FastQCFastQC Report
Tue 31 May 2016
SRR212719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498267
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85381.7135391266128401No Hit
GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54631.096400122825714No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35270.707853419953559No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT24140.4844792049242675No Hit
GGGTTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20100.4033981780852435No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13440.26973490116744636No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA13360.2681293362795449No Hit
GGGTTTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9230.1852420489416317No Hit
GGGTTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8910.17881978939002582No Hit
GGGTTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8760.1758093552252106No Hit
GGGTTTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.16155996684508506No Hit
GGGTTTGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7740.15533840290446688No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7270.14590570918804577No Hit
GGGTTTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6810.13667371108261234No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6430.12904727786508038No Hit
GGGTTTGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6080.12202293148051145No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGTGG5920.11881180170470852No Hit
GGGTTTGGGATCCAGCACTATGAGATACTGAAGACCAAGAATTTAGCATTAGAACATC5500.11038258604322582No Hit
GGGTTTGGGGAATATAGTTTGTACCCATATTCTTAGACAAAAATGGGAAAGGCTTATG5240.10516450015754605No Hit
GGGTTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA5120.10275615282569386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA203.329422E-452.3629959
ACGGTAG150.00702188252.36298810
CGTCTAA150.00720658652.02033628
CGTATAC307.997678E-751.98893434
TATATCG150.00722381651.98893434
CCGACCG150.00722381651.98893443
CGCGTTC401.895387E-951.98893440
CGACTTA150.00722381651.98893433
AGTGCGA401.900844E-951.97324830
CATGCGA150.007238197651.96278852
CGCATAA150.007243956451.9523450
TATCCCG150.00724683751.9471223
CGTCATT203.4837853E-451.8845321
TACGTTC150.00728725551.87410713
ACGTCAA150.00729014851.868919
TTGGGAT86400.051.1508875
TTTGGGA251650.051.0835574
GTTTGGG508650.050.793083
GGTTTGG510400.050.6189272
TTGGGAC37350.050.610555
Produced by FastQC (version 0.11.5)