20.744082507430168 46 22.372487950451784 34.538090783131814 23.45013796288621 19.639283303530192 47 20.12627788241705 32.98861603705415 24.987857895921437 21.897248184607363 48 21.068724001848757 33.738637845012505 23.239828871428404 21.952809281710337 49 21.512165075454266 34.35715808675464 23.147994598564356 20.98268223922674 50 20.817879107466613 32.78389363542642 25.52465368709192 20.87357357001505 51 20.59130228700083 32.90674250503614 25.564640360232254 20.93731484773077 52 21.53041994097358 33.076129858123245 24.247057568538207 21.146392632364968 53 21.28842574480445 33.25271781010302 26.13247027302581 19.32638617206672 54 21.065053667793393 33.113918045424896 24.65753424657534 21.16349404020637 55 20.220069483145 32.923864970297736 24.73647390824905 22.11959163830822 56 20.537566840164565 32.74723450671662 22.934920503183427 23.780278149935384 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 220.5 13 1265.0 14 2089.0 15 2570.5 16 3052.0 17 2458.5 18 1865.0 19 1517.0 20 1169.0 21 1008.0 22 688.0 23 529.0 24 546.5 25 564.0 26 602.5 27 641.0 28 711.5 29 833.5 30 885.0 31 1255.0 32 1625.0 33 1709.5 34 1794.0 35 1946.5 36 2099.0 37 2362.0 38 2692.0 39 2759.0 40 3079.5 41 3400.0 42 3294.5 43 3189.0 44 3279.0 45 3369.0 46 3523.5 47 3641.0 48 3604.0 49 3452.5 50 3301.0 51 3518.5 52 3736.0 53 3875.0 54 3944.0 55 3874.0 56 3700.0 57 3526.0 58 3505.5 59 3485.0 60 3436.0 61 3387.0 62 3412.5 63 3477.5 64 3517.0 65 3341.0 66 3165.0 67 2732.0 68 2299.0 69 2038.0 70 1777.0 71 1497.0 72 1100.0 73 983.0 74 857.0 75 731.0 76 617.0 77 503.0 78 428.5 79 283.0 80 212.0 81 184.0 82 156.0 83 127.0 84 98.0 85 73.0 86 48.0 87 33.5 88 19.0 89 19.0 90 12.0 91 5.0 92 5.5 93 6.0 94 6.0 95 6.0 96 9.0 97 6.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.022380851414705395 26 0.04829552147383796 27 0.08127782882182487 28 0.10248074068838788 29 0.13192922939194762 30 0.1743350531250736 31 0.17786887176950078 32 0.21556293731005727 33 0.2626805192357528 34 0.2779937333616039 35 0.2968407661318821 36 0.3345348316724386 37 0.35927156218342876 38 0.38047447404999174 39 0.39343180907955805 40 0.43230381416825686 41 0.4346596932645417 42 0.4829552147383796 43 0.4805993356420949 44 0.484133154286522 45 0.5194713407307937 46 0.5312507362122176 47 0.5618771644639197 48 0.6042829881970457 49 0.5559874667232078 50 0.5948594718119066 51 0.5936815322637642 52 0.6184182627747544 53 0.6396211746413174 54 0.6820269983744435 55 0.6561123283153109 56 0.6490446910264566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 84894.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.063467382853915 #Duplication Level Percentage of deduplicated Percentage of total 1 73.96760042153763 35.55139350248545 2 10.739406416194889 10.3234621999199 3 4.992279979413278 7.1983885786981405 4 2.9679190255618457 5.705939171201734 5 1.926328946401 4.62930242419959 6 1.3430385020709261 3.873065234292176 7 0.9337548709653701 3.1415647748957527 8 0.7033796534568536 2.704549202534926 9 0.409283631105556 1.770443140858011 >10 1.872411342303262 15.251961269347659 >50 0.09558120726417176 3.3312130421466772 >100 0.044114403352694655 4.045044408320965 >500 0.002450800186260814 1.0766367470021438 >1k 0.002450800186260814 1.3970363040968738 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1186 1.3970363040968738 No Hit ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 914 1.0766367470021438 No Hit ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 379 0.44643908874596555 No Hit ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 346 0.4075670836572667 No Hit ATGGCGGGGAGCCCAGCTGTCTTGTCTCCCTCTGGCTCTGCTGGGATCCTAGGCCC 313 0.36869507856856787 No Hit ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 263 0.3097981011614484 No Hit ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGGGTCCATCCTGAC 231 0.2721040356208919 No Hit ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 207 0.24383348646547456 No Hit ATGGCGGGGTGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT 205 0.2414776073691898 No Hit ATGGCGGGGTGGCATCCTTTCTACGGTATCCTTGCTTTGACAACTGCAGTAGACTC 174 0.20496148137677575 No Hit ATGGCGGGGATTTGATTTTGCTTTTGCGTGATAACTTTACTTCCCAATGACTTCTT 165 0.19436002544349426 No Hit ATGGCGGGGATTTCTATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCAC 163 0.19200414634720947 No Hit ATGGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 161 0.18964826725092468 No Hit ATGGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 132 0.1554880203547954 No Hit ATGGCGGGGATCCTGATGACTACCACACATACTGGCTATTTATATATAAATATATG 128 0.15077626216222584 No Hit ATGGCGGGGGGTAAGCAGTCATTGCTCCTGACTTTGGTCACTCAGCAAACCGTTTG 126 0.14842038306594105 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 119 0.14017480622894432 No Hit ATGGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 113 0.13310716894009 No Hit ATGGCGGGGTACAGGACTTGGCTTATCTGTATCACTGCCTTTTTATACAAGCCAGA 108 0.12721747119937804 No Hit ATGGCGGGGGTTTAGGCCCAAGAAGGAAGCTTTGGTGGTGGTGTCTTGACACTTGG 101 0.11897189436238131 No Hit ATGGCGGGGATAAACAGTAGATGTTTGAGATTGAGAGATGGGAATTACTGTCATCT 99 0.11661601526609655 No Hit ATGGCGGGGGGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT 98 0.11543807571795416 No Hit ATGGCGGGGCTGTAAACGTTATCTTTTTAAATTGAATGTGCACAAATAAAAGTTTG 96 0.11308219662166938 No Hit ATGGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.10719249888095743 No Hit ATGGCGGGGATTTAGTGTTTTCTTGGTCACAATTTTCTTTGCTGTCTAGCATGATC 91 0.10719249888095743 No Hit ATGGCGGGGAAGTATTACAAATGCTGATGTGCTATGGTTAGTGAATTGTCAAGAAT 90 0.10601455933281505 No Hit ATGGCGGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT 90 0.10601455933281505 No Hit ATGGCGGGGCACAAGGTAGGAGGAGTGAGCCTCATCTGGTGGGTCATCCTCAGATC 87 0.10248074068838788 No Hit ATGGCGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85 0.1001248615921031 No Hit ATGGCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG 85 0.1001248615921031 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0011779395481423894 0.0 0.0 0.0 0.0 11 0.0011779395481423894 0.0 0.0 0.0 0.0 12 0.0011779395481423894 0.0 0.0 0.0 0.0 13 0.0011779395481423894 0.0 0.0 0.0 0.0 14 0.0023558790962847787 0.0 0.0 0.0 0.0 15 0.0023558790962847787 0.0 0.0 0.0 0.0 16 0.0023558790962847787 0.0 0.0 0.0 0.0 17 0.003533818644427168 0.0 0.0 0.0 0.0 18 0.0047117581925695574 0.0 0.0 0.0 0.0 19 0.0047117581925695574 0.0 0.0 0.0 0.0 20 0.0047117581925695574 0.0 0.0 0.0 0.0 21 0.0047117581925695574 0.0 0.0 0.0 0.0 22 0.0047117581925695574 0.0 0.0 0.0 0.0 23 0.008245576836996725 0.0 0.0 0.0 0.0 24 0.008245576836996725 0.0 0.0 0.0 0.0 25 0.008245576836996725 0.0 0.0 0.0 0.0 26 0.011779395481423894 0.0 0.0 0.0 0.0 27 0.011779395481423894 0.0 0.0 0.0 0.0 28 0.012957335029566282 0.0 0.0 0.0 0.0 29 0.012957335029566282 0.0 0.0 0.0 0.0 30 0.012957335029566282 0.0 0.0 0.0 0.0 31 0.012957335029566282 0.0 0.0 0.0 0.0 32 0.012957335029566282 0.0 0.0 0.0 0.0 33 0.014135274577708672 0.0 0.0 0.0 0.0 34 0.014135274577708672 0.0 0.0 0.0 0.0 35 0.014135274577708672 0.0 0.0 0.0 0.0 36 0.014135274577708672 0.0 0.0 0.0 0.0 37 0.01531321412585106 0.0 0.0 0.0 0.0 38 0.01531321412585106 0.0 0.0 0.0 0.0 39 0.01649115367399345 0.0 0.0 0.0 0.0 40 0.01649115367399345 0.0 0.0 0.0 0.0 41 0.01649115367399345 0.0 0.0 0.0 0.0 42 0.01649115367399345 0.0 0.0 0.0 0.0 43 0.01649115367399345 0.0 0.0 0.0 0.0 44 0.01649115367399345 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTCAC 20 4.049083E-4 50.27867 50 GACGCAT 25 2.003894E-5 50.27867 48 CGCATAG 30 9.972682E-7 50.278667 50 GACATCC 15 0.008221447 50.278667 49 AACCGGC 15 0.008279573 50.189205 46 ACCGGCT 15 0.008279573 50.189205 47 GCAGTAG 45 1.2914825E-10 50.189205 46 CATCCTC 15 0.008279573 50.189205 45 CCCAATG 25 2.0322723E-5 50.15946 43 CTAACAG 20 4.0968432E-4 50.15946 43 TAACAGT 20 4.0968432E-4 50.15946 44 CGAGAGA 20 4.0968432E-4 50.15946 42 GTCATCC 15 0.008299016 50.159454 43 TATCACC 15 0.008299016 50.159454 44 TAAACCG 15 0.008299016 50.159454 44 AACTGCA 45 1.2914825E-10 50.159454 42 GCGAGAG 20 4.108853E-4 50.129738 41 CTGGTGG 20 4.1208905E-4 50.100063 36 TTATCAC 25 2.0465859E-5 50.10006 38 CACCGAC 35 5.1300958E-8 50.10006 40 >>END_MODULE