FastQCFastQC Report
Tue 31 May 2016
SRR212720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497067
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94471.9005486181943279No Hit
GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59181.1905839655418686No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39700.7986850867186918No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT27050.5441922316307459No Hit
GGGTTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22310.44883285351874097No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA14740.29653950071117174No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA14300.2876875753168084No Hit
GGGTTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9930.19977186174097256No Hit
GGGTTTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9830.19776006051498088No Hit
GGGTTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.18971285561101423No Hit
GGGTTTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8660.17422198617087836No Hit
GGGTTTGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470.1703995638414942No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7850.15792639624034585No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6850.13780838398042922No Hit
GGGTTTGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6690.13458950201884254No Hit
GGGTTTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6650.13378478152844586No Hit
GGGTTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA6380.12835291821826836No Hit
GGGTTTGGGGAATATAGTTTGTACCCATATTCTTAGACAAAAATGGGAAAGGCTTATG5970.12010453319170253No Hit
GGGTTTGGGATCCAGCACTATGAGATACTGAAGACCAAGAATTTAGCATTAGAACATC5960.11990335306910337No Hit
GGGTTTGGGGAATTTCTCTTCATTCTGGAGGAGTGTGGCTCAGTGTTGGGTTGGTACA5520.11105142767474004No Hit
GGGTTTGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5230.1052172041193642No Hit
GGGTTTGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5060.10179714203517837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA150.00713472552.15232527
ACTAGCC203.411624E-452.10495421
CCAACGG150.0071604152.10495421
ACCCACG150.0071604152.10495421
CGGTAAA150.00721198652.0104844
ACGTACT150.00721485952.00523841
GATACGT150.007217733752.038
TCGATAC150.007217733752.028
CCGTCGT150.007217733752.040
TACGTAC150.007217733752.040
AGTGCGA307.9914753E-751.99476230
CGATCAT150.007220608651.99476235
TACGAGT353.8866347E-851.98429531
GTGCGAT308.0025893E-751.98429531
TTGCGCT150.007243640351.9529116
ATACTAC203.462923E-451.94768519
GACGTAT150.007252291351.937239
GTTTGGG505550.051.063993
TTGGGAT87500.050.8984875
TTTGGGA251100.050.8720054
Produced by FastQC (version 0.11.5)