FastQCFastQC Report
Tue 31 May 2016
SRR212712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94776
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC26462.791846036971385No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC21052.221026420190766No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC16951.7884274499873385No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12101.27669452181987No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11321.1943952055372669No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC11171.1785684139444585No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9440.9960327509074027No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9320.9833713176331561No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6830.7206465771925382No Hit
CCTAGAGGGGTGCGATGGCTTAGCAGGTCAAGGCACTCGCTACCAAGCTGTCGTGT6650.7016544272811682No Hit
CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA5830.6151346332404828No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5810.6130243943614417No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCA5770.6088039166033595No Hit
CCTAGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC4770.5032919726513041No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTA4240.44737064235671475No Hit
CCTAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4180.44103992571959144No Hit
CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3820.4030556258968515No Hit
CCTAGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC3660.3861737148645227No Hit
CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3630.383008356545961No Hit
CCTAGAGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3590.3787878787878788No Hit
CCTAGAGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC3520.3714020427112349No Hit
CCTAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3220.3397484595256183No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC3200.33763822064657717No Hit
CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGC3130.3302523845699333No Hit
CCTAGAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3050.32181142905376886No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT2950.31126023465856334No Hit
CCTAGAGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC2850.3007090402633578No Hit
CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2770.2922680847471934No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.2912129653076728No Hit
CCTAGAGGGGAGCAATGCGCGTGGTACGCGGCAGTGACACCCGCCACAGCGCAGCT2750.29015784586815224No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC2740.2891027264286317No Hit
CCTAGAGGGGAGCCAAGGCCAAGGAATTCACCAATGTTTATATCAAAAACTTTGGA2510.264834979319659No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2360.24900818772685068No Hit
CCTAGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2310.24373259052924792No Hit
CCTAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2130.22474044061787796No Hit
CCTAGAGGGGTTCAGATGTGACTGGAATCCCTTTCTGCTAATGTTCATGTGCCTGT2130.22474044061787796No Hit
CCTAGAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC2090.22051996285979572No Hit
CCTAGAGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAA2080.2194648434202752No Hit
CCTAGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2080.2194648434202752No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCA1950.205748290706508No Hit
CCTAGAGGGACAGTAATAAAATGCATGTGTGACGTTAAGACTAGTGTCTGTCATAT1910.20152781294842576No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCC1900.20047269350890523No Hit
CCTAGAGGGGGTACTTGAGAGATGGCTCAGAGGTTAAGACCACTGACTGCTCTTCC1840.1941419768717819No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA1790.1888663796741791No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.18675614079513803No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC1770.18675614079513803No Hit
CCTAGAGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCACTGCTCTTCCG1760.18570102135561745No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1730.18253566303705582No Hit
CCTAGAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA1730.18253566303705582No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCACTTCCG1700.17937030471849413No Hit
CCTAGAGGGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGAC1650.17409470752089137No Hit
CCTAGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC1630.17198446864185027No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAA1590.16776399088376803No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC1560.16459863256520638No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC1540.16248839368616527No Hit
CCTAGAGGGGGTGCAGCGGGAGGTTTTTAGGAGGGCTGTTTTGCCAAGGGTCAATC1540.16248839368616527No Hit
CCTAGAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1510.15932303536760362No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1500.15826791592808306No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1490.1572127964885625No Hit
CCTAGAGGGGAATTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAA1390.146661602093357No Hit
CCTAGAGGGGGGACTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC1390.146661602093357No Hit
CCTAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1260.13294504937958976No Hit
CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1260.13294504937958976No Hit
CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA1250.1318899299400692No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCC1230.1297796910610281No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA1210.127669452181987No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC1200.12661433274246645No Hit
CCTAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.1255592133029459No Hit
CCTAGAGGGGGGAATTCCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1180.12450409386342534No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTC1170.12344897442390479No Hit
CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA1150.12133873554486367No Hit
CCTAGAGGGCAGCACCACATGCTCCATGTCAAGTGTGTACGTGGAGACCACTGGCT1150.12133873554486367No Hit
CCTAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC1130.11922849666582258No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC1100.1160631383472609No Hit
CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.11500801890774036No Hit
CCTAGAGGGGATTTATTTTTTCTGGAGTGACAAAAGATTTAGGAAGGCTCCGCATG1060.11184266058917869No Hit
CCTAGAGGGGGGATGCAGGCATGCTTCCCACTGTTCAGAGCTCTGGCTTCCATCTG1050.11078754114965815No Hit
CCTAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAA1040.1097324217101376No Hit
CCTAGAGGGAGGCTATTTGAATTCATTTAAATGAAATACTGAAGAAATTTGTTCCT1030.10867730227061703No Hit
CCTAGAGGGCAAATCTGGAACTGTCTTTCTCCCCAGCTCTGCCCCCTTCATTCCTA1020.10762218283109648No Hit
CCTAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA1010.10656706339157591No Hit
CCTAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1000.10551194395205538No Hit
CCTAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC1000.10551194395205538No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA1000.10551194395205538No Hit
CCTAGAGGGGCCTTTTCCTCTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1000.10551194395205538No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC990.10445682451253482No Hit
CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTG990.10445682451253482No Hit
CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC990.10445682451253482No Hit
CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC970.1023465856334937No Hit
CCTAGAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT960.10129146619397315No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTC960.10129146619397315No Hit
CCTAGAGGGAGCCATTCCTAGAATTCCACTGTCACCCATTCGAAGAACTGGTCCAG960.10129146619397315No Hit
CCTAGAGGGGAAGTAGAAACAGAGAAGGAAAGAATTTTGTGGTTGCTTGCTGAATT960.10129146619397315No Hit
CCTAGAGGGGGGAATTGGCCTGGAATTACATCTGGAGTGGGAAAGGAGTTGTAGGG960.10129146619397315No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTT960.10129146619397315No Hit
CCTAGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA950.10023634675445262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATG204.048118E-450.28716350
GAACAAG204.048118E-450.28716349
TGGTCTG252.0036801E-550.2871650
TTGCAAT150.008218897550.2871649
AGCTTGT150.008218897550.2871649
GTCATAT252.0036801E-550.2871650
AATTCTC150.008218897550.2871650
TTGTAGG150.008218897550.2871649
CCCGGTC150.008218897550.2871649
TGTCATA252.0036801E-550.2871649
GGATTAA252.0036801E-550.2871649
TTATATG252.0036801E-550.2871650
CTGAATT150.008218897550.2871650
TGTAGGG150.008218897550.2871650
TTGATCC150.008218897550.2871650
AGATGAA150.008218897550.2871650
GTAGGCT150.008218897550.2871649
GATTAAA252.0036801E-550.2871650
ACAACTG150.008218897550.2871650
TGTCGTG850.050.2871649
Produced by FastQC (version 0.11.5)