FastQCFastQC Report
Tue 31 May 2016
SRR212717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446993
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81181.8161358231560671No Hit
GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49851.1152299924159887No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33930.7590722897226578No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT27420.613432425116277No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC23970.5362500083893931No Hit
GGGTTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18880.4223779790734978No Hit
GGGTTTGGGATGTGTGTGTGTGTGAGTGTATGTAGCTCAGTGGATGACTTTGTGTG18770.4199170904242348No Hit
GGGTTTGGGGGTGTGCGTGTCTGTGTCCTGGTGTGAGAGTGTGTGTGTGTCCTGTG12920.28904255771343174No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9220.20626721223822295No Hit
GGGTTTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8850.1979896776907021No Hit
GGGTTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7870.1760653969972684No Hit
GGGTTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7740.17315707404813946No Hit
GGGTTTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6990.1563782878031647No Hit
GGGTTTGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6820.15257509625430377No Hit
GGGTTTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5900.13199311846046807No Hit
GGGTTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC5710.12774249261174112No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5640.12617647256221015No Hit
GGGTTTGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.12416301821281317No Hit
GGGTTTGGGGAATATAGTTTGTACCCATATTCTTAGACAAAAATGGGAAAGGCTTA4980.11141114066663235No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGT4970.11118742351669936No Hit
GGGTTTGGGCACTCTCTGTAATGTGCTACCCAGTAATACATTCCAGAGTAGCTTGA4610.10313360611911147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA150.00828221250.22064647
GCGCCAA204.1026334E-450.19236842
AACGCGT150.00830067450.19236443
CTCGTTT252.0453597E-550.16411239
TGCGATC204.1599537E-450.0514432
AATATCG150.008419557550.0121229
CGAATAT150.00844947549.96726226
CGCGATC402.6939233E-949.9616625
CGCGAAC150.0084532249.9616625
CTCGATG204.1969714E-449.9616625
TCGAATA150.0084532249.9616625
ACGCGGA301.0552321E-649.93926624
ACGCGAT402.7048372E-949.93926224
TAGGTGC150.00848322749.91689323
TCGACGT150.0084982649.8945421
CTTAACG204.2342505E-449.872219
GAATCGC252.1200192E-549.86104217
ACGGCGG150.00852084649.86104218
AATCGCG252.1200192E-549.86104218
TCGATCT150.00852461549.85546516
Produced by FastQC (version 0.11.5)