FastQCFastQC Report
Tue 31 May 2016
SRR212711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321437
Sequences flagged as poor quality0
Sequence length58
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT51491.6018691065434285No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC23570.7332696609288912No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA23190.7214477487034785No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT16940.5270084028907687No Hit
GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC15350.4775430333160153No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG14140.4398995759666746No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13450.4184334721889515No Hit
GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT10540.3279025127785538No Hit
GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC9240.28745912884951014No Hit
GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCCCA9100.28310368750330545No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT8040.25012677445346987No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC7700.2395492740412585No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7190.22368302342294136No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT7110.22119419979653868No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA6730.20937228757112591No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.20781677280462424No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC6170.1919505221863071No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5590.17390655089488766No Hit
GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5150.16021802094967286No Hit
GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCACAC5100.1586625061831712No Hit
GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4720.14684059395775845No Hit
GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4600.14310735851815443No Hit
GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA4460.1387519171719497No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG4460.1387519171719497No Hit
GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4370.1359519905922467No Hit
GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG4270.13284096105924334No Hit
GTGGTAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT4180.1300410344795403No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA4020.12506338722673493No Hit
GTGGTAGGGGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATAT3990.12413007836683393No Hit
GTGGTAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3930.12226346064703193No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC3900.12133015178713091No Hit
GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACGCA3890.12101904883383059No Hit
GTGGTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3850.11977463702062925No Hit
GTGGTAGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAGA3780.11759691634752689No Hit
GTGGTAGGGCCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3680.11448588681452353No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3680.11448588681452353No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3630.11293037204802185No Hit
GTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTCCG3620.1126192690947215No Hit
GTGGTAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3600.11199706318812085No Hit
GTGGTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3510.10919713660841783No Hit
GTGGTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3490.10857493070181715No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3390.1054639011688138No Hit
GTGGTAGGGGAAGTGTTCCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTT3370.10484169526221312No Hit
GTGGTAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG3360.1045305923089128No Hit
GTGGTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT3340.10390838640231212No Hit
GTGGTAGGGGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCGACTCAGACGCC3310.1029750775424111No Hit
GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC3300.10266397458911078No Hit
GTGGTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT3290.10235287163581044No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT3280.1020417686825101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT150.00719367552.03971552
CCACGTA150.00719367552.03971551
TCAGTCT251.6450575E-552.03971551
CGTTCGA203.430967E-452.0397152
TCGATAC203.430967E-452.0397152
ATCGATA203.430967E-452.0397151
AACCTAC353.8531653E-852.03160549
GTCAAAC150.007202540552.0235148
CGAATCT307.952167E-752.0235146
TCCCGAG150.007206976452.0154145
CTACAAT203.438889E-452.0154145
TACTAAG150.007206976452.0154145
ACGTATA203.438889E-452.0154140
CCAAATA150.007206976452.0154143
GTAACAC203.438889E-452.0154137
TCTATAC251.6496128E-552.0154139
TATCCGG203.438889E-452.0154144
CGTATAG203.4415332E-452.00731741
AGATAGA150.007211414652.00731742
GCACGAC203.4415332E-452.00731742
Produced by FastQC (version 0.11.5)