FastQCFastQC Report
Tue 31 May 2016
SRR212718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497621
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90131.8112177741694984No Hit
GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57951.1645408855333679No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37660.75680085848467No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT30830.6195478084727132No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC26550.5335385765472116No Hit
GGGTTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21820.4384863179005709No Hit
GGGTTTGGGATGTGTGTGTGTGTGAGTGTATGTAGCTCAGTGGATGACTTTGTGTG16890.33941493626675723No Hit
GGGTTTGGGGGTGTGCGTGTCTGTGTCCTGGTGTGAGAGTGTGTGTGTGTCCTGTG14040.2821424336995424No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9840.1977408509689101No Hit
GGGTTTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9210.1850806135593152No Hit
GGGTTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9190.18467870126059793No Hit
GGGTTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9110.18307105206572874No Hit
GGGTTTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8250.16578882322088498No Hit
GGGTTTGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7820.15714770879846307No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGT7100.1426788660446404No Hit
GGGTTTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7030.14127217299912986No Hit
GGGTTTGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6710.13484157621965312No Hit
GGGTTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC6670.13403775162221854No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT6190.1243918564530034No Hit
GGGTTTGGGGAATATAGTTTGTACCCATATTCTTAGACAAAAATGGGAAAGGCTTA6000.12057368961518908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG204.1071506E-450.18230445
CCGATCG150.00840786950.0305432
TGCGATC301.0422664E-650.0305432
GTGCGAT402.6684575E-950.0204631
CGACGGT150.00842127850.0103830
AGTGCGA402.6720954E-950.0103830
ACGACGG150.00842127850.0103829
GTCGCAA150.00851558549.86967510
TTGGGAT94650.048.868595
GTTTGGG508250.048.8246963
TTGGGAC39150.048.7869385
GGTTTGG510150.048.6428532
TTTGGGA261850.048.631744
GGGTTTG510650.048.595221
TTTGGGG199450.048.3194544
TTGGGGC38450.048.248425
TTGGGAG69550.047.826135
TTTGGGC34650.047.7108154
TGGGATC25600.047.434636
TTGGGGG60300.047.2645385
Produced by FastQC (version 0.11.5)