FastQCFastQC Report
Tue 31 May 2016
SRR212713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99493
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC26732.6866211693284954No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC21242.13482355542601No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC13461.3528589951051833No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13071.3136602575055532No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12431.2493341240087241No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10911.0965595569537556No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC10581.063391394369453No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8840.8885047189249494No Hit
CCTAGAGGGGTGCGATGGCTTAGCAGGTCAAGGCACTCGCTACCAAGCTGTCGTGT7220.7256791935111013No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT6660.669393826701376No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA5890.5920014473380036No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCA5850.5879810639944518No Hit
CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4980.5005377262722001No Hit
CCTAGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC4950.4975224387645362No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC4380.44023197611892295No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTA4270.42917592192415543No Hit
CCTAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4120.4140994843858362No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC3570.3588192134119988No Hit
CCTAGAGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3550.35680902174022294No Hit
CCTAGAGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC3530.35479883006844704No Hit
CCTAGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC3450.3467580633813434No Hit
CCTAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3420.3437427758736795No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.33972239253012776No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT3340.33570200918657594No Hit
CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3270.3286663383353603No Hit
CCTAGAGGGGAGCCAAGGCCAAGGAATTCACCAATGTTTATATCAAAAACTTTGGA3100.31157970912526506No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC3020.3035389424381615No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2940.29549817575105786No Hit
CCTAGAGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC2860.28745740906395423No Hit
CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGC2850.2864523132280663No Hit
CCTAGAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2670.26836058818208314No Hit
CCTAGAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC2610.2623300131667554No Hit
CCTAGAGGGGAGCAATGCGCGTGGTACGCGGCAGTGACACCCGCCACAGCGCAGCT2480.24926376730021207No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2440.2452433839566603No Hit
CCTAGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2390.2402179047772205No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCA2280.22916185058245303No Hit
CCTAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2170.21810579638768557No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC2110.21207522137235785No Hit
CCTAGAGGGGGTACTTGAGAGATGGCTCAGAGGTTAAGACCACTGACTGCTCTTCC2100.2110701255364699No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCACTTCCG2050.20604464635703015No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2020.2030293588493663No Hit
CCTAGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1980.19900897550581448No Hit
CCTAGAGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAA1950.19599368799815062No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAA1950.19599368799815062No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.18493763380338316No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCC1840.18493763380338316No Hit
CCTAGAGGGGGTGCAGCGGGAGGTTTTTAGGAGGGCTGTTTTGCCAAGGGTCAATC1820.18292744213160725No Hit
CCTAGAGGGGTTCAGATGTGACTGGAATCCCTTTCTGCTAATGTTCATGTGCCTGT1810.1819223462957193No Hit
CCTAGAGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCACTGCTCTTCCG1760.17689686711627953No Hit
CCTAGAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA1760.17689686711627953No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA1710.17187138793683976No Hit
CCTAGAGGGAGGCTATTTGAATTCATTTAAATGAAATACTGAAGAAATTTGTTCCT1680.16885610042917593No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1650.16584081292151207No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC1630.16383062124973616No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA1580.15880514207029642No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC1550.15578985456263253No Hit
CCTAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA1500.1507643753831928No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC1480.14875418371141688No Hit
CCTAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1450.14573889620375305No Hit
CCTAGAGGGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGAC1430.14372870453197711No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA1410.1417185128602012No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTC1390.1397083211884253No Hit
CCTAGAGGGGGGACTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC1380.13870322535253737No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1370.13769812951664942No Hit
CCTAGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC1370.13769812951664942No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1300.13066245866543374No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCC1280.12865226699365784No Hit
CCTAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1260.12664207532188193No Hit
CCTAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.12563697948599398No Hit
CCTAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC1250.12563697948599398No Hit
CCTAGAGGGGAATTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAA1240.12463188365010604No Hit
CCTAGAGGGGCCTTTTCCTCTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1190.11960640447066628No Hit
CCTAGAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1190.11960640447066628No Hit
CCTAGAGGGACAGTAATAAAATGCATGTGTGACGTTAAGACTAGTGTCTGTCATAT1180.11860130863477832No Hit
CCTAGAGGGGAAGTAGAAACAGAGAAGGAAAGAATTTTGTGGTTGCTTGCTGAATT1160.11659111696300242No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC1140.11458092529122652No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCA1140.11458092529122652No Hit
CCTAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC1130.11357582945533858No Hit
CCTAGAGGGTGCTCCTAGAGGGGCTCTCCTTGCCCTCTCGTCTCCTAGGGCCGGGG1130.11357582945533858No Hit
CCTAGAGGGCAGCACCACATGCTCCATGTCAAGTGTGTACGTGGAGACCACTGGCT1120.11257073361945061No Hit
CCTAGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1070.10754525444001085No Hit
CCTAGAGGGGGGGCTGAAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC1070.10754525444001085No Hit
CCTAGAGGGGGGAATTCCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1060.10654015860412291No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA1050.10553506276823495No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA1030.10352487109645905No Hit
CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10352487109645905No Hit
CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1030.10352487109645905No Hit
CCTAGAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG1020.10251977526057109No Hit
CCTAGAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT1020.10251977526057109No Hit
CCTAGAGGGGATTTATTTTTTCTGGAGTGACAAAAGATTTAGGAAGGCTCCGCATG1020.10251977526057109No Hit
CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTG1010.10151467942468315No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTT1010.10151467942468315No Hit
CCTAGAGGGGGGAATTGGCCTGGAATTACATCTGGAGTGGGAAAGGAGTTGTAGGG1000.10050958358879519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTGC204.0220495E-450.3555150
GGATGAC150.00817607550.3555150
ATAAGTT150.00817607550.3555150
GAAACAC150.00817607550.3555150
TGATACA505.456968E-1250.3555150
TGCCTGT309.885534E-750.3555150
CCCCTAT251.9883646E-550.3555150
TCTCCCG204.0220495E-450.3555150
TACTTTA204.0220495E-450.3555150
TACTCAA204.0220495E-450.3555150
CAAGCCC150.00817607550.3555150
ATGAGAG204.0220495E-450.3555150
ACAGCGC505.456968E-1250.32995246
TCTCGCA251.9943529E-550.32995249
AATGGCC251.9943529E-550.32995249
GTGCCTG251.9943529E-550.32995249
TTCATTC150.00819251250.3299547
GAGGTAT309.920259E-750.3299547
GGCACAT150.00819251250.3299548
GAAATTC150.00819251250.3299548
Produced by FastQC (version 0.11.5)