FastQCFastQC Report
Tue 31 May 2016
SRR212721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495050
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95081.9206140793859208No Hit
GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61861.2495707504292495No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41170.8316331683668317No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT26770.5407534592465407No Hit
GGGTTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22570.4559135440864559No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15090.3048176951823048No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA15040.3038076961923038No Hit
GGGTTTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10030.2026057973942026No Hit
GGGTTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.19593980406019595No Hit
GGGTTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9570.1933138066861933No Hit
GGGTTTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8400.16967983032016967No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7870.15897384102615897No Hit
GGGTTTGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7760.15675184324815675No Hit
GGGTTTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7190.14523785476214524No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA7150.14442985557014443No Hit
GGGTTTGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6680.13493586506413494No Hit
GGGTTTGGGGAATATAGTTTGTACCCATATTCTTAGACAAAAATGGGAAAGGCTTATG6240.12604787395212605No Hit
GGGTTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA6090.12301787698212302No Hit
GGGTTTGGGGAATTTCTCTTCATTCTGGAGGAGTGTGGCTCAGTGTTGGGTTGGTACA5910.11938188061811937No Hit
GGGTTTGGGATCCAGCACTATGAGATACTGAAGACCAAGAATTTAGCATTAGAACATC5330.10766589233410767No Hit
GGGTTTGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.10463589536410464No Hit
GGGTTTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130.10362589637410362No Hit
GGGTTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4970.10039389960610039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGG150.00719013652.05037352
ATTCGAT150.00719013652.05037352
TATTCGA150.00719013652.05037351
CTATTCG150.00719013652.05037350
TGTAGCG353.8540747E-852.0398542
CGAAATT150.0071958952.03984546
TAAACGT150.0071958952.03984543
TACCCGT353.869536E-852.01354635
TCGAGTA203.4464715E-451.99778429
CGAGTCA150.007218940651.99777632
AATTACG251.6542375E-551.99777628
TTAGCCG203.4481913E-451.99252324
AAACCGC150.007221825451.99252326
CGTCGAG150.007221825451.99252326
CAATCGG459.276846E-1151.9925230
TCGGTAG150.007230486351.9767721
CGTAACG150.007233374851.9715222
TTGGGAT85400.051.1499525
GTTTGGG504850.050.9626663
TTTGGGA249950.050.8798984
Produced by FastQC (version 0.11.5)