##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212713_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99493 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.001296573628295 39.0 38.0 40.0 35.0 40.0 2 37.94892102962017 39.0 38.0 40.0 35.0 40.0 3 37.60128853286161 39.0 38.0 40.0 33.0 40.0 4 37.55531544932809 39.0 38.0 40.0 33.0 40.0 5 37.563999477350166 39.0 38.0 40.0 33.0 40.0 6 36.84579819685807 39.0 36.0 40.0 31.0 40.0 7 38.09893158312645 39.0 38.0 40.0 35.0 40.0 8 38.04462625511343 39.0 38.0 40.0 35.0 40.0 9 38.01586041229031 39.0 38.0 40.0 35.0 40.0 10 36.75011307328154 39.0 35.0 40.0 31.0 40.0 11 36.665343290482745 39.0 35.0 40.0 31.0 40.0 12 36.91615490537023 39.0 35.0 40.0 31.0 40.0 13 36.94146321851789 39.0 35.0 40.0 32.0 40.0 14 37.034866774546956 39.0 36.0 40.0 32.0 40.0 15 37.154855115435254 39.0 36.0 40.0 33.0 40.0 16 37.07678932186184 39.0 36.0 40.0 33.0 40.0 17 36.66642879398551 38.0 36.0 40.0 31.0 40.0 18 36.46156010975646 38.0 35.0 40.0 31.0 40.0 19 36.40918456574835 38.0 35.0 40.0 31.0 40.0 20 36.15288512759692 38.0 35.0 40.0 30.0 40.0 21 36.13211984762747 38.0 35.0 40.0 30.0 40.0 22 35.95007688983144 38.0 35.0 40.0 30.0 40.0 23 35.75309820791412 38.0 35.0 40.0 29.0 40.0 24 35.388198164695005 38.0 34.0 39.0 29.0 40.0 25 35.48231533876755 38.0 35.0 39.0 29.0 40.0 26 34.99231101685546 38.0 34.0 39.0 28.0 40.0 27 34.775129908636785 38.0 33.0 39.0 27.0 40.0 28 34.327369764707065 38.0 33.0 39.0 26.0 40.0 29 34.32160051460907 38.0 33.0 39.0 26.0 40.0 30 33.99972862412431 37.0 33.0 39.0 25.0 40.0 31 32.910988712773765 36.0 32.0 38.0 22.0 40.0 32 33.04295779602585 36.0 32.0 38.0 23.0 40.0 33 32.80349371312555 36.0 32.0 38.0 22.0 40.0 34 32.314424130340825 36.0 31.0 38.0 19.0 40.0 35 31.957986994059883 35.0 30.0 38.0 18.0 40.0 36 32.37504146020323 36.0 32.0 39.0 18.0 40.0 37 32.01469450112068 36.0 31.0 39.0 16.0 40.0 38 31.746163046646497 36.0 31.0 39.0 14.0 40.0 39 31.422703104741036 35.0 30.0 39.0 10.0 40.0 40 31.48234549164263 36.0 30.0 38.0 8.0 40.0 41 30.91022483993849 35.0 30.0 39.0 2.0 40.0 42 30.24912305388319 35.0 28.0 39.0 2.0 40.0 43 30.52323279024655 35.0 29.0 39.0 2.0 40.0 44 30.2429115616174 35.0 29.0 39.0 2.0 40.0 45 29.699094408651867 35.0 28.0 38.0 2.0 40.0 46 28.58440292281869 34.0 25.0 38.0 2.0 40.0 47 28.471359794156374 34.0 26.0 38.0 2.0 40.0 48 28.608253847004313 34.0 27.0 38.0 2.0 40.0 49 28.140864181399696 34.0 26.0 38.0 2.0 39.0 50 27.857788990180214 33.0 25.0 38.0 2.0 39.0 51 26.970771813092377 33.0 22.0 38.0 2.0 39.0 52 26.845496668107305 33.0 22.0 37.0 2.0 39.0 53 26.471701526740574 33.0 18.0 37.0 2.0 39.0 54 25.96905309921301 33.0 8.0 37.0 2.0 39.0 55 25.516337832812358 33.0 2.0 37.0 2.0 39.0 56 14.888102680590594 19.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.0800224431223668 1 2 -1.260575877937761 1 3 -1.9315573676120295 1 4 -0.6095407147474745 1 5 -1.8916790835172534 1 6 -0.9931574543214268 1 7 -1.6593190031569165 1 8 -1.4646783056954789 1 9 -1.7113607574486949 1 10 -3.199326564822222 1 11 -1.3143541409120232 1 12 -1.8507429867653329 1 13 -1.2313733679975698 1 14 -1.4451233123665332 1 15 -1.780466649505307 1 16 -2.0272857388320276 1 17 -1.736366140310409 1 18 -2.11672470381324 1 19 -2.592277229247678 1 20 -2.134691305351346 1 21 -2.0442239719026816 1 22 -1.5799577507334632 1 23 -2.1937962316059014 1 24 -2.218891031776643 1 25 -2.241304917690897 1 26 -2.8245806365775508 1 27 -2.34126513827637 1 28 -2.2622402795513423 1 29 -2.2451573802169555 1 30 -3.0761330212403664 1 31 -3.055460632224488 1 32 -2.5848978346899365 1 33 -2.239792275206021 1 34 -2.401465941406016 1 35 -2.0757525023870116 1 36 -1.898312749322855 1 37 -1.8871399695703488 1 38 -2.4340709512988496 1 39 -2.250730869628754 1 40 -2.320311220716139 1 41 -2.9601093815904704 1 42 -2.0806722196017375 1 43 -1.4586812797080313 1 44 -2.3290419646800125 1 45 -3.0279117081728693 1 46 -2.483174734753291 1 47 -2.3070323081749677 1 48 -2.0537403464552213 1 49 -2.4417546558386647 1 50 -2.5535798549032656 1 51 -2.466903854934163 1 52 -1.754967184025901 1 53 -2.440141251346745 1 54 -3.083646754856318 1 55 -3.096758654302345 1 56 -2.5920740651489886 2 1 -0.7339360970360218 2 2 -0.6717727891346712 2 3 -1.8352077712624322 2 4 -1.3215449267516846 2 5 -0.7879584797966501 2 6 -1.734294695458665 2 7 -0.502597346435266 2 8 -0.6259629669801399 2 9 -0.40756995365789095 2 10 -0.9158937813894426 2 11 -2.012669339227223 2 12 -1.6429507789731232 2 13 -1.673809310433512 2 14 -1.0702549374981558 2 15 -2.221674090712746 2 16 -2.7810589926052813 2 17 -1.3950182989625688 2 18 -2.388047975136516 2 19 -2.2711119080823607 2 20 -1.977794148454194 2 21 -2.085817513496224 2 22 -2.3753245461002592 2 23 -2.2673308051404746 2 24 -1.6278064406920478 2 25 -2.287636964022937 2 26 -1.448483760480677 2 27 -1.2041995012107307 2 28 -2.21029222760329 2 29 -1.2706049056644844 2 30 -2.2012646463719925 2 31 -1.8797849565488107 2 32 -1.2272284770205815 2 33 -2.598514633928378 2 34 -2.0585385984786733 2 35 -1.6262965529310627 2 36 -1.3317981828082885 2 37 -1.2383158207461982 2 38 -1.4591674763953755 2 39 -0.4627350816329674 2 40 -0.4554463558512758 2 41 0.3921194706383808 2 42 -0.6059442448737649 2 43 -0.6004854215121718 2 44 -0.4167920524301003 2 45 -1.0131696934308536 2 46 -0.9263126778912323 2 47 -1.7408687420114042 2 48 -1.307513600228475 2 49 -0.7673074813914909 2 50 -1.6955594968829075 2 51 -2.320185708216016 2 52 -1.6033350323937512 2 53 -2.4827877939932854 2 54 -1.0155526867622484 2 55 -1.3666779242216123 2 56 -1.7616072346821579 3 1 -0.2728971359970558 3 2 0.11718824982636278 3 3 -0.11767530372996049 3 4 -0.5244670046737596 3 5 0.3841194422812748 3 6 -0.38689209805606595 3 7 -0.06103890487682406 3 8 -0.2444694604866342 3 9 -0.24360891469684987 3 10 -0.5344002748959369 3 11 -0.9006563522142343 3 12 -0.37347025949260626 3 13 -0.34101710264130247 3 14 0.16513467289145467 3 15 -1.3320637011023564 3 16 -0.8135265250728168 3 17 -0.5849533638976325 3 18 -1.1753856374741716 3 19 -0.07630671327716243 3 20 -0.8008460965061417 3 21 -0.3845188121975198 3 22 -0.5133115590872706 3 23 -0.7364866492963245 3 24 -0.21059864848425747 3 25 -0.6236759250619031 3 26 -0.32673051372743345 3 27 -0.10355015056138228 3 28 -0.012240279551342326 3 29 -0.4589165939761699 3 30 -0.07951139961874532 3 31 0.24521504345118927 3 32 0.9058884060963024 3 33 0.5378490024352587 3 34 1.1427601028200272 3 35 0.34285929122478365 3 36 1.3808641548540521 3 37 0.7925283350979591 3 38 1.2778455106176132 3 39 1.0161610222631374 3 40 0.39682637142145083 3 41 1.5690675225864368 3 42 1.3843154953859766 3 43 1.2274366564099068 3 44 0.18385729821925167 3 45 0.5777393974782363 3 46 1.1694665428879851 3 47 1.1714689203262587 3 48 0.9213825036676297 3 49 1.2099652458812358 3 50 0.9083366070131973 3 51 0.9882558502255421 3 52 0.8301065260478069 3 53 1.0561732449677521 3 54 -0.1275656737752371 3 55 0.2518285692848785 3 56 0.9429382198632972 4 1 -0.7878568598175448 4 2 -0.5108324636373851 4 3 -1.3826350275504495 4 4 -0.8758766709568491 4 5 -0.7105295795829392 4 6 -1.188675757434666 4 7 -0.5777247506765946 4 8 -0.9756259622127601 4 9 -1.0912786500248117 4 10 -1.5912184567141168 4 11 -0.7356678596940966 4 12 -1.179856910815964 4 13 -1.0218283799715664 4 14 -0.6462865008714971 4 15 -1.4085884421840618 4 16 -0.9775081131050385 4 17 -1.4772145836904969 4 18 -0.8317435200978807 4 19 -0.9059960113208945 4 20 -0.6772845314888798 4 21 -0.3143231850398678 4 22 -0.5091400976373279 4 23 -0.7858249692042634 4 24 -1.2418126876096878 4 25 -1.4178358790319834 4 26 -0.9267017450277777 4 27 -1.1734580907224839 4 28 -1.2622402795513423 4 29 -0.9025299426528122 4 30 -0.6760303869605195 4 31 -0.8544685008526116 4 32 -1.1220172325688296 4 33 -0.5271682587845348 4 34 -1.3169637176632847 4 35 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0.35684836693673816 5 20 1.0805710157933248 5 21 0.2293849311179912 5 22 1.009549403479575 5 23 0.181561479045925 5 24 0.713064453120019 5 25 0.40480001076697647 5 26 0.9266117322618754 5 27 1.3571450366043933 5 28 0.5612891322133606 5 29 1.1683561332965553 5 30 0.9984297768518431 5 31 1.461391514039427 5 32 1.3521450905882801 5 33 1.0314318901357922 5 34 1.451316252552651 5 35 1.3975384356098104 5 36 1.5363454382765056 5 37 1.0405229875043709 5 38 0.2904123555374021 5 39 1.0293963163807867 5 40 0.744553644148727 5 41 0.2921958648324221 5 42 1.0883261905731416 5 43 0.7918751590837019 5 44 1.304980292871658 5 45 0.785760787852567 5 46 2.535108254117933 5 47 1.6401854978663657 5 48 2.4445108459136193 5 49 1.4808208608545002 5 50 1.3238446284035703 5 51 1.4831756363217998 5 52 0.9957482372777555 5 53 0.8887935658233665 5 54 1.3033166791659383 5 55 1.4380585158089438 5 56 1.4801039952643649 6 1 -0.35130622690614643 6 2 -0.7771299319918157 6 3 -0.8210843946390511 6 4 -1.0301488228555797 6 5 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0.32784154155772427 7 31 0.5908032787453052 7 32 0.434498031764754 7 33 0.43143189013579075 7 34 0.7924927231408887 7 35 0.04459725913921986 7 36 1.2422277912176867 7 37 1.2052288698573186 7 38 1.4315888261256369 7 39 0.7470433752043171 7 40 1.8504359970898996 7 41 2.0921958648324264 7 42 1.2177379552790235 7 43 2.074228100260175 7 44 2.093215586989306 7 45 1.7387019643231554 7 46 2.0409906070591113 7 47 2.3107737331604845 7 48 2.0445108459136208 7 49 2.3631738020309676 7 50 2.4297269813447464 7 51 3.824352106910034 7 52 3.4663364725718715 7 53 3.7711465069998376 7 54 4.385669620342409 7 55 3.3439408687501206 7 56 2.1742216423231895 8 1 -0.546733056174439 8 2 -0.5829226149186439 8 3 -0.7024868336634427 8 4 -0.04691711553850553 8 5 -0.3758583847697281 8 6 -0.6823466435106127 8 7 -0.5189103017726069 8 8 -0.5839927420830904 8 9 -0.8652829723465203 8 10 -1.347316017689728 8 11 -2.0589157757175585 8 12 -2.606840547740944 8 13 -1.59129426450383 8 14 -1.9820382761107638 8 15 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-2.0781047583659173 120 46 -1.7522867038652592 120 47 -0.9707388718815331 120 48 -2.4386824313973037 120 49 -1.688086702170711 120 50 -2.4501049514283615 120 51 -2.6058999939303042 120 52 -1.8487895778482937 120 53 -2.2775929887984816 120 54 -2.0311371023466656 120 55 -0.7199246774683665 120 56 1.1137174406425174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66.0 3 12.0 4 30.0 5 24.0 6 55.0 7 107.0 8 62.0 9 127.0 10 176.0 11 194.0 12 303.0 13 382.0 14 474.0 15 548.0 16 671.0 17 674.0 18 787.0 19 844.0 20 1027.0 21 1066.0 22 1103.0 23 1332.0 24 1614.0 25 1942.0 26 1927.0 27 2006.0 28 2254.0 29 2557.0 30 2994.0 31 3567.0 32 4305.0 33 5533.0 34 6970.0 35 9277.0 36 11805.0 37 14012.0 38 12547.0 39 6119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 70.34062697878242 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17.045420280823777 23.962489823404663 17.757028132632446 24 36.1894806669816 21.300996049973364 23.84589870644166 18.66362457660338 25 31.344829319792872 23.12000402191946 19.248906540646523 26.28626011764115 26 28.470133867058244 15.191044686051072 25.792792702183508 30.546028744707172 27 20.38440251572327 19.187924528301885 25.738867924528304 34.68880503144654 28 26.976922070471726 26.012741674131178 22.19023943477692 24.820096820620176 29 32.19937983247423 18.817453286082475 21.042404961340207 27.940761920103093 30 26.78726104385311 18.38728521644542 28.268839514130896 26.556614225570573 31 36.74100487529715 15.180909786856844 27.424553769289655 20.65353156855635 32 42.71490476670362 11.422956767106722 26.52222110248917 19.339917363700494 33 43.86688308415078 12.378139145688621 26.622375013862143 17.132602756298454 34 29.97691136585907 14.765635239910067 39.12767308913826 16.129780305092606 35 22.446654027671332 27.90528820943084 37.7516034044613 11.89645435843653 36 29.773002421307503 30.42776432606941 25.849475383373687 13.949757869249394 37 30.336761158934717 26.72795713031456 26.85914967050489 16.076132040245835 38 31.1585790573485 23.986230706332464 25.860833223973106 18.994357012345926 39 30.608412998051705 24.458666882022186 28.12004724361757 16.81287287630854 40 32.94040404040404 18.443434343434344 25.4 23.216161616161614 41 29.636176316108443 19.022463738838834 29.538200476748415 21.80315946830431 42 21.675613923346777 23.70669173920516 30.176013264181655 24.441681073266405 43 28.950800117262922 14.07256148721733 27.105930875529456 29.870707519990297 44 24.436694465504168 12.11928228455901 30.215820065706346 33.22820318423047 45 29.482265127992473 18.343733880837032 26.991190719407726 25.18281027176277 46 25.390482741876745 20.128070246429004 30.660988143891878 23.820458867802373 47 25.122195573636112 17.687240049789004 32.41446309845472 24.776101278120162 48 28.335780466811805 14.712166472583665 31.81206014885322 25.139992911751303 49 26.212108948156544 14.762839451212109 32.74920069610264 26.275850904528713 50 20.378038532797422 14.264019519504318 33.79972259625606 31.558219351442197 51 17.81262972450312 15.361406137677564 39.97995281824901 26.8460113195703 52 18.317719147212237 15.675292449485998 41.33995037220844 24.66703803109333 53 15.926334876465045 16.995046445900908 41.42245003393539 25.656168643698653 54 18.341779849989866 17.289681735252383 40.037502533955 24.331035880802755 55 17.18327270516471 15.567084477500027 35.185279007792154 32.06436380954311 56 16.441229216693518 20.733152983373355 27.171038633393113 35.65457916654002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 4.0 11 8.0 12 48.5 13 91.5 14 94.0 15 123.5 16 153.0 17 340.0 18 527.0 19 689.5 20 852.0 21 841.0 22 886.5 23 943.0 24 1085.5 25 1228.0 26 1476.5 27 1725.0 28 2009.0 29 2251.5 30 2210.0 31 2248.0 32 2286.0 33 1757.0 34 1228.0 35 1570.5 36 1913.0 37 1354.0 38 812.5 39 830.0 40 786.5 41 743.0 42 994.0 43 1245.0 44 1188.5 45 1132.0 46 1380.5 47 1617.0 48 1605.0 49 1483.0 50 1361.0 51 1608.5 52 1856.0 53 2934.0 54 4219.5 55 4427.0 56 4821.5 57 5216.0 58 6848.0 59 8480.0 60 8594.5 61 8709.0 62 9910.5 63 11500.5 64 11889.0 65 9393.0 66 6897.0 67 5578.5 68 4260.0 69 3369.0 70 2478.0 71 1944.5 72 1490.5 73 1570.0 74 1129.5 75 689.0 76 513.5 77 338.0 78 272.5 79 157.5 80 108.0 81 87.0 82 66.0 83 44.0 84 22.0 85 17.0 86 12.0 87 12.5 88 7.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.038193641763742174 26 0.06633632516860483 27 0.11860130863477832 28 0.13468284200898556 29 0.16584081292151207 30 0.20805483802880603 31 0.21810579638768557 32 0.2643402048385314 33 0.3035389424381615 34 0.31157970912526506 35 0.3306765300071362 36 0.37490074678620605 37 0.40404852602695673 38 0.43420140110359523 39 0.43420140110359523 40 0.49551224709276026 41 0.4914918637492085 42 0.5829555848150122 43 0.5729046264561326 44 0.5708944347843568 45 0.6231594182505301 46 0.6442664308041771 47 0.6794447850602555 48 0.7417607268853086 49 0.6603479641783844 50 0.7236690018393254 51 0.729699576854653 52 0.7618626436030675 53 0.7799543686490507 54 0.837244831294664 55 0.8080970520539135 56 0.8000562853668097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 99493.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.28433156101434 #Duplication Level Percentage of deduplicated Percentage of total 1 71.13018492595594 19.407395495160465 2 10.852427613644736 5.922024665051813 3 4.86259485743756 3.98017951011629 4 2.7444190672658957 2.9951855909460967 5 1.9560892949237456 2.668529444282512 6 1.4366757533338244 2.3519242559778073 7 1.1235541147867087 2.1458796096207773 8 0.7920135563250571 1.728764837727277 9 0.6483459809916746 1.5920718040465158 >10 3.702202902821779 18.80232780195592 >50 0.4125838060856112 7.9663895952479065 >100 0.2947027186325794 16.078518086699567 >500 0.018418919914536213 3.4635602504698824 >1k 0.0257864878803507 10.897249052697175 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 2673 2.6866211693284954 No Hit CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 2124 2.13482355542601 No Hit CCTAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 1346 1.3528589951051833 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1307 1.3136602575055532 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1243 1.2493341240087241 No Hit CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1091 1.0965595569537556 No Hit CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1058 1.063391394369453 No Hit CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 884 0.8885047189249494 No Hit CCTAGAGGGGTGCGATGGCTTAGCAGGTCAAGGCACTCGCTACCAAGCTGTCGTGT 722 0.7256791935111013 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 666 0.669393826701376 No Hit CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 589 0.5920014473380036 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCA 585 0.5879810639944518 No Hit CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 498 0.5005377262722001 No Hit CCTAGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC 495 0.4975224387645362 No Hit CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 438 0.44023197611892295 No Hit CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTA 427 0.42917592192415543 No Hit CCTAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 412 0.4140994843858362 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 357 0.3588192134119988 No Hit CCTAGAGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 355 0.35680902174022294 No Hit CCTAGAGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC 353 0.35479883006844704 No Hit CCTAGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 345 0.3467580633813434 No Hit CCTAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 342 0.3437427758736795 No Hit CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 338 0.33972239253012776 No Hit CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT 334 0.33570200918657594 No Hit CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 327 0.3286663383353603 No Hit CCTAGAGGGGAGCCAAGGCCAAGGAATTCACCAATGTTTATATCAAAAACTTTGGA 310 0.31157970912526506 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 302 0.3035389424381615 No Hit CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 294 0.29549817575105786 No Hit CCTAGAGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC 286 0.28745740906395423 No Hit CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGC 285 0.2864523132280663 No Hit CCTAGAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 267 0.26836058818208314 No Hit CCTAGAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 261 0.2623300131667554 No Hit CCTAGAGGGGAGCAATGCGCGTGGTACGCGGCAGTGACACCCGCCACAGCGCAGCT 248 0.24926376730021207 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 244 0.2452433839566603 No Hit CCTAGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 239 0.2402179047772205 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCA 228 0.22916185058245303 No Hit CCTAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 217 0.21810579638768557 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 211 0.21207522137235785 No Hit CCTAGAGGGGGTACTTGAGAGATGGCTCAGAGGTTAAGACCACTGACTGCTCTTCC 210 0.2110701255364699 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCACTTCCG 205 0.20604464635703015 No Hit CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 202 0.2030293588493663 No Hit CCTAGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 198 0.19900897550581448 No Hit CCTAGAGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAA 195 0.19599368799815062 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAA 195 0.19599368799815062 No Hit CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.18493763380338316 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCC 184 0.18493763380338316 No Hit CCTAGAGGGGGTGCAGCGGGAGGTTTTTAGGAGGGCTGTTTTGCCAAGGGTCAATC 182 0.18292744213160725 No Hit CCTAGAGGGGTTCAGATGTGACTGGAATCCCTTTCTGCTAATGTTCATGTGCCTGT 181 0.1819223462957193 No Hit CCTAGAGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCACTGCTCTTCCG 176 0.17689686711627953 No Hit CCTAGAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA 176 0.17689686711627953 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA 171 0.17187138793683976 No Hit CCTAGAGGGAGGCTATTTGAATTCATTTAAATGAAATACTGAAGAAATTTGTTCCT 168 0.16885610042917593 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 165 0.16584081292151207 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC 163 0.16383062124973616 No Hit CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA 158 0.15880514207029642 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC 155 0.15578985456263253 No Hit CCTAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA 150 0.1507643753831928 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC 148 0.14875418371141688 No Hit CCTAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 145 0.14573889620375305 No Hit CCTAGAGGGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGAC 143 0.14372870453197711 No Hit CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA 141 0.1417185128602012 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTC 139 0.1397083211884253 No Hit CCTAGAGGGGGGACTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC 138 0.13870322535253737 No Hit CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 137 0.13769812951664942 No Hit CCTAGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC 137 0.13769812951664942 No Hit CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 130 0.13066245866543374 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCC 128 0.12865226699365784 No Hit CCTAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 126 0.12664207532188193 No Hit CCTAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.12563697948599398 No Hit CCTAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC 125 0.12563697948599398 No Hit CCTAGAGGGGAATTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAA 124 0.12463188365010604 No Hit CCTAGAGGGGCCTTTTCCTCTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 119 0.11960640447066628 No Hit CCTAGAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 119 0.11960640447066628 No Hit CCTAGAGGGACAGTAATAAAATGCATGTGTGACGTTAAGACTAGTGTCTGTCATAT 118 0.11860130863477832 No Hit CCTAGAGGGGAAGTAGAAACAGAGAAGGAAAGAATTTTGTGGTTGCTTGCTGAATT 116 0.11659111696300242 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC 114 0.11458092529122652 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCA 114 0.11458092529122652 No Hit CCTAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC 113 0.11357582945533858 No Hit CCTAGAGGGTGCTCCTAGAGGGGCTCTCCTTGCCCTCTCGTCTCCTAGGGCCGGGG 113 0.11357582945533858 No Hit CCTAGAGGGCAGCACCACATGCTCCATGTCAAGTGTGTACGTGGAGACCACTGGCT 112 0.11257073361945061 No Hit CCTAGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 107 0.10754525444001085 No Hit CCTAGAGGGGGGGCTGAAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC 107 0.10754525444001085 No Hit CCTAGAGGGGGGAATTCCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 106 0.10654015860412291 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA 105 0.10553506276823495 No Hit CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA 103 0.10352487109645905 No Hit CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.10352487109645905 No Hit CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 103 0.10352487109645905 No Hit CCTAGAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG 102 0.10251977526057109 No Hit CCTAGAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT 102 0.10251977526057109 No Hit CCTAGAGGGGATTTATTTTTTCTGGAGTGACAAAAGATTTAGGAAGGCTCCGCATG 102 0.10251977526057109 No Hit CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTG 101 0.10151467942468315 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTT 101 0.10151467942468315 No Hit CCTAGAGGGGGGAATTGGCCTGGAATTACATCTGGAGTGGGAAAGGAGTTGTAGGG 100 0.10050958358879519 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0010050958358879519 0.0 0.0 0.0 0.0 12 0.0010050958358879519 0.0 0.0 0.0 0.0 13 0.0010050958358879519 0.0 0.0 0.0 0.0 14 0.004020383343551807 0.0 0.0 0.0 0.0 15 0.004020383343551807 0.0 0.0 0.0 0.0 16 0.004020383343551807 0.0 0.0 0.0 0.0 17 0.004020383343551807 0.0 0.0 0.0 0.0 18 0.004020383343551807 0.0 0.0 0.0 0.0 19 0.005025479179439759 0.0 0.0 0.0 0.0 20 0.007035670851215663 0.0 0.0 0.0 0.0 21 0.007035670851215663 0.0 0.0 0.0 0.0 22 0.008040766687103615 0.0 0.0 0.0 0.0 23 0.008040766687103615 0.0 0.0 0.0 0.0 24 0.01105605419476747 0.0 0.0 0.0 0.0 25 0.01105605419476747 0.0 0.0 0.0 0.0 26 0.01105605419476747 0.0 0.0 0.0 0.0 27 0.01105605419476747 0.0 0.0 0.0 0.0 28 0.019096820881871087 0.0 0.0 0.0 0.0 29 0.02110701255364699 0.0 0.0 0.0 0.0 30 0.024122300061310845 0.0 0.0 0.0 0.0 31 0.025127395897198798 0.0 0.0 0.0 0.0 32 0.02613249173308675 0.0 0.0 0.0 0.0 33 0.02613249173308675 0.0 0.0 0.0 0.0 34 0.027137587568974702 0.0 0.0 0.0 0.0 35 0.03417325842019037 0.0 0.0 0.0 0.0 36 0.03517835425607832 0.0 0.0 0.0 0.0 37 0.03618345009196627 0.0 0.0 0.0 0.0 38 0.038193641763742174 0.0 0.0 0.0 0.0 39 0.038193641763742174 0.0 0.0 0.0 0.0 40 0.04120892927140603 0.0 0.0 0.0 0.0 41 0.04221402510729398 0.0 0.0 0.0 0.0 42 0.04422421677906988 0.0 0.0 0.0 0.0 43 0.04422421677906988 0.0 0.0 0.0 0.0 44 0.045229312614957835 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCTGC 20 4.0220495E-4 50.35551 50 GGATGAC 15 0.008176075 50.35551 50 ATAAGTT 15 0.008176075 50.35551 50 GAAACAC 15 0.008176075 50.35551 50 TGATACA 50 5.456968E-12 50.35551 50 TGCCTGT 30 9.885534E-7 50.35551 50 CCCCTAT 25 1.9883646E-5 50.35551 50 TCTCCCG 20 4.0220495E-4 50.35551 50 TACTTTA 20 4.0220495E-4 50.35551 50 TACTCAA 20 4.0220495E-4 50.35551 50 CAAGCCC 15 0.008176075 50.35551 50 ATGAGAG 20 4.0220495E-4 50.35551 50 ACAGCGC 50 5.456968E-12 50.329952 46 TCTCGCA 25 1.9943529E-5 50.329952 49 AATGGCC 25 1.9943529E-5 50.329952 49 GTGCCTG 25 1.9943529E-5 50.329952 49 TTCATTC 15 0.008192512 50.32995 47 GAGGTAT 30 9.920259E-7 50.32995 47 GGCACAT 15 0.008192512 50.32995 48 GAAATTC 15 0.008192512 50.32995 48 >>END_MODULE