##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212722_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82007 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.17621666443109 38.0 35.0 38.0 35.0 39.0 2 37.10991744607168 38.0 35.0 38.0 35.0 39.0 3 36.9472727937859 38.0 35.0 38.0 35.0 39.0 4 36.91759240064872 38.0 35.0 38.0 35.0 39.0 5 36.55567207677393 38.0 35.0 38.0 35.0 39.0 6 36.90995890594705 38.0 35.0 38.0 35.0 39.0 7 37.03760654578268 38.0 35.0 38.0 35.0 39.0 8 35.33038643042667 35.0 35.0 36.0 35.0 39.0 9 35.30142548806809 35.0 35.0 36.0 35.0 39.0 10 29.624666187032815 32.0 24.0 35.0 18.0 37.0 11 30.43945029082883 33.0 28.0 35.0 20.0 38.0 12 31.61343543843818 33.0 29.0 36.0 23.0 38.0 13 31.96621020156816 35.0 29.0 37.0 23.0 38.0 14 32.11557549965247 34.0 30.0 36.0 23.0 38.0 15 31.901703513114732 34.0 30.0 36.0 23.0 38.0 16 30.701866913800043 33.0 28.0 36.0 21.0 38.0 17 30.17869206287268 33.0 27.0 36.0 20.0 38.0 18 30.055531844842513 33.0 27.0 36.0 19.0 38.0 19 29.759227870791516 33.0 27.0 36.0 19.0 38.0 20 29.680319972685258 33.0 27.0 36.0 18.0 38.0 21 29.063275086273123 33.0 26.0 36.0 15.0 38.0 22 28.79148121501823 32.0 26.0 35.0 14.0 38.0 23 28.570780543124368 32.0 26.0 35.0 12.0 38.0 24 28.10712500152426 32.0 25.0 35.0 8.0 38.0 25 27.826039240552635 31.0 25.0 35.0 2.0 38.0 26 26.45019327618374 30.0 22.0 35.0 2.0 38.0 27 26.06642115916934 30.0 21.0 35.0 2.0 38.0 28 25.83364834709232 30.0 21.0 35.0 2.0 38.0 29 25.546355798895217 30.0 20.0 35.0 2.0 38.0 30 25.00095113831746 30.0 18.0 35.0 2.0 38.0 31 24.3992951821186 29.0 16.0 35.0 2.0 38.0 32 23.961917885058593 29.0 15.0 35.0 2.0 38.0 33 23.663662858048703 29.0 14.0 35.0 2.0 38.0 34 23.902069335544528 29.0 15.0 35.0 2.0 38.0 35 23.58809613813455 29.0 14.0 35.0 2.0 38.0 36 24.650615191386102 30.0 12.0 35.0 2.0 38.0 37 24.299669540405088 30.0 10.0 35.0 2.0 38.0 38 24.027887863231186 30.0 8.0 35.0 2.0 38.0 39 23.844208421232334 30.0 2.0 35.0 2.0 38.0 40 23.448717792383576 30.0 2.0 35.0 2.0 38.0 41 22.837416318119185 30.0 2.0 35.0 2.0 38.0 42 22.482446620410453 29.0 2.0 35.0 2.0 38.0 43 22.243345080298024 29.0 2.0 35.0 2.0 38.0 44 21.741387930298632 29.0 2.0 35.0 2.0 38.0 45 21.287341324521076 28.0 2.0 35.0 2.0 38.0 46 20.371956052532102 27.0 2.0 35.0 2.0 38.0 47 20.039374687526674 26.0 2.0 35.0 2.0 38.0 48 19.73829063372639 26.0 2.0 35.0 2.0 37.0 49 19.507749338471108 26.0 2.0 35.0 2.0 37.0 50 18.94077334861658 24.0 2.0 34.0 2.0 37.0 51 18.339995366249223 23.0 2.0 35.0 2.0 37.0 52 17.998561098442817 22.0 2.0 34.0 2.0 37.0 53 17.631860694818734 18.0 2.0 34.0 2.0 36.0 54 17.229370663479948 12.0 2.0 34.0 2.0 36.0 55 16.69889155803773 2.0 2.0 34.0 2.0 36.0 56 9.595363810406429 2.0 2.0 19.0 2.0 24.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.8110068086081057 1 2 -0.9624055485159957 1 3 -0.9279440797097251 1 4 -0.9529198851629346 1 5 -0.9637379699670205 1 6 -1.098059770190659 1 7 -1.1361098026606555 1 8 -1.032019401572029 1 9 -1.0964601275800305 1 10 -0.6393871661144388 1 11 -0.5549437266942796 1 12 -0.9362663013081871 1 13 -1.1552417611174484 1 14 -1.6512990536524867 1 15 -1.8772298144139086 1 16 -0.7157978171779931 1 17 -0.018551053929506622 1 18 0.2452549899797134 1 19 -0.39338109349559147 1 20 -1.1003988709163686 1 21 -2.487211873888839 1 22 -2.8029048393229417 1 23 -1.9999726086329161 1 24 -2.4962677457954854 1 25 -1.673979310987395 1 26 -1.9594273725732698 1 27 -0.7829812445338113 1 28 -1.054015628085967 1 29 -1.6393360423575736 1 30 -1.5885656804360586 1 31 -0.8075675493045686 1 32 -1.1882521478827357 1 33 -1.036438884719086 1 34 -0.4852421257463604 1 35 -0.7483224002944411 1 36 0.09450399660427422 1 37 -0.14357069879716633 1 38 -0.17782933941834145 1 39 0.2770383462655097 1 40 0.2064250025825629 1 41 -0.09465871887131883 1 42 0.4136094295852786 1 43 1.0596553908610566 1 44 0.20824985084416525 1 45 0.14349753370589013 1 46 0.8351904179698977 1 47 0.8566781222977404 1 48 1.25681125368315 1 49 1.4034110354509792 1 50 1.9178432727815142 1 51 2.0783408448228293 1 52 3.229467890712513 1 53 3.313509872019143 1 54 3.0920216826279763 1 55 2.7864800159278573 1 56 1.4593977989138995 2 1 0.0802823899981675 2 2 0.011810479358572934 2 3 0.1354705544366155 2 4 -0.15291988516293742 2 5 -0.232727517005344 2 6 -0.12523747053909062 2 7 0.14925605099788442 2 8 -0.034806858018022524 2 9 0.09831339158372998 2 10 1.2686267711677885 2 11 -0.3086022632796457 2 12 -1.747416127092162 2 13 -0.3273671966575158 2 14 -0.3572223986002214 2 15 -0.8120730199888229 2 16 -0.6816514757145811 2 17 -0.9572270121176594 2 18 0.5421191014779723 2 19 0.5198593246228782 2 20 -0.11886576987107489 2 21 -0.1711839993243771 2 22 0.5514505613739225 2 23 1.0467172868374668 2 24 -0.3492294182693527 2 25 1.9260206890126064 2 26 0.23186182603300054 2 27 -1.361726889133113 2 28 -0.03694245735426094 2 29 -0.8459562514168084 2 30 0.7616085355918152 2 31 1.2938261789184295 2 32 0.6793436709325924 2 33 0.9555471779986853 2 34 1.2548275606647898 2 35 0.6116079132944101 2 36 -0.5211754459044364 2 37 0.8219345276836734 2 38 0.27861665361301746 2 39 -0.5741811659296125 2 40 -0.5650035688460093 2 41 1.0422750790380917 2 42 0.5205780707002603 2 43 1.1990282131607088 2 44 1.7626052515410287 2 45 0.5466334222076306 2 46 1.374563240269552 2 47 1.0688732442489588 2 48 -0.11461731774542017 2 49 0.8563727079248444 2 50 0.6889234121543382 2 51 0.5128356183419918 2 52 0.09183722869160604 2 53 -0.30669918721430633 2 54 -0.0696507912396207 2 55 0.4356089357884869 2 56 1.4534744539661641 3 1 -0.21582150610573336 3 2 -0.34013757258948374 3 3 -0.0943995754335134 3 4 -0.8360367682798184 3 5 -1.0677924520702788 3 6 -0.5239387692403952 3 7 -0.5052894035475717 3 8 -0.38805361126478033 3 9 -0.4718164785461383 3 10 0.5920033945444132 3 11 -0.21379706847445235 3 12 -0.4188446985207328 3 13 -0.5429516122419322 3 14 -1.070209411587232 3 15 -0.2692158771316784 3 16 -1.4647683588314635 3 17 -1.8221620770527238 3 18 -2.8046341452752728 3 19 -1.390530285766733 3 20 -1.9526320036373086 3 21 -0.7062489343893148 3 22 -0.3212767113533488 3 23 -0.21821777809759624 3 24 -1.5790995481394816 3 25 -1.2583948954029793 3 26 -1.0135927194215455 3 27 -0.23705156445778996 3 28 0.3929276725158708 3 29 -1.7771250825856377 3 30 -0.985144711161432 3 31 -1.759420574328324 3 32 -0.43753944595052374 3 33 -0.10549178304027507 3 34 -1.0490685432313143 3 35 -1.067612865926371 3 36 0.526876502147509 3 37 -0.2572862515371064 3 38 0.27861665361301746 3 39 0.15698766523921748 3 40 -0.6182503220927629 3 41 2.382534819297831 3 42 0.9270715771937681 3 43 1.6405866547191508 3 44 1.780787069722848 3 45 2.7323477079219174 3 46 2.7148229805292914 3 47 1.2714706468463604 3 48 2.464603461475356 3 49 2.9992298507819903 3 50 2.4447675679984933 3 51 1.9517966573030314 3 52 1.0788502157045947 3 53 1.6153787348636186 3 54 1.5589206373318092 3 55 1.5226219228014735 3 56 0.15087705136876117 4 1 -0.21532200560623238 4 2 -0.09405032650223433 4 3 -0.5799140609479991 4 4 -0.17086860311164997 4 5 0.15664977237194222 4 6 0.03483578953416355 4 7 0.026179127920961776 4 8 0.11244588923472065 4 9 0.05582254909288764 4 10 -0.6925453900043728 4 11 0.2452438905665062 4 12 0.12877434909831464 4 13 0.38728481799449455 4 14 -0.25136159273941416 4 15 0.5952529873371866 4 16 -0.9534463475094519 4 17 -0.5557618106524593 4 18 0.4750861344450037 4 19 1.2088703136338665 4 20 0.30860675760145284 4 21 -0.14481037295075083 4 22 0.9313040412274027 4 23 1.0481824883026682 4 24 1.0947266256866932 4 25 0.7106360736279882 4 26 -0.632973338802163 4 27 1.137906810500585 4 28 0.07074985033804637 4 29 -0.47892328438383913 4 30 -0.3940691200858417 4 31 1.5560972411894909 4 32 2.4046183962073187 4 33 0.43979626224777135 4 34 0.12076162659885625 4 35 0.6328533345398313 4 36 0.2436597189307257 4 37 0.371751377500523 4 38 1.095466470462835 4 39 -0.08443757618602277 4 40 -0.511523715366156 4 41 1.5745095112725238 4 42 0.6934718435940326 4 43 1.7979293120618074 4 44 1.2886125775483563 4 45 0.5418715174457276 4 46 1.855515621221933 4 47 0.1765655519412661 4 48 1.4106574075293032 4 49 0.7483140998662385 4 50 0.13141425464517908 4 51 1.7425059480123224 4 52 2.205390342244719 4 53 1.969857589342471 4 54 2.406905985317156 4 55 3.0447664449459957 4 56 0.5260019264936364 5 1 -0.3479130987236374 5 2 -0.18480606199481286 5 3 -0.2934768139844408 5 4 -0.41344620095240714 5 5 -0.666562103471513 5 6 -0.18952318482480734 5 7 -0.1823228963705361 5 8 -0.33593467756689677 5 9 -0.5396565332282961 5 10 1.0156192523707972 5 11 -2.632286473805962 5 12 -1.8492958263402812 5 13 -1.5634574222214255 5 14 -0.1737637519836781 5 15 -1.301170764349724 5 16 -0.09480937045142213 5 17 -0.6218886662529961 5 18 0.4346003044854925 5 19 -1.8478098483094527 5 20 -0.04969283754024545 5 21 -0.09825166849731204 5 22 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3.022719398095113 6 48 3.359375356247252 6 49 3.171391022943162 6 50 3.3878245110554346 6 51 2.4219931274995012 6 52 3.0130826499370293 6 53 2.8160114354963177 6 54 2.3171623955735683 6 55 2.5704074705870212 6 56 0.7311301316218426 7 1 0.10519081490659232 7 2 -0.02994776239967223 7 3 -0.31068329171723263 7 4 0.02143908919603632 7 5 0.00280361852578892 7 6 0.13739989209827286 7 7 0.3466919484337794 7 8 -0.0029387261498925454 7 9 -0.6236646303942877 7 10 -1.3848530823120626 7 11 -0.2675766222540048 7 12 0.1031333234572891 7 13 0.16933610004577915 7 14 -0.418028259406082 7 15 0.12089401297820856 7 16 0.16193826787516485 7 17 0.34167408678343847 7 18 -0.31978566042678835 7 19 1.1063062110697643 7 20 0.9239913729860696 7 21 1.7269844988441214 7 22 0.6620732719966327 7 23 1.3302337703539528 7 24 0.9793420103020765 7 25 0.8132001761920904 7 26 1.2131805073516837 7 27 2.1635478361416105 7 28 0.9169036964918931 7 29 0.9569741515135952 7 30 1.7982385722218517 7 31 2.3894305745228266 7 32 2.72513121672014 7 33 2.696206518658027 7 34 2.6335821394193673 7 35 2.9661866678731634 7 36 2.0000699753409847 7 37 2.551238556987702 7 38 3.3903382653346306 7 39 1.595049603301156 7 40 1.8218096179671797 7 41 0.8693813061443194 7 42 0.44988210000429163 7 43 1.7722882864207818 7 44 1.596304885240663 7 45 0.746999722573932 7 46 0.7785925442988564 7 47 0.8304117057874194 7 48 0.5773240741959711 7 49 1.453442304994443 7 50 0.9262860495169747 7 51 1.1271213326277056 7 52 1.2566723935267703 7 53 0.7903704098552922 7 54 1.445367523778696 7 55 0.6345100346895833 7 56 0.2695916700833809 8 1 0.16524479601320508 8 2 0.18361499063676945 8 3 0.35126002812082646 8 4 0.3760274832581203 8 5 0.09659579126532947 8 6 -0.005312658509019741 8 7 0.3703086825768267 8 8 0.3088021645383634 8 9 0.43402767729801894 8 10 1.3314087260550096 8 11 -0.6059706843322772 8 12 0.11123048944919134 8 13 0.9628583672522559 8 14 -0.7000795414573631 8 15 0.40935555143974867 8 16 0.2341379979696292 8 17 0.6675850179575278 8 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0.4534286836882053 115 39 0.8652925182809135 115 40 0.4169513183720355 115 41 0.6986660564816987 115 42 -0.3858129067433467 115 43 -0.4682650199219971 115 44 -0.09566542515069898 115 45 0.42783642972642966 115 46 0.8993752703447377 115 47 1.105715349512117 115 48 0.4278638852620986 115 49 0.9146433846165749 115 50 0.6874196527558425 115 51 0.0613318589434968 115 52 0.23845377004499113 115 53 0.15946622631953034 115 54 -0.12716958823209978 115 55 -0.5132632446626424 115 56 -0.43148795204887413 116 1 -0.02496173296747628 116 2 0.057199267785335906 116 3 0.1493946050695243 116 4 -0.05418570794774524 116 5 -0.48534957848816873 116 6 0.3262995999853189 116 7 -0.37985787305274954 116 8 -0.2098520659746228 116 9 -0.11344067713235972 116 10 0.485805794675926 116 11 -1.3832858075834444 116 12 -0.4893691108172966 116 13 -0.7566619525345502 116 14 0.28093311677048405 116 15 0.8808745387815229 116 16 1.6082219420069421 116 17 0.843134651535145 116 18 1.4565856475900887 116 19 0.3120835560876145 116 20 1.03158630969493 116 21 0.8707689844007582 116 22 1.1774903443757303 116 23 1.9154333808700166 116 24 1.1059242887830898 116 25 2.022223220658173 116 26 2.6747189688901436 116 27 2.1236257329283674 116 28 2.123817036316627 116 29 2.4319822657622154 116 30 1.9541944307093573 116 31 1.8889437196055887 116 32 2.7444431284371156 116 33 1.7940643570221937 116 34 1.9951530579523116 116 35 0.8092570995511892 116 36 2.8157142466814555 116 37 3.263345015929602 116 38 3.2207504691645568 116 39 3.5030340239438047 116 40 2.8709819646078785 116 41 3.1160544642098813 116 42 2.9088240019841685 116 43 4.394326585674271 116 44 3.0240880725175217 116 45 5.065078268500578 116 46 3.1065704735426074 116 47 3.5271010923502253 116 48 3.1302289752021366 116 49 4.161978494543291 116 50 3.8513103922628353 116 51 3.677754244020111 116 52 4.189486414948387 116 53 3.353336979150974 116 54 3.7017777801889515 116 55 2.4271098399476188 116 56 0.9169464250330712 117 1 -0.4978481214656796 117 2 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0.12400319176014563 119 5 -0.3048886891665177 119 6 -0.17029241559404085 119 7 -0.3071542054123739 119 8 -0.028579751790921648 119 9 0.05582254909288764 119 10 0.5125828151238352 119 11 -0.7803971350745194 119 12 -0.2558410355170686 119 13 -0.5101510794413997 119 14 -0.6359769773547974 119 15 -1.2509008588166601 119 16 0.008092114029011555 119 17 0.572443317552672 119 18 -0.896708737349865 119 19 -0.303950199186648 119 20 -0.883700934706237 119 21 -0.9396821678225464 119 22 -0.5943369844136228 119 23 -0.6569457168255362 119 24 -0.495016964056898 119 25 -0.5201331571412418 119 26 0.4824112765824502 119 27 0.43277860537238055 119 28 0.6604934400816376 119 29 1.2005638951033397 119 30 0.452084726068005 119 31 0.2612254463176953 119 32 0.4174389090278332 119 33 0.36287318532469115 119 34 0.5566590624962906 119 35 0.9661866678731634 119 36 1.3077622830332913 119 37 0.6794436851928296 119 38 1.4287998037961671 119 39 1.2488957571473094 119 40 1.3987326948902563 119 41 1.8437402805032903 119 42 1.3088564589786493 119 43 1.9517754659079642 119 44 1.4937407826765607 119 45 1.5546920302662386 119 46 0.7529515186578308 119 47 0.9073347827104996 119 48 0.9362984331703288 119 49 1.504724356276494 119 50 1.1442347674656936 119 51 0.37071107621745014 119 52 0.6284672653216425 119 53 0.5980627175475988 119 54 -0.14437606596489516 119 55 0.07040747058702124 119 56 0.41061731110902144 120 1 -0.17686046714469228 120 2 -0.020332377784285427 120 3 -0.22702944556338878 120 4 -0.9404198851629317 120 5 -0.08629894557677176 120 6 -0.5145231848248102 120 7 -0.6757439490021184 120 8 -0.2990925723037421 120 9 -0.6409723227019839 120 10 -2.69030180026078 120 11 0.2538977367203543 120 12 -0.10455898423501608 120 13 -0.3345100538003756 120 14 -1.2875795414573616 120 15 -0.10314444856025062 120 16 0.16834852428542035 120 17 0.4070587021680545 120 18 0.5760476729065438 120 19 1.0627164674800191 120 20 0.5114913729860682 120 21 0.8359588578184791 120 22 0.48002198994535306 120 23 1.2860030011231842 120 24 1.1543420103020772 120 25 1.7260206890126035 120 26 1.2247189688901443 120 27 1.372201682295458 120 28 1.8880575426457398 120 29 1.4201151771546208 120 30 0.21875139273467425 120 31 2.275969036061287 120 32 2.7507722423611654 120 33 2.8894757494272554 120 34 3.197684703521933 120 35 3.043750770437267 120 36 3.3859674112384184 120 37 3.395148813397956 120 38 3.1804023678987328 120 39 3.763318834070386 120 40 3.5564250025825643 120 41 4.156560793323806 120 42 2.8062923564145485 120 43 2.838313927446425 120 44 3.353676680112457 120 45 2.4627048507790583 120 46 2.2138489545552638 120 47 2.143873244248958 120 48 2.0443112536831514 120 49 2.5528012793534174 120 50 2.2032091264400506 120 51 2.589621332627704 120 52 3.236480085834465 120 53 3.052229384214268 120 54 3.339277780188951 120 55 3.4373946500742 120 56 1.2481173111090218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75.0 3 88.0 4 80.0 5 85.0 6 95.0 7 696.0 8 217.0 9 683.0 10 1370.0 11 1051.0 12 1532.0 13 1721.0 14 1805.0 15 2168.0 16 2012.0 17 1873.0 18 1880.0 19 1665.0 20 1797.0 21 1993.0 22 2210.0 23 2445.0 24 2547.0 25 2740.0 26 3059.0 27 3447.0 28 3779.0 29 4299.0 30 4777.0 31 5040.0 32 5156.0 33 5161.0 34 4787.0 35 3165.0 36 1641.0 37 1898.0 38 1886.0 39 1084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 40.90992232370407 45.441242820734814 4.242320777494604 9.406514078066506 11 27.350104259392488 23.924786908434644 30.034021485970708 18.691087346202153 12 19.847086224346704 15.946199714658505 35.35429902325411 28.85241503774068 13 16.380308998012367 25.389296035704266 35.86766983306303 22.362725133220334 14 20.577511675832554 30.93760288755838 28.220761642298832 20.264123794310244 15 22.685868279537114 31.00467033302035 26.181911300254857 20.12755008718768 16 27.782994134647044 32.94596802711964 22.334678746936238 16.936359091297085 17 22.291999463460435 33.437389491141 23.821137219993417 20.44947382540515 18 21.92495762556855 32.11677051958979 25.808772421866426 20.149499432975233 19 21.643274354628264 34.08001755947663 25.939249088492446 18.33745899740266 20 24.40767251576085 32.21554257563379 26.383113636640775 16.99367127196459 21 23.301669369687954 30.816881485726828 25.968514882875855 19.912934261709367 22 21.07137195605253 29.481629616983916 28.68657553623471 20.76042289072884 23 19.928053167489786 31.549295774647888 28.243399792695566 20.27925126516676 24 20.9957686538954 31.786310924677164 25.662443449949397 21.555476971478043 25 21.602273281623493 36.321283965071466 22.865749548758476 19.210693204546565 26 22.265343731312317 35.51422399589939 22.890198806429176 19.33023346635912 27 20.706448108774456 31.901233965165808 24.870927975979786 22.521389950079946 28 21.66524233915273 32.13160786228787 24.404834574533023 21.79831522402637 29 23.608074936189443 30.151557710391657 23.827900785266785 22.41246656815212 30 21.703323558162268 29.931573802541543 25.391006842619745 22.97409579667644 31 24.282571498411144 31.57052065509655 23.554143241261304 20.592764605230997 32 23.863775273207345 31.007505561939226 24.795247292374643 20.333471872478793 33 23.73016067105231 31.492259910493754 25.86510479078526 18.91247462766868 34 23.67967326575282 32.7394563396471 25.253426918891158 18.327443475708925 35 22.984625545811472 32.994532711995014 25.9102973372962 18.11054440489732 36 22.60650340835383 34.16063932640648 24.229295933228084 19.0035613320116 37 22.937642985600508 32.83132898616328 24.531741274055225 19.699286754180992 38 21.357571306157425 33.83523075039784 25.361733382298933 19.445464561145794 39 23.978742163009404 32.107170846394986 24.665703369905955 19.248383620689655 40 24.299489089550228 32.648035383917964 23.657481713816637 19.394993812715178 41 22.653023711782367 32.97101893266344 23.27063292690399 21.105324428650203 42 21.47191135190792 32.08835390593398 24.777828170775056 21.661906571383042 43 24.088307039801908 30.56791576263499 24.24643597004131 21.097341227521788 44 23.528979111503386 31.867215847798374 23.755761498479945 20.8480435422183 45 24.11510121010752 32.097887522528595 22.792197442467785 20.99481382489609 46 22.811384845549192 33.80381865672557 23.12163537591818 20.263161121807055 47 20.644520228661154 32.27262690448735 24.84481954905665 22.23803331779484 48 21.625700997656182 32.95087801106871 23.273735749960117 22.14968524131499 49 22.22672015113041 33.328426501797125 22.90631631889498 21.53853702817748 50 21.25868289929064 32.07579588129894 25.5320193416951 21.133501877715325 51 21.102223094665554 32.24836825833047 25.42572508220052 21.223683564803455 52 22.15852595656924 32.564477124584165 24.199943532646724 21.07705338619987 53 21.75391641703089 32.46942984825419 26.23017237145804 19.546481363256888 54 21.57844978943362 32.227092465038616 24.677397571426816 21.517060174100948 55 20.44734320878473 32.27209079519493 24.754956150047903 22.52560984597244 56 20.77212204589004 32.22743575885619 23.01380631847885 23.986635876774923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 228.0 13 1106.5 14 1757.0 15 2288.5 16 2820.0 17 2266.0 18 1712.0 19 1360.0 20 1008.0 21 920.5 22 687.0 23 541.0 24 535.5 25 530.0 26 542.5 27 555.0 28 632.0 29 761.0 30 813.0 31 1176.0 32 1539.0 33 1612.5 34 1686.0 35 1844.5 36 2003.0 37 2237.5 38 2517.0 39 2562.0 40 2827.5 41 3093.0 42 3050.5 43 3008.0 44 3119.5 45 3231.0 46 3315.0 47 3507.0 48 3615.0 49 3478.5 50 3342.0 51 3461.0 52 3580.0 53 3752.5 54 3796.0 55 3667.0 56 3516.5 57 3366.0 58 3396.0 59 3426.0 60 3414.0 61 3402.0 62 3514.0 63 3565.0 64 3504.0 65 3347.0 66 3190.0 67 2738.0 68 2286.0 69 2027.0 70 1768.0 71 1535.0 72 1145.5 73 989.0 74 877.0 75 765.0 76 638.5 77 512.0 78 439.0 79 306.0 80 246.0 81 200.0 82 154.0 83 130.5 84 107.0 85 81.5 86 56.0 87 44.5 88 24.5 89 16.0 90 10.0 91 4.0 92 5.0 93 6.0 94 6.0 95 6.0 96 13.5 97 10.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.002438816198617191 24 0.0 25 0.013413489092394551 26 0.08291975075298451 27 0.09267501554745326 28 0.11828258563293377 29 0.15120660431426586 30 0.20364115258453547 31 0.22802931457070735 32 0.24510102796102773 33 0.274366822344434 34 0.2780250466423598 35 0.3024132086285317 36 0.3609447973953443 37 0.32680137061470366 38 0.3865523674808248 39 0.41825697806284823 40 0.4731303425317351 41 0.49020205592205546 42 0.5194678503054617 43 0.5219066665040789 44 0.5255648908020047 45 0.5414171960930164 46 0.5609277256819539 47 0.5962905605619032 48 0.6292145792432353 49 0.5950711524625947 50 0.6401892521370127 51 0.6084846415549892 52 0.6633580060238761 53 0.6767714951162705 54 0.6828685356128136 55 0.7231090028899971 56 0.7255478190886144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 82007.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.75689880132184 #Duplication Level Percentage of deduplicated Percentage of total 1 72.75559187008477 34.74581438169913 2 11.479930548462875 10.96491762898289 3 5.137371055050557 7.360347287426682 4 3.281074456133184 6.267757630446182 5 1.9584312123378613 4.676430060848464 6 1.3609437238280053 3.8996671015888884 7 0.8451639260545398 2.825368566098016 8 0.6306812378715146 2.409550404233785 9 0.4544990297211725 1.9534917750923702 >10 1.9482177509958125 15.410879559062032 >50 0.09702788274946379 3.2253344226712355 >100 0.04596057603921969 4.080139500286561 >500 0.00510673067102441 2.180301681563769 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 978 1.1925811211238064 No Hit ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 810 0.9877205604399624 No Hit ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 398 0.485324423524821 No Hit ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 331 0.4036240808711451 No Hit ATGGCGGGGAGCCCAGCTGTCTTGTCTCCCTCTGGCTCTGCTGGGATCCTAGGCCC 313 0.3816747350835904 No Hit ATGGCGGGGTGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT 245 0.2987549843306059 No Hit ATGGCGGGGAACACGGTGTGTTTCTGGGACCGACGACTGGCGGGGTCCATCCTGAC 222 0.2707085980465082 No Hit ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 204 0.24875925225895348 No Hit ATGGCGGGGTGGCATCCTTTCTACGGTATCCTTGCTTTGACAACTGCAGTAGACTC 195 0.23778457936517614 No Hit ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 167 0.20364115258453547 No Hit ATGGCGGGGGGTAAGCAGTCATTGCTCCTGACTTTGGTCACTCAGCAAACCGTTTG 161 0.1963247039886839 No Hit ATGGCGGGGATTTGATTTTGCTTTTGCGTGATAACTTTACTTCCCAATGACTTCTT 145 0.17681417439974637 No Hit ATGGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.1707171339032034 No Hit ATGGCGGGGATTTCTATTTCAGAACAATCTGATCTAATGGTCCTAACAGTCTTCAC 136 0.16583950150596902 No Hit ATGGCGGGGAAGTATTACAAATGCTGATGTGCTATGGTTAGTGAATTGTCAAGAAT 130 0.15852305291011742 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 126 0.15364542051288305 No Hit ATGGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 119 0.1451095638177229 No Hit ATGGCGGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT 112 0.1365737071225627 No Hit ATGGCGGGGATCCTGATGACTACCACACATACTGGCTATTTATATATAAATATATG 101 0.12316021803016816 No Hit ATGGCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 101 0.12316021803016816 No Hit ATGGCGGGGGATTTATTTATTTATTTTATATGTATGATGCACCAGAAGAGGGCATC 98 0.11950199373224238 No Hit ATGGCGGGGTACAGGACTTGGCTTATCTGTATCACTGCCTTTTTATACAAGCCAGA 91 0.11096613703708219 No Hit ATGGCGGGGGGTTTCTAATTTGTCATTGTTGTTTGTTTTTGTTTGTTCTTGTTTTT 91 0.11096613703708219 No Hit ATGGCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG 89 0.10852732083846502 No Hit ATGGCGGGGCTGTAAACGTTATCTTTTTAAATTGAATGTGCACAAATAAAAGTTTG 86 0.10486909654053922 No Hit ATGGCGGGGAGTCATTTAACATGCATGAAATTATACCATATGTGGTCTTGTTTGAA 86 0.10486909654053922 No Hit ATGGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.10121087224261344 No Hit ATGGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.10121087224261344 No Hit ATGGCGGGGCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCTCCCC 83 0.10121087224261344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0012194080993085956 0.0 0.0 0.0 0.0 13 0.0012194080993085956 0.0 0.0 0.0 0.0 14 0.004877632397234382 0.0 0.0 0.0 0.0 15 0.006097040496542978 0.0 0.0 0.0 0.0 16 0.0073164485958515735 0.0 0.0 0.0 0.0 17 0.008535856695160169 0.0 0.0 0.0 0.0 18 0.008535856695160169 0.0 0.0 0.0 0.0 19 0.009755264794468765 0.0 0.0 0.0 0.0 20 0.009755264794468765 0.0 0.0 0.0 0.0 21 0.012194080993085957 0.0 0.0 0.0 0.0 22 0.012194080993085957 0.0 0.0 0.0 0.0 23 0.012194080993085957 0.0 0.0 0.0 0.0 24 0.012194080993085957 0.0 0.0 0.0 0.0 25 0.013413489092394551 0.0 0.0 0.0 0.0 26 0.015852305291011743 0.0 0.0 0.0 0.0 27 0.017071713390320337 0.0 0.0 0.0 0.0 28 0.01951052958893753 0.0 0.0 0.0 0.0 29 0.02194934578755472 0.0 0.0 0.0 0.0 30 0.02194934578755472 0.0 0.0 0.0 0.0 31 0.02194934578755472 0.0 0.0 0.0 0.0 32 0.023168753886863316 0.0 0.0 0.0 0.0 33 0.024388161986171913 0.0 0.0 0.0 0.0 34 0.024388161986171913 0.0 0.0 0.0 0.0 35 0.025607570085480508 0.0 0.0 0.0 0.0 36 0.025607570085480508 0.0 0.0 0.0 0.0 37 0.025607570085480508 0.0 0.0 0.0 0.0 38 0.025607570085480508 0.0 0.0 0.0 0.0 39 0.026826978184789102 0.0 0.0 0.0 0.0 40 0.026826978184789102 0.0 0.0 0.0 0.0 41 0.026826978184789102 0.0 0.0 0.0 0.0 42 0.0280463862840977 0.0 0.0 0.0 0.0 43 0.0280463862840977 0.0 0.0 0.0 0.0 44 0.0280463862840977 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 20 4.0654864E-4 50.23558 50 GAATAGT 15 0.008268461 50.204784 49 CTAGGCC 30 1.0070453E-6 50.204784 49 GTAGACT 40 2.5374902E-9 50.20478 49 ACCGTTT 45 1.2732926E-10 50.20478 49 GTCGCGT 15 0.0083086435 50.143295 43 CCTAGGC 30 1.0156091E-6 50.143295 48 GTGGAAC 15 0.0083086435 50.143295 43 CCCAATG 25 2.0355701E-5 50.143295 43 TATCTTG 15 0.0083086435 50.143295 42 CACAGGT 15 0.0083086435 50.143295 46 GACGCAT 20 4.102583E-4 50.143295 48 CGCGTGC 15 0.0083086435 50.143295 45 GCCACGA 15 0.0083086435 50.143295 43 CATCAGT 20 4.102583E-4 50.143295 42 TCGCGTG 15 0.0083086435 50.143295 44 AGTCGCG 15 0.0083086435 50.143295 42 AACCGTT 45 1.2914825E-10 50.14329 48 CAACTGC 45 1.2914825E-10 50.14329 41 TTATGTG 15 0.008348971 50.08196 37 >>END_MODULE