##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212712_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94776 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.307852198868915 39.0 38.0 40.0 35.0 40.0 2 38.25312315354098 39.0 38.0 40.0 35.0 40.0 3 37.97412847134296 39.0 38.0 40.0 35.0 40.0 4 37.924769983962186 39.0 38.0 40.0 35.0 40.0 5 37.91310036296109 39.0 38.0 40.0 35.0 40.0 6 37.30572085760108 39.0 37.0 40.0 33.0 40.0 7 38.359131003629614 39.0 38.0 40.0 36.0 40.0 8 38.304412509496075 39.0 38.0 40.0 36.0 40.0 9 38.30162699417574 39.0 38.0 40.0 35.0 40.0 10 37.217681691567485 39.0 36.0 40.0 32.0 40.0 11 37.127732759348355 39.0 36.0 40.0 32.0 40.0 12 37.349772094201064 39.0 37.0 40.0 33.0 40.0 13 37.41603359500296 39.0 37.0 40.0 33.0 40.0 14 37.458217270194986 39.0 37.0 40.0 33.0 40.0 15 37.566978982020764 39.0 38.0 40.0 33.0 40.0 16 37.48675825103402 39.0 38.0 40.0 33.0 40.0 17 37.145564277876254 39.0 36.0 40.0 33.0 40.0 18 36.944131425677384 39.0 36.0 40.0 32.0 40.0 19 36.917901156410906 39.0 36.0 40.0 32.0 40.0 20 36.66292099265637 38.0 36.0 40.0 31.0 40.0 21 36.50342913817844 38.0 36.0 40.0 31.0 40.0 22 36.37246771334515 38.0 36.0 40.0 31.0 40.0 23 36.16658225711151 38.0 35.0 40.0 30.0 40.0 24 35.80748290706508 38.0 35.0 40.0 29.0 40.0 25 35.92128808981177 38.0 35.0 40.0 30.0 40.0 26 35.44167299738331 38.0 35.0 39.0 29.0 40.0 27 35.17295517852621 38.0 35.0 39.0 28.0 40.0 28 34.79448383557018 38.0 34.0 39.0 27.0 40.0 29 34.76876002363468 38.0 34.0 39.0 27.0 40.0 30 34.44373048029037 38.0 33.0 39.0 27.0 40.0 31 33.37312188739765 36.0 32.0 39.0 23.0 40.0 32 33.47740989279986 37.0 33.0 39.0 23.0 40.0 33 33.25547606989111 36.0 32.0 38.0 23.0 40.0 34 32.87129653076728 36.0 31.0 39.0 22.0 40.0 35 32.49852283278467 36.0 31.0 39.0 20.0 40.0 36 32.79384021271208 36.0 32.0 39.0 20.0 40.0 37 32.38184772516249 36.0 32.0 39.0 18.0 40.0 38 32.15883768042542 36.0 32.0 39.0 16.0 40.0 39 31.76655482400608 36.0 31.0 39.0 13.0 40.0 40 31.88499198109226 36.0 31.0 39.0 10.0 40.0 41 31.21701696632059 36.0 30.0 39.0 2.0 40.0 42 30.458417742888496 35.0 29.0 39.0 2.0 40.0 43 30.833111758251032 35.0 30.0 39.0 2.0 40.0 44 30.547132185363385 35.0 29.0 39.0 2.0 40.0 45 29.945787963197432 35.0 29.0 38.0 2.0 40.0 46 28.84755634337807 34.0 26.0 38.0 2.0 40.0 47 28.777791846036973 34.0 27.0 38.0 2.0 40.0 48 28.95352198868912 34.0 28.0 38.0 2.0 40.0 49 28.491358571790325 34.0 26.0 38.0 2.0 40.0 50 28.183168734700768 34.0 26.0 38.0 2.0 39.0 51 27.243627078585295 33.0 23.0 38.0 2.0 39.0 52 27.139001434962438 33.0 23.0 37.0 2.0 39.0 53 26.7720097915084 33.0 22.0 37.0 2.0 39.0 54 26.281305393770573 33.0 15.0 37.0 2.0 39.0 55 25.723136659069805 33.0 2.0 37.0 2.0 39.0 56 15.044304465265467 19.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.4450377489951691 1 2 -0.8187562057548305 1 3 -1.0751654151676107 1 4 -1.2142494163227795 1 5 -0.8902396390093372 1 6 -0.9761067712096363 1 7 -0.5686028314962996 1 8 -0.3741546143265069 1 9 -0.5556430536471524 1 10 -1.0089637280032306 1 11 -0.8958843697259056 1 12 -1.4248270969635541 1 13 -1.4894741552954613 1 14 -1.4433155829369824 1 15 -1.0369953650587647 1 16 -0.5149076631909537 1 17 -0.4750691825705715 1 18 -0.022047987255810142 1 19 -0.3683439145913425 1 20 -0.7707226304070289 1 21 -0.19739293175734218 1 22 -0.22675116189985545 1 23 0.20374792522206064 1 24 -0.6976721169751983 1 25 -0.2819373571183874 1 26 0.06278444171773145 1 27 0.5742068853005193 1 28 0.6086124997856217 1 29 0.43064342238662334 1 30 0.3037345447976989 1 31 -0.36245769928993354 1 32 0.4808545462590388 1 33 -1.2172613757226607 1 34 -0.1357734787825322 1 35 0.7642282182074709 1 36 0.021194688394345462 1 37 0.6920743199880448 1 38 0.21360602264742923 1 39 0.1118486793829021 1 40 0.4085303022188498 1 41 0.9095126589495308 1 42 0.6822227915050227 1 43 0.4215578223402652 1 44 0.7665045145015341 1 45 0.8782346239297958 1 46 1.1410102529161108 1 47 0.698019757168538 1 48 -0.2734030633485922 1 49 -0.2794347864227156 1 50 0.7829174944131054 1 51 0.10465643566595872 1 52 1.0593262962096652 1 53 1.0001344873058038 1 54 1.0553672164838481 1 55 1.4894763461249312 1 56 0.33895697700293503 2 1 -0.7393062256165877 2 2 -0.45359783471410253 2 3 -0.5822544046095359 2 4 -0.8145510754479659 2 5 -0.8314161095975692 2 6 -1.2367402553725313 2 7 -0.34854249967126094 2 8 -0.5089962432857789 2 9 -0.3334711079457975 2 10 -1.2975006812460634 2 11 -2.1459597845072054 2 12 -1.6773157847463551 2 13 -1.5879658596695165 2 14 -1.4657891877635336 2 15 -1.9851099955263365 2 16 -0.7321022333266995 2 17 -1.1759741599461364 2 18 -0.3194838846917065 2 19 -0.9496410488296547 2 20 -0.5580529471491076 2 21 -0.5993537160710645 2 22 -0.2364042539058886 2 23 -1.0324511698005665 2 24 -0.8570989646373448 2 25 -0.20772921232200758 2 26 -0.5455111842249565 2 27 -0.1493225264641893 2 28 -0.6200451171072885 2 29 -0.7811212834957288 2 30 -1.1973212621404556 2 31 -0.896394350873642 2 32 -0.26590261814518357 2 33 -1.3554212550590101 2 34 -2.3458790594763492 2 35 -0.5951986294546714 2 36 -0.8038430189963037 2 37 -1.7316059213392556 2 38 -1.7028343996753499 2 39 -1.136718439772455 2 40 -1.9368693961220238 2 41 0.014188375389952768 2 42 0.16020167536625962 2 43 -0.24148893482414024 2 44 -0.2644155458302926 2 45 -1.144691469584533 2 46 -1.7544648602060597 2 47 -1.314499096526788 2 48 -1.1103563061841868 2 49 -1.434487576769623 2 50 -0.939254451288253 2 51 -0.8246044994773314 2 52 -0.5524384096726891 2 53 0.02321141038272856 2 54 -0.4257790881918666 2 55 0.7056151093225154 2 56 -1.4649645916245166 3 1 -0.3510709314989384 3 2 -0.018303717067041703 3 3 -0.2410779340213054 3 4 -0.13219813427149774 3 5 -0.5137690507740444 3 6 -0.6014461377254747 3 7 -0.3603072055536174 3 8 -0.29723153740342667 3 9 -0.41582404912227133 3 10 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0.7195690781235129 3 51 -0.5893103818302734 3 52 0.2946204138567232 3 53 0.29379964567684524 3 54 0.38598561769048345 3 55 0.0938504034401646 3 56 0.1468001142578359 4 1 -0.633423872675408 4 2 -1.230068422949394 4 3 -0.9351955810801229 4 4 -0.6851393107420805 4 5 -1.0667102272446343 4 6 -1.3308579024313545 4 7 -0.34854249967126094 4 8 -0.603113890344602 4 9 -0.5334711079458003 4 10 -1.2504418577166518 4 11 -1.134195078624856 4 12 -0.7479040200404725 4 13 -1.105612918493044 4 14 -0.7952009524694148 4 15 -0.6086394072910437 4 16 -0.8026904686208169 4 17 -0.870091807004961 4 18 -2.072425061162299 4 19 -1.0672881076531837 4 20 -2.393347064796167 4 21 -0.999353716071063 4 22 -2.636404253905887 4 23 -1.432451169800565 4 24 -1.8218048469902826 4 25 -1.3606703887925988 4 26 -1.4866876548131884 4 27 -0.8081460558759517 4 28 -1.3847509994602305 4 29 -1.7811212834957288 4 30 -0.5973212621404613 4 31 -1.6846296449912899 4 32 -0.9600202652040082 4 33 -0.5436565491766601 4 34 -0.28705553006458473 4 35 -1.2422574529840844 4 36 -1.2861959601727762 4 37 -1.378664744868665 4 38 -1.561657929087115 4 39 -1.2308360868312782 4 40 -1.442751749063202 4 41 -0.5269880951982842 4 42 -0.20450420698668026 4 43 -0.39443011129472794 4 44 -1.0761802517126462 4 45 -0.5093973519374764 4 46 -0.8132883896178242 4 47 -0.6909696847620808 4 48 -1.0397680708900694 4 49 -0.858016988534331 4 50 -0.7980779807000182 4 51 -0.23636920535968287 4 52 -0.9994972332020993 4 53 -0.7297297660878606 4 54 0.04480914710224937 4 55 -0.4355613612657194 4 56 0.6056236436696008 5 1 -0.2226167591323147 5 2 -0.06741452418058458 5 3 -0.36556493812526725 5 4 -0.10661673892266066 5 5 -0.21582103435817146 5 6 -0.4931014044833404 5 7 -0.3133851809298136 5 8 -0.1460687467057511 5 9 -0.3096680983698761 5 10 -0.06412174827752892 5 11 0.6571865903217926 5 12 -0.08046215957535452 5 13 0.402595015843481 5 14 0.4299700461626017 5 15 0.6827422616556049 5 16 0.4806200648949144 5 17 0.6385265240483946 5 18 0.15028355716875552 5 19 0.6328486912524269 5 20 0.022658407160051297 5 21 0.21733575041320563 5 22 0.03144800327605424 5 23 -0.7640517169961853 5 24 -0.2731044365935631 5 25 0.4962379559406429 5 26 0.40059004696519906 5 27 0.34164874576563875 5 28 0.39185638768067577 5 29 1.244596910758716 5 30 0.09323961337253905 5 31 2.065985950083949 5 32 0.5825241944403103 5 33 1.218039757252889 5 34 1.1966654001679728 5 35 1.46786566603096 5 36 0.8151720288833104 5 37 0.8563557749671773 5 38 1.6202846153725297 5 39 1.3011748570811719 5 40 1.2409691811693548 5 41 0.9912061592476817 5 42 0.3068501021788457 5 43 -0.8622823684766701 5 44 0.10699348289747945 5 45 -0.2788911959867235 5 46 1.2474503244724637 5 47 0.5426828460176729 5 48 1.3592743367706674 5 49 0.5088776763083516 5 50 0.5718262600660573 5 51 1.335723817896131 5 52 1.943047226442225 5 53 2.0511183871269125 5 54 1.4969295301391838 5 55 1.1439188028929692 5 56 1.4707399227393694 6 1 -0.4625486933353784 6 2 -0.7296380068805277 6 3 -0.2640334576941896 6 4 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0.19303434164823585 8 15 0.06194882800307511 8 16 0.09142717843801051 8 17 -0.16420945406378706 8 18 0.4687514094259413 8 19 -0.6202292841237735 8 20 -0.005111770678524863 8 21 0.6829992251054051 8 22 0.575360451976465 8 23 0.4381370654935566 8 24 0.7899598588920682 8 25 0.1452119641485794 8 26 0.3721358745985768 8 27 0.8153833558887555 8 28 0.27407252995153186 8 29 0.8659375400336842 8 30 0.7556199143301257 8 31 0.9153703550087116 8 32 0.134097381854815 8 33 0.4681081567056964 8 34 0.3600032934648283 8 35 1.298919017604149 8 36 1.0196863927683992 8 37 1.7625117257195697 8 38 1.0383420709128863 8 39 0.6397521484628399 8 40 0.6160717803485625 8 41 1.7906589636252441 8 42 1.3719663812486154 8 43 1.923216947528804 8 44 1.4767609247579436 8 45 1.572955589238994 8 46 2.3043586692057048 8 47 1.8972656093555642 8 48 2.5837613408746343 8 49 1.6243359526421415 8 50 2.060745548711747 8 51 2.116571971110904 8 52 2.0593262962096652 8 53 1.6467408221474322 8 54 2.315397382396366 8 55 1.8114974622636915 8 56 0.7115059966107768 9 1 0.09371516475774655 9 2 0.3377123257137029 9 3 0.21213062212842715 9 4 0.10189277481941161 9 5 0.4961239973542959 9 6 0.011655466552603855 9 7 0.3454414575479916 9 8 0.32776846259657333 9 9 0.22495135194724725 9 10 0.034451190411687094 9 11 0.5723557235141854 9 12 0.2884596163231592 9 13 -0.09291238373369026 9 14 -0.0668587064801045 9 15 0.01836594030254446 9 16 0.17565177736849336 9 17 -0.35618806368945144 9 18 9.706607628388042E-4 9 19 -0.7532506744981049 9 20 0.360396250711851 9 21 -0.06860505296945973 9 22 -1.246564681713373 9 23 -0.22282550135136603 9 24 -0.24720591650900303 9 25 -0.3483709235519541 9 26 0.10328560721889346 9 27 -0.4939749328813008 9 28 -0.5737884326153093 9 29 -0.22844748670428316 9 30 -0.4931768771137186 9 31 0.33422062238839345 9 32 0.7783487187532074 9 33 0.7122258037645182 9 34 0.3470353790263232 9 35 0.578330782310033 9 36 1.5109965531962075 9 37 1.321736324650054 9 38 0.9604008944422979 9 39 1.408468726002944 9 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28 -0.6087108272938053 120 29 -0.618137065418253 120 30 -0.24567133387647289 120 31 -0.0707128587646011 120 32 0.6134374105492171 120 33 0.6695428769352532 120 34 0.6056273967646817 120 35 0.828330782310033 120 36 0.614377927919044 120 37 0.7965145951600263 120 38 0.5488155285886371 120 39 0.5586904554929717 120 40 0.10115068996118737 120 41 -0.20618465186973012 120 42 0.2963566251510521 120 43 0.3702757710582141 120 44 0.2865170223189182 120 45 1.0824247427540605 120 46 0.8858507782444427 120 47 1.9641235720528414 120 48 0.4486106952505331 120 49 0.8656558952533331 120 50 0.46490623737745906 120 51 0.07338689220129169 120 52 0.4251799547462518 120 53 0.0329675079437024 120 54 0.13447916144945182 120 55 1.1558303173569513 120 56 0.6153797412305764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56.0 3 11.0 4 16.0 5 15.0 6 33.0 7 76.0 8 52.0 9 95.0 10 168.0 11 177.0 12 229.0 13 361.0 14 508.0 15 468.0 16 482.0 17 562.0 18 690.0 19 769.0 20 888.0 21 914.0 22 947.0 23 1177.0 24 1457.0 25 1697.0 26 1660.0 27 1761.0 28 1856.0 29 2258.0 30 2676.0 31 3049.0 32 3895.0 33 4890.0 34 6565.0 35 8815.0 36 11680.0 37 14407.0 38 13016.0 39 6400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 71.04963281843504 15.750822993162828 3.3943192369376214 9.805224951464506 11 36.34675445260404 17.889550097070988 12.00409386342534 33.759601586899635 12 41.0884612138094 9.026546805098338 31.64408711066093 18.240904870431333 13 23.34346247995273 12.147590107200134 33.48632565206381 31.02262176078332 14 18.10268422385414 16.40288680678653 46.366168650291215 19.12826031906812 15 22.48670549506204 14.051025576095213 45.13378914493121 18.32847978391154 16 26.212332236009118 13.064488900143495 33.103317295517854 27.619861568329533 17 30.16691989533215 14.469907993584874 19.856292732337298 35.50687937874567 18 31.354984384232292 15.60310627162995 27.051152190427956 25.990757153709797 19 36.69283362876678 19.27808727948004 24.0092428462902 20.019836245462987 20 28.991516839706254 21.95281505866464 29.57499788976112 19.480670211867984 21 34.26922427618806 19.37621338735545 26.142694353000763 20.21186798345573 22 33.246813539292646 19.484890689626067 26.44973410990124 20.818561661180045 23 41.561853712066856 16.873127083948848 23.53226691427848 18.03275228970582 24 35.99223432092513 21.1087195070482 24.322613319827806 18.576432852198867 25 31.437346956007765 23.792535675082327 18.80857890737144 25.961538461538463 26 28.55422959129792 15.285669025240258 25.621501742528253 30.538599640933572 27 20.71085291787695 18.837074201214683 25.274887773963563 35.17718510694481 28 27.59185311951998 25.963955969660475 21.78593310937863 24.658257801440918 29 32.40732913479014 18.395747918339744 20.965594488355382 28.23132845851473 30 27.32935676967659 18.227679421283078 27.79998730883939 26.642976500200945 31 36.37402278665806 15.937966126797068 26.8277459827143 20.86026510383057 32 42.277661397828986 11.547006919316956 25.617342728369195 20.55798895448486 33 43.96210437175823 12.229279136233725 26.27289086482481 17.535725627183233 34 31.15354489065775 14.414547917945677 38.42750386349684 16.004403327899738 35 22.9193802882528 27.097033813048682 37.632768900043416 12.350816998655102 36 30.740375751025205 29.67013171419186 25.743077852306325 13.846414682476608 37 30.328936914031463 26.56920387732401 26.30753747550188 16.794321733142645 38 31.475611436810247 23.720673826157938 25.530335958951305 19.273378778080506 39 30.76278645584789 24.124327935609074 28.336461680399584 16.77642392814346 40 33.641389115833924 18.458942990227804 24.743493161586045 23.156174732352223 41 30.72376101029396 18.577947575082245 29.13509498036719 21.563196434256607 42 22.131278066180453 23.28842744461076 30.890580379417603 23.689714109791183 43 29.277925828405195 14.184547761368341 26.291314087931454 30.24621232229501 44 24.451097804391217 11.86563044124517 29.79891281267253 33.88435894169108 45 29.61743526562865 18.3953947787667 26.66694989060475 25.320220064999894 46 25.541203713539122 19.69259204180918 30.306345733041574 24.459858511610122 47 25.768670754907486 17.585103782508423 31.676780988617402 24.969444473966693 48 28.12154461172068 15.004890703410734 30.884792038785402 25.988772646083184 49 26.251527549014398 14.759045746772223 32.120503692683705 26.86892301152967 50 21.012057929098525 14.831040129298428 32.912617229866235 31.244284711736807 51 18.57130710201305 15.873775056862872 39.09933465127649 26.455583189847587 52 19.111735052006892 16.13915300023398 40.268649097058265 24.48046285070087 53 15.979364994947614 17.411051427963624 40.34675317768441 26.26283039940435 54 19.31431367543188 17.23470342949536 39.25197855501659 24.199004340056167 55 17.55201958384333 16.117290192113245 34.565483476132194 31.765206747911236 56 16.206580963732335 20.782606844883365 26.80061297462966 36.21019921675464 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 4.0 11 8.0 12 39.5 13 75.0 14 79.0 15 97.5 16 116.0 17 321.0 18 526.0 19 633.0 20 740.0 21 769.0 22 833.0 23 868.0 24 1037.0 25 1206.0 26 1407.5 27 1609.0 28 1800.0 29 2055.5 30 2120.0 31 2103.0 32 2086.0 33 1650.0 34 1214.0 35 1445.0 36 1676.0 37 1152.0 38 703.5 39 779.0 40 717.5 41 656.0 42 868.5 43 1081.0 44 1040.5 45 1000.0 46 1296.0 47 1562.0 48 1532.0 49 1359.0 50 1186.0 51 1445.0 52 1704.0 53 2759.0 54 3944.0 55 4074.0 56 4456.0 57 4838.0 58 6493.5 59 8149.0 60 8312.0 61 8475.0 62 9554.0 63 11174.0 64 11715.0 65 9306.0 66 6897.0 67 5570.0 68 4243.0 69 3305.0 70 2367.0 71 1842.0 72 1450.0 73 1583.0 74 1120.5 75 658.0 76 492.5 77 327.0 78 253.5 79 138.5 80 97.0 81 85.5 82 74.0 83 53.0 84 32.0 85 26.0 86 20.0 87 16.5 88 7.5 89 2.0 90 2.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.004220477758082215 24 0.0 25 0.03376382206465772 26 0.09074027179876762 27 0.10656706339157591 28 0.12028361610534313 29 0.14771672153287752 30 0.23423651557356293 31 0.2606145015615768 32 0.2722208153963029 33 0.32286654849328944 34 0.31864607073520723 35 0.364016206634591 36 0.42732337300582424 37 0.40200050645733093 38 0.47374862834472864 39 0.5032919726513041 40 0.5581581835063729 41 0.5760952139782224 42 0.6109141554824007 43 0.6214653498776062 44 0.6204102304380856 45 0.6562842913817845 46 0.6689457246560311 47 0.7227568160715794 48 0.7596859964547986 49 0.7079851439182916 50 0.7702371908500042 51 0.728032413269182 52 0.7913395796404152 53 0.8029458934751413 54 0.8103317295517852 55 0.8662530598463746 56 0.8525365071326073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 94776.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.046931712669874 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98384957478349 19.198953321515997 2 10.880081142232973 5.885456233645649 3 5.00507138956074 4.061154722714611 4 2.980416634157759 3.224445007174812 5 1.9037216197238045 2.574491432430151 6 1.40828587032847 2.285388706001519 7 1.0337832566123117 1.957246560310627 8 0.8231255363969727 1.7810416139106948 9 0.5617539205742373 1.3674347936186377 >10 3.7021143793399394 19.318181818181817 >50 0.40181009596629474 8.102262176078332 >100 0.26527268471561205 14.554317548746518 >500 0.027307482250136534 5.238668017219549 >1k 0.02340641335725989 10.450958048451085 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 2646 2.791846036971385 No Hit CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 2105 2.221026420190766 No Hit CCTAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 1695 1.7884274499873385 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1210 1.27669452181987 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1132 1.1943952055372669 No Hit CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1117 1.1785684139444585 No Hit CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 944 0.9960327509074027 No Hit CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 932 0.9833713176331561 No Hit CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 683 0.7206465771925382 No Hit CCTAGAGGGGTGCGATGGCTTAGCAGGTCAAGGCACTCGCTACCAAGCTGTCGTGT 665 0.7016544272811682 No Hit CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 583 0.6151346332404828 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 581 0.6130243943614417 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCA 577 0.6088039166033595 No Hit CCTAGAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC 477 0.5032919726513041 No Hit CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTA 424 0.44737064235671475 No Hit CCTAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 418 0.44103992571959144 No Hit CCTAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 382 0.4030556258968515 No Hit CCTAGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 366 0.3861737148645227 No Hit CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 363 0.383008356545961 No Hit CCTAGAGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 359 0.3787878787878788 No Hit CCTAGAGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC 352 0.3714020427112349 No Hit CCTAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 322 0.3397484595256183 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 320 0.33763822064657717 No Hit CCTAGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGC 313 0.3302523845699333 No Hit CCTAGAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 305 0.32181142905376886 No Hit CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT 295 0.31126023465856334 No Hit CCTAGAGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC 285 0.3007090402633578 No Hit CCTAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 277 0.2922680847471934 No Hit CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 276 0.2912129653076728 No Hit CCTAGAGGGGAGCAATGCGCGTGGTACGCGGCAGTGACACCCGCCACAGCGCAGCT 275 0.29015784586815224 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 274 0.2891027264286317 No Hit CCTAGAGGGGAGCCAAGGCCAAGGAATTCACCAATGTTTATATCAAAAACTTTGGA 251 0.264834979319659 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 236 0.24900818772685068 No Hit CCTAGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 231 0.24373259052924792 No Hit CCTAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 213 0.22474044061787796 No Hit CCTAGAGGGGTTCAGATGTGACTGGAATCCCTTTCTGCTAATGTTCATGTGCCTGT 213 0.22474044061787796 No Hit CCTAGAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 209 0.22051996285979572 No Hit CCTAGAGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAA 208 0.2194648434202752 No Hit CCTAGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 208 0.2194648434202752 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCA 195 0.205748290706508 No Hit CCTAGAGGGACAGTAATAAAATGCATGTGTGACGTTAAGACTAGTGTCTGTCATAT 191 0.20152781294842576 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCC 190 0.20047269350890523 No Hit CCTAGAGGGGGTACTTGAGAGATGGCTCAGAGGTTAAGACCACTGACTGCTCTTCC 184 0.1941419768717819 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA 179 0.1888663796741791 No Hit CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.18675614079513803 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 177 0.18675614079513803 No Hit CCTAGAGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCACTGCTCTTCCG 176 0.18570102135561745 No Hit CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 173 0.18253566303705582 No Hit CCTAGAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA 173 0.18253566303705582 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCACTTCCG 170 0.17937030471849413 No Hit CCTAGAGGGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGAC 165 0.17409470752089137 No Hit CCTAGAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC 163 0.17198446864185027 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCAA 159 0.16776399088376803 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC 156 0.16459863256520638 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC 154 0.16248839368616527 No Hit CCTAGAGGGGGTGCAGCGGGAGGTTTTTAGGAGGGCTGTTTTGCCAAGGGTCAATC 154 0.16248839368616527 No Hit CCTAGAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 151 0.15932303536760362 No Hit CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 150 0.15826791592808306 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 149 0.1572127964885625 No Hit CCTAGAGGGGAATTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAA 139 0.146661602093357 No Hit CCTAGAGGGGGGACTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC 139 0.146661602093357 No Hit CCTAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 126 0.13294504937958976 No Hit CCTAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 126 0.13294504937958976 No Hit CCTAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA 125 0.1318899299400692 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCC 123 0.1297796910610281 No Hit CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA 121 0.127669452181987 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC 120 0.12661433274246645 No Hit CCTAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 0.1255592133029459 No Hit CCTAGAGGGGGGAATTCCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 118 0.12450409386342534 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTC 117 0.12344897442390479 No Hit CCTAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA 115 0.12133873554486367 No Hit CCTAGAGGGCAGCACCACATGCTCCATGTCAAGTGTGTACGTGGAGACCACTGGCT 115 0.12133873554486367 No Hit CCTAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC 113 0.11922849666582258 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC 110 0.1160631383472609 No Hit CCTAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 0.11500801890774036 No Hit CCTAGAGGGGATTTATTTTTTCTGGAGTGACAAAAGATTTAGGAAGGCTCCGCATG 106 0.11184266058917869 No Hit CCTAGAGGGGGGATGCAGGCATGCTTCCCACTGTTCAGAGCTCTGGCTTCCATCTG 105 0.11078754114965815 No Hit CCTAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAA 104 0.1097324217101376 No Hit CCTAGAGGGAGGCTATTTGAATTCATTTAAATGAAATACTGAAGAAATTTGTTCCT 103 0.10867730227061703 No Hit CCTAGAGGGCAAATCTGGAACTGTCTTTCTCCCCAGCTCTGCCCCCTTCATTCCTA 102 0.10762218283109648 No Hit CCTAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA 101 0.10656706339157591 No Hit CCTAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 100 0.10551194395205538 No Hit CCTAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC 100 0.10551194395205538 No Hit CCTAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA 100 0.10551194395205538 No Hit CCTAGAGGGGCCTTTTCCTCTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 100 0.10551194395205538 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC 99 0.10445682451253482 No Hit CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTG 99 0.10445682451253482 No Hit CCTAGAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 99 0.10445682451253482 No Hit CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 97 0.1023465856334937 No Hit CCTAGAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT 96 0.10129146619397315 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTC 96 0.10129146619397315 No Hit CCTAGAGGGAGCCATTCCTAGAATTCCACTGTCACCCATTCGAAGAACTGGTCCAG 96 0.10129146619397315 No Hit CCTAGAGGGGAAGTAGAAACAGAGAAGGAAAGAATTTTGTGGTTGCTTGCTGAATT 96 0.10129146619397315 No Hit CCTAGAGGGGGGAATTGGCCTGGAATTACATCTGGAGTGGGAAAGGAGTTGTAGGG 96 0.10129146619397315 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTT 96 0.10129146619397315 No Hit CCTAGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA 95 0.10023634675445262 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.004220477758082215 0.0 0.0 0.0 0.0 11 0.00844095551616443 0.0 0.0 0.0 0.0 12 0.00844095551616443 0.0 0.0 0.0 0.0 13 0.009496074955684983 0.0 0.0 0.0 0.0 14 0.014771672153287752 0.0 0.0 0.0 0.0 15 0.014771672153287752 0.0 0.0 0.0 0.0 16 0.014771672153287752 0.0 0.0 0.0 0.0 17 0.014771672153287752 0.0 0.0 0.0 0.0 18 0.014771672153287752 0.0 0.0 0.0 0.0 19 0.015826791592808306 0.0 0.0 0.0 0.0 20 0.01688191103232886 0.0 0.0 0.0 0.0 21 0.017937030471849413 0.0 0.0 0.0 0.0 22 0.017937030471849413 0.0 0.0 0.0 0.0 23 0.018992149911369965 0.0 0.0 0.0 0.0 24 0.024267747108972736 0.0 0.0 0.0 0.0 25 0.024267747108972736 0.0 0.0 0.0 0.0 26 0.024267747108972736 0.0 0.0 0.0 0.0 27 0.02848822486705495 0.0 0.0 0.0 0.0 28 0.03376382206465772 0.0 0.0 0.0 0.0 29 0.034818941504178275 0.0 0.0 0.0 0.0 30 0.03692918038321938 0.0 0.0 0.0 0.0 31 0.03692918038321938 0.0 0.0 0.0 0.0 32 0.04009453870178104 0.0 0.0 0.0 0.0 33 0.04009453870178104 0.0 0.0 0.0 0.0 34 0.041149658141301594 0.0 0.0 0.0 0.0 35 0.04853549421794547 0.0 0.0 0.0 0.0 36 0.04853549421794547 0.0 0.0 0.0 0.0 37 0.04853549421794547 0.0 0.0 0.0 0.0 38 0.049590613657466025 0.0 0.0 0.0 0.0 39 0.051700852536507136 0.0 0.0 0.0 0.0 40 0.05275597197602769 0.0 0.0 0.0 0.0 41 0.0569764497341099 0.0 0.0 0.0 0.0 42 0.0569764497341099 0.0 0.0 0.0 0.0 43 0.05908668861315101 0.0 0.0 0.0 0.0 44 0.06014180805267156 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCAATG 20 4.048118E-4 50.287163 50 GAACAAG 20 4.048118E-4 50.287163 49 TGGTCTG 25 2.0036801E-5 50.28716 50 TTGCAAT 15 0.0082188975 50.28716 49 AGCTTGT 15 0.0082188975 50.28716 49 GTCATAT 25 2.0036801E-5 50.28716 50 AATTCTC 15 0.0082188975 50.28716 50 TTGTAGG 15 0.0082188975 50.28716 49 CCCGGTC 15 0.0082188975 50.28716 49 TGTCATA 25 2.0036801E-5 50.28716 49 GGATTAA 25 2.0036801E-5 50.28716 49 TTATATG 25 2.0036801E-5 50.28716 50 CTGAATT 15 0.0082188975 50.28716 50 TGTAGGG 15 0.0082188975 50.28716 50 TTGATCC 15 0.0082188975 50.28716 50 AGATGAA 15 0.0082188975 50.28716 50 GTAGGCT 15 0.0082188975 50.28716 49 GATTAAA 25 2.0036801E-5 50.28716 50 ACAACTG 15 0.0082188975 50.28716 50 TGTCGTG 85 0.0 50.28716 49 >>END_MODULE