FastQCFastQC Report
Tue 31 May 2016
SRR212716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81439
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC31943.9219538550326014No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC13781.6920639988212038No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9501.1665172705951694No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9241.1345915347683542No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9241.1345915347683542No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8451.0375864143714928No Hit
CCTAGAGGGGTGCGATGGCTTAGCAGGTCAAGGCACTCGCTACCAAGCTGTCGTGTCG6600.8104225248345387No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAG5430.6667567136138706No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5100.6262355873721436No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5040.6188681098736478No Hit
CCTAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4470.5488770736379376No Hit
CCTAGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT4400.5402816832230258No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4230.5194071636439545No Hit
CCTAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3630.4457323886589963No Hit
CCTAGAGGGGAGCCAAGGCCAAGGAATTCACCAATGTTTATATCAAAAACTTTGGAGA3330.4088950011665172No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA3140.3855646557546139No Hit
CCTAGAGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC2880.3536389199277987No Hit
CCTAGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2860.3511830940949668No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2720.3339923132651432No Hit
CCTAGAGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT2630.32294109701739954No Hit
CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGCCC2500.306978229103992No Hit
CCTAGAGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT2460.30206657743832804No Hit
CCTAGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC2410.29592701285624823No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC2390.2934711870234163No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCG2380.29224327410700035No Hit
CCTAGAGGGGTTCAGATGTGACTGGAATCCCTTTCTGCTAATGTTCATGTGCCTGTCA2230.27382458036076085No Hit
CCTAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2220.27259666744434485No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.27259666744434485No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAGA2220.27259666744434485No Hit
CCTAGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2190.2689129286950969No Hit
CCTAGAGGGGAGCAATGCGCGTGGTACGCGGCAGTGACACCCGCCACAGCGCAGCTCA2120.26031753828018517No Hit
CCTAGAGGGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGACCC2050.25172214786527336No Hit
CCTAGAGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG2010.2468104961996095No Hit
CCTAGAGGGGAATTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGG2000.24558258328319355No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGA1980.2431267574503616No Hit
CCTAGAGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCACTGCTCTTCCGAA1920.23575927995186582No Hit
CCTAGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA1920.23575927995186582No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCAAA1920.23575927995186582No Hit
CCTAGAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG1900.2333034541190339No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCACTTCCGAA1760.2161126732892103No Hit
CCTAGAGGGGGTACTTGAGAGATGGCTCAGAGGTTAAGACCACTGACTGCTCTTCCAG1720.21120102162354645No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1710.2099731087071305No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1630.20014980537580276No Hit
CCTAGAGGGGGGACTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA1580.19401024079372292No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1570.19278232787730695No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1570.19278232787730695No Hit
CCTAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1550.190326502044475No Hit
CCTAGAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1530.18787067621164308No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1510.18541485037881114No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA1470.18050319871314727No Hit
CCTAGAGGGGAGTGTGAGCAGGCATTGGCTGCTGAGCCCAAGGCCAAGGACCCTTTCG1460.1792752857967313No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1450.17804737288031533No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1440.17681945996389936No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1390.17067989538181952No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA1380.16945198246540355No Hit
CCTAGAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1380.16945198246540355No Hit
CCTAGAGGGGGGAATTCCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1370.16822406954898758No Hit
CCTAGAGGGCACTGTCCTTAAATCCAAGATGGTTGCCGTGTAGTATCAAATAAGTTTG1350.16576824371615564No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTCCA1340.16454033079973968No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG1330.1633124178833237No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG1330.1633124178833237No Hit
CCTAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1290.15840076621765983No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1270.15594494038482792No Hit
CCTAGAGGGAGCCATTCCTAGAATTCCACTGTCACCCATTCGAAGAACTGGTCCAGTG1250.153489114551996No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA1190.14612163705350018No Hit
CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC1180.14489372413708418No Hit
CCTAGAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGC1160.14243789830425227No Hit
CCTAGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1160.14243789830425227No Hit
CCTAGAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1130.13875415955500436No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA1120.1375262466385884No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA1120.1375262466385884No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1110.13629833372217243No Hit
CCTAGAGGGGGTGCAGCGGGAGGTTTTTAGGAGGGCTGTTTTGCCAAGGGTCAATCGA1110.13629833372217243No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCC1100.13507042080575646No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1080.13261459497292452No Hit
CCTAGAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC1070.13138668205650855No Hit
CCTAGAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1060.13015876914009258No Hit
CCTAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1050.12893085622367662No Hit
CCTAGAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1040.12770294330726065No Hit
CCTAGAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1040.12770294330726065No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1030.12647503039084468No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG1030.12647503039084468No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1030.12647503039084468No Hit
CCTAGAGGGGGGATGCAGGCATGCTTCCCACTGTTCAGAGCTCTGGCTTCCATCTGGC1030.12647503039084468No Hit
CCTAGAGGGGGGAATTGGCCTGGAATTACATCTGGAGTGGGAAAGGAGTTGTAGGGAC1020.1252471174744287No Hit
CCTAGAGGGAGGCTATTTGAATTCATTTAAATGAAATACTGAAGAAATTTGTTCCTCA1010.12401920455801276No Hit
CCTAGAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT1010.12401920455801276No Hit
CCTAGAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1000.12279129164159677No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGAA1000.12279129164159677No Hit
CCTAGAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC990.1215633787251808No Hit
CCTAGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC970.11910755289234888No Hit
CCTAGAGGGGGGGCTGAAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAG960.11787963997593291No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCCACCCGACTGCT960.11787963997593291No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAA960.11787963997593291No Hit
CCTAGAGGGGGCGGTGGCTTCTTGGGCGAACAGCGTGCGGCTGCGTCATCGCCAGTGG960.11787963997593291No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG960.11787963997593291No Hit
CCTAGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT950.11665172705951694No Hit
CCTAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA940.11542381414310096No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCGA940.11542381414310096No Hit
CCTAGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA920.11296798831026904No Hit
CCTAGAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA920.11296798831026904No Hit
CCTAGAGGGGAGCTGGAGAGATGGCTCAGCGGGTAGGAGCACTGACTGCTCTTCCAGA900.1105121624774371No Hit
CCTAGAGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA890.10928424956102115No Hit
CCTAGAGGGGGGGCTGGAGTGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAG890.10928424956102115No Hit
CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGTGCCC890.10928424956102115No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAAAG860.10560051081177323No Hit
CCTAGAGGGGTTGTTAAAAGACCATTTTTAGTCAAGCTGGAGGGTATGAGCCCCTGTA860.10560051081177323No Hit
CCTAGAGGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCA850.10437259789535726No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.10437259789535726No Hit
CCTAGAGGGTGCTCCTAGAGGGGCTCTCCTTGCCCTCTCGTCTCCTAGGGCCGGGGTT840.1031446849789413No Hit
CCTAGAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGGGC820.10068885914610935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATT251.6310982E-552.05596552
GAAACAC203.410465E-452.05596551
GATATGG251.6310982E-552.05596551
AAACACC203.410465E-452.05596552
TCTGGTA251.6310982E-552.05596550
GTCGTGT1000.052.05596550
GTCAATC251.6310982E-552.05596550
CCTGTCA203.410465E-452.05596552
TCGTGTC1000.052.05596551
TGATATG251.6310982E-552.05596550
TCAATCG251.6310982E-552.05596551
CCCGTCA900.052.05596551
CCCCGTC900.052.05596550
ATTAAAG353.7915015E-852.05596551
TGTAGGG203.410465E-452.05596550
CGTGTCG1000.052.05596552
GTAGGGA203.410465E-452.05596551
GATTAAA353.7915015E-852.05596550
TAGGGAC203.410465E-452.05596552
GCCCCAA150.007165613652.0559652
Produced by FastQC (version 0.11.5)