FastQCFastQC Report
Tue 31 May 2016
SRR212715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83104
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC30613.6833365421640356No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC12901.5522718521370813No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10071.211734693877551No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9591.153975741239892No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9521.1455525606469004No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8170.9831055063534847No Hit
CCTAGAGGGGTGCGATGGCTTAGCAGGTCAAGGCACTCGCTACCAAGCTGTCGTGTCG6660.8014054678475163No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAG5810.6991239892183289No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5420.652194840200231No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4840.5824027724297266No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4780.5751829033500192No Hit
CCTAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4460.5366769349249133No Hit
CCTAGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT3920.4716981132075472No Hit
CCTAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3870.46568155564112435No Hit
CCTAGAGGGGAGCCAAGGCCAAGGAATTCACCAATGTTTATATCAAAAACTTTGGAGA3260.39227955333076625No Hit
CCTAGAGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC3030.3646033885252214No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA3010.36219676549865226No Hit
CCTAGAGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT2930.3525702733923758No Hit
CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGCCC2830.34053715825953024No Hit
CCTAGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC2740.3297073546399692No Hit
CCTAGAGGGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGACCC2730.32850404312668463No Hit
CCTAGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2590.3116576819407008No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2380.2863881401617251No Hit
CCTAGAGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT2380.2863881401617251No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC2270.2731517135155949No Hit
CCTAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2260.27194840200231035No Hit
CCTAGAGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG2250.2707450904890258No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCAAA2250.2707450904890258No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGA2250.2707450904890258No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAGA2190.26352522140931844No Hit
CCTAGAGGGGAGCAATGCGCGTGGTACGCGGCAGTGACACCCGCCACAGCGCAGCTCA2180.2623219098960339No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCG2150.2587119753561802No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.25510204081632654No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2100.2526954177897574No Hit
CCTAGAGGGGGTACTTGAGAGATGGCTCAGAGGTTAAGACCACTGACTGCTCTTCCAG2060.24788217173661917No Hit
CCTAGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1990.2394589911436273No Hit
CCTAGAGGGGTTCAGATGTGACTGGAATCCCTTTCTGCTAATGTTCATGTGCCTGTCA1930.23223912206391992No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCACTTCCGAA1820.21900269541778977No Hit
CCTAGAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1770.21298613785136694No Hit
CCTAGAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1750.21057951482479784No Hit
CCTAGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA1730.2081728917982287No Hit
CCTAGAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG1710.2057662687716596No Hit
CCTAGAGGGGAATTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGG1690.20335964574509047No Hit
CCTAGAGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCACTGCTCTTCCGAA1670.20095302271852136No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG1650.19854639969195226No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1620.19493646515209856No Hit
CCTAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1580.19012321909896032No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1560.18771659607239122No Hit
CCTAGAGGGGAGTGTGAGCAGGCATTGGCTGCTGAGCCCAAGGCCAAGGACCCTTTCG1520.18290335001925298No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1520.18290335001925298No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1520.18290335001925298No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA1500.18049672699268388No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1480.17809010396611474No Hit
CCTAGAGGGGGGACTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA1420.1708702348864074No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1410.16966692337312284No Hit
CCTAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1390.16726030034655373No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1380.16605698883326916No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1340.1612437427801309No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA1340.1612437427801309No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA1310.15763380824027723No Hit
CCTAGAGGGAGCCATTCCTAGAATTCCACTGTCACCCATTCGAAGAACTGGTCCAGTG1310.15763380824027723No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1270.152820562187139No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTCCA1260.15161725067385445No Hit
CCTAGAGGGCACTGTCCTTAAATCCAAGATGGTTGCCGTGTAGTATCAAATAAGTTTG1250.1504139391605699No Hit
CCTAGAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1220.14680400462071622No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1200.14439738159414708No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCCACCCGACTGCT1180.14199075856757798No Hit
CCTAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA1140.13717751251443974No Hit
CCTAGAGGGGGGAATTCCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1130.13597420100115518No Hit
CCTAGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1120.13477088948787064No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG1100.1323642664613015No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1090.13116095494801694No Hit
CCTAGAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGC1090.13116095494801694No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGAA1080.1299576434347324No Hit
CCTAGAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1060.12755102040816327No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAA1050.1263477088948787No Hit
CCTAGAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1050.1263477088948787No Hit
CCTAGAGGGGGTGCAGCGGGAGGTTTTTAGGAGGGCTGTTTTGCCAAGGGTCAATCGA1050.1263477088948787No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA1040.12514439738159414No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAAAG1030.12394108586830958No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1010.12153446284174046No Hit
CCTAGAGGGGGTTCTGCTTGGAGACCACAAGCAGCTTCGGCCTGTGGTCAAGAGTGAA1010.12153446284174046No Hit
CCTAGAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1000.1203311513284559No Hit
CCTAGAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1000.1203311513284559No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.1203311513284559No Hit
CCTAGAGGGGGGATGCAGGCATGCTTCCCACTGTTCAGAGCTCTGGCTTCCATCTGGC1000.1203311513284559No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA990.11912783981517135No Hit
CCTAGAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC980.1179245283018868No Hit
CCTAGAGGGGGGAATTGGCCTGGAATTACATCTGGAGTGGGAAAGGAGTTGTAGGGAC970.11672121678860223No Hit
CCTAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC960.11551790527531768No Hit
CCTAGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG960.11551790527531768No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG950.11431459376203311No Hit
CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.11431459376203311No Hit
CCTAGAGGGGAGCTGGAGAGATGGCTCAGCGGGTAGGAGCACTGACTGCTCTTCCAGA940.11311128224874856No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG940.11311128224874856No Hit
CCTAGAGGGAGGCTATTTGAATTCATTTAAATGAAATACTGAAGAAATTTGTTCCTCA930.11190797073546399No Hit
CCTAGAGGGGTTGTTAAAAGACCATTTTTAGTCAAGCTGGAGGGTATGAGCCCCTGTA930.11190797073546399No Hit
CCTAGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT920.11070465922217944No Hit
CCTAGAGGGTGCTCCTAGAGGGGCTCTCCTTGCCCTCTCGTCTCCTAGGGCCGGGGTT910.10950134770889489No Hit
CCTAGAGGGCAGCACCACATGCTCCATGTCAAGTGTGTACGTGGAGACCACTGGCTCC900.10829803619561032No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC890.10709472468232577No Hit
CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC890.10709472468232577No Hit
CCTAGAGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCAAAGG860.10348479014247208No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT850.10228147862918753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGG150.00717660952.0367552
CTGAGTC307.866438E-752.0367546
CCAATGG150.00717660952.0367523
CGGAGAC307.866438E-752.0367552
TTACCAG150.00717660952.0367546
AATTCAC307.866438E-752.0367525
GTCAATC150.00717660952.0367550
CACGCGA150.00717660952.0367552
CAACTGG150.00717660952.0367551
TATAATA150.00717660952.0367523
TGATCCT150.00717660952.0367551
ATGGCCT150.00717660952.0367526
TTACACA150.00717660952.0367527
GGATTAA307.866438E-752.0367549
AACCCCA307.866438E-752.0367527
TCAATCG150.00717660952.0367551
GGTCAAT150.00717660952.0367549
ATAATAT150.00717660952.0367524
TCAAAGG150.00717660952.0367552
GACGCAA150.00717660952.0367526
Produced by FastQC (version 0.11.5)