FastQCFastQC Report
Tue 31 May 2016
SRR212714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85232
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC30533.581987985733058No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC11831.387976346911958No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9661.133377135348226No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9281.0887929416181714No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9151.0735404542894689No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7610.8928571428571428No Hit
CCTAGAGGGGTGCGATGGCTTAGCAGGTCAAGGCACTCGCTACCAAGCTGTCGTGTCG6070.7121738314248169No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAG5140.603059883611789No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4560.5350103247606532No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA4500.5279707152243289No Hit
CCTAGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT4470.5244509104561668No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4320.5068518866153557No Hit
CCTAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4250.4986390088229773No Hit
CCTAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3330.39069832926600334No Hit
CCTAGAGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC3180.37309930542519243No Hit
CCTAGAGGGGAGCCAAGGCCAAGGAATTCACCAATGTTTATATCAAAAACTTTGGAGA3060.3590200863525436No Hit
CCTAGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC2830.3320349164633002No Hit
CCTAGAGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT2640.30974281959827293No Hit
CCTAGAGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT2600.3050497465740567No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA2580.30270321006194856No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGA2520.2956636005256242No Hit
CCTAGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2440.28627745447719166No Hit
CCTAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2400.28158438145297543No Hit
CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGCCC2360.27689130842875914No Hit
CCTAGAGGGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGACCC2310.27102496714848884No Hit
CCTAGAGGGGTTCAGATGTGACTGGAATCCCTTTCTGCTAATGTTCATGTGCCTGTCA2240.2628120893561104No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2090.24521306551529942No Hit
CCTAGAGGGGAGCAATGCGCGTGGTACGCGGCAGTGACACCCGCCACAGCGCAGCTCA2060.24169326074713723No Hit
CCTAGAGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG2010.23582691946686687No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.23582691946686687No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCG1920.22526750516238034No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAGA1890.22174770039421815No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCAAA1880.22057443213816408No Hit
CCTAGAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG1850.21705462737000186No Hit
CCTAGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1830.21470809085789375No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1740.20414867655340718No Hit
CCTAGAGGGGGTACTTGAGAGATGGCTCAGAGGTTAAGACCACTGACTGCTCTTCCAG1690.19828233527313682No Hit
CCTAGAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1670.1959357987610287No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCACTTCCGAA1630.19124272573681245No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG1620.1900694574807584No Hit
CCTAGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA1550.18185657968837995No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1540.18068331143232588No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1520.17833677492021777No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA1520.17833677492021777No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1510.1771635066641637No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1510.1771635066641637No Hit
CCTAGAGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCACTGCTCTTCCGAA1490.17481697015205555No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1490.17481697015205555No Hit
CCTAGAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1470.17247043363994743No Hit
CCTAGAGGGGAGTGTGAGCAGGCATTGGCTGCTGAGCCCAAGGCCAAGGACCCTTTCG1430.16777736061573117No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1430.16777736061573117No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1420.16660409235967713No Hit
CCTAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1370.1607377510794068No Hit
CCTAGAGGGGGGAATTCCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1370.1607377510794068No Hit
CCTAGAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1320.15487140979913647No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1320.15487140979913647No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG1310.1536981415430824No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA1300.15252487328702835No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTCCA1300.15252487328702835No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1290.15135160503097428No Hit
CCTAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1260.1478318002628121No Hit
CCTAGAGGGGGGACTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA1230.1443119954946499No Hit
CCTAGAGGGGAATTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGG1230.1443119954946499No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA1210.14196545898254176No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1210.14196545898254176No Hit
CCTAGAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGC1200.14079219072648771No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1190.13961892247043364No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAA1150.13492584944621738No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1130.13257931293410927No Hit
CCTAGAGGGAGCCATTCCTAGAATTCCACTGTCACCCATTCGAAGAACTGGTCCAGTG1130.13257931293410927No Hit
CCTAGAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1120.1314060446780552No Hit
CCTAGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1110.13023277642200112No Hit
CCTAGAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1090.127886239909893No Hit
CCTAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1090.127886239909893No Hit
CCTAGAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1050.12319316688567675No Hit
CCTAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA1050.12319316688567675No Hit
CCTAGAGGGGGTGCAGCGGGAGGTTTTTAGGAGGGCTGTTTTGCCAAGGGTCAATCGA1040.12201989862962268No Hit
CCTAGAGGGCACTGTCCTTAAATCCAAGATGGTTGCCGTGTAGTATCAAATAAGTTTG1020.11967336211751456No Hit
CCTAGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1020.11967336211751456No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA980.11498028909329829No Hit
CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGTGCCC970.11380702083724423No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA970.11380702083724423No Hit
CCTAGAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT930.10911394781302798No Hit
CCTAGAGGGCAGCACCACATGCTCCATGTCAAGTGTGTACGTGGAGACCACTGGCTCC930.10911394781302798No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA920.1079406795569739No Hit
CCTAGAGGGAGGCTATTTGAATTCATTTAAATGAAATACTGAAGAAATTTGTTCCTCA910.10676741130091984No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAAAG910.10676741130091984No Hit
CCTAGAGGGGGGGCTGGAGTGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAAG900.10559414304486578No Hit
CCTAGAGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA890.10442087478881171No Hit
CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC890.10442087478881171No Hit
CCTAGAGGGGGCGGTGGCTTCTTGGGCGAACAGCGTGCGGCTGCGTCATCGCCAGTGG880.10324760653275765No Hit
CCTAGAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT880.10324760653275765No Hit
CCTAGAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT860.10090107002064953No Hit
CCTAGAGGGTGCTCCTAGAGGGGCTCTCCTTGCCCTCTCGTCTCCTAGGGCCGGGGTT860.10090107002064953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG2050.052.470316
AGGGGAT503.6379788E-1252.470316
GGGTACT251.5568115E-552.470319
AGGGCTC1000.052.470316
GATGCCA203.2802418E-452.470319
AGGGCCT2050.052.470316
AGGGTCC203.2802418E-452.470316
AGGGAAT203.2802418E-452.470316
AGGCTAT203.2802418E-452.4703110
GGGACAT650.052.470317
GGTACTT251.5568115E-552.4703110
GGGTACG150.006945315352.4703067
GCCTAAT150.006945315352.47030610
GAAGCAT700.052.4703069
AGGGCGT150.006945315352.4703066
GGACATG353.563946E-852.4703068
AGGGTAC307.430026E-752.4703066
GGACATA150.006945315352.4703068
AGGGATA353.563946E-852.4703066
GGCCTAA150.006945315352.4703069
Produced by FastQC (version 0.11.5)