FastQCFastQC Report
Tue 31 May 2016
SRR212722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82007
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9781.1925811211238064No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8100.9877205604399624No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3980.485324423524821No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.4036240808711451No Hit
ATGGCGGGGAGCCCAGCTGTCTTGTCTCCCTCTGGCTCTGCTGGGATCCTAGGCCC3130.3816747350835904No Hit
ATGGCGGGGTGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT2450.2987549843306059No Hit
ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGGGTCCATCCTGAC2220.2707085980465082No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.24875925225895348No Hit
ATGGCGGGGTGGCATCCTTTCTACGGTATCCTTGCTTTGACAACTGCAGTAGACTC1950.23778457936517614No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.20364115258453547No Hit
ATGGCGGGGGGTAAGCAGTCATTGCTCCTGACTTTGGTCACTCAGCAAACCGTTTG1610.1963247039886839No Hit
ATGGCGGGGATTTGATTTTGCTTTTGCGTGATAACTTTACTTCCCAATGACTTCTT1450.17681417439974637No Hit
ATGGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.1707171339032034No Hit
ATGGCGGGGATTTCTATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCAC1360.16583950150596902No Hit
ATGGCGGGGAAGTATTACAAATGCTGATGTGCTATGGTTAGTGAATTGTCAAGAAT1300.15852305291011742No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1260.15364542051288305No Hit
ATGGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.1451095638177229No Hit
ATGGCGGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT1120.1365737071225627No Hit
ATGGCGGGGATCCTGATGACTACCACACATACTGGCTATTTATATATAAATATATG1010.12316021803016816No Hit
ATGGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1010.12316021803016816No Hit
ATGGCGGGGGATTTATTTATTTATTTTATATGTATGATGCACCAGAAGAGGGCATC980.11950199373224238No Hit
ATGGCGGGGTACAGGACTTGGCTTATCTGTATCACTGCCTTTTTATACAAGCCAGA910.11096613703708219No Hit
ATGGCGGGGGGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT910.11096613703708219No Hit
ATGGCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG890.10852732083846502No Hit
ATGGCGGGGCTGTAAACGTTATCTTTTTAAATTGAATGTGCACAAATAAAAGTTTG860.10486909654053922No Hit
ATGGCGGGGAGTCATTTAACATGCATGAAATTATACCATATGTGGTCTTGTTTGAA860.10486909654053922No Hit
ATGGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.10121087224261344No Hit
ATGGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.10121087224261344No Hit
ATGGCGGGGCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCTCCCC830.10121087224261344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG204.0654864E-450.2355850
GAATAGT150.00826846150.20478449
CTAGGCC301.0070453E-650.20478449
GTAGACT402.5374902E-950.2047849
ACCGTTT451.2732926E-1050.2047849
GTCGCGT150.008308643550.14329543
CCTAGGC301.0156091E-650.14329548
GTGGAAC150.008308643550.14329543
CCCAATG252.0355701E-550.14329543
TATCTTG150.008308643550.14329542
CACAGGT150.008308643550.14329546
GACGCAT204.102583E-450.14329548
CGCGTGC150.008308643550.14329545
GCCACGA150.008308643550.14329543
CATCAGT204.102583E-450.14329542
TCGCGTG150.008308643550.14329544
AGTCGCG150.008308643550.14329542
AACCGTT451.2914825E-1050.1432948
CAACTGC451.2914825E-1050.1432941
TTATGTG150.00834897150.0819637
Produced by FastQC (version 0.11.5)